Characterization of Mongrel Dog Semen of Mizoram

Zorinkimi, Amy; Ahmed, Feroz; Lalrintluanga, K.; Kuotsu, Neithono

doi:10.20546/ijcmas.2017.612.128

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…The average concentration observed in the present study is 280 million per mL of semen and is in congruent with previous studies (Zorinkimi et al, 2017). Sabarinathan, (2015) observed mean concentration of 332 million per mL of semen.…”

Section: Resultssupporting

confidence: 93%

Evaluation of Metformin Supplemented Diluent on Cryopreservation of Canine Semen

Joshy,

Rangasamy,

Sathiamoorthy

et al. 2024

IJAR

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Background: Cryopreservation of semen in canines is getting more advanced lately, however the fertility following the insemination of the frozen thawed semen is lower than other species. The higher content of PUFA and lipid peroxidation leading to production of reactive oxygen species is one of the reasons for the same. Antioxidants play a role in improving the post thaw semen quality in canines. Methods: Semen collected from 10 male dogs were further divided into two aliquots. The first aliquot was extended with TRIS-fructose egg yolk extender and the second one was extended with TRIS-fructose egg yolk extender supplemented with 50 µM of metformin. The extended semen was cryopreserved using conventional methods. Subsequently, both routine semen parameters and advanced semen parameters including viability, acrosomal integrity, DNA integrity and mitochondrial reactive oxygen species (ROS) were measured in the spermatozoa through the utilization of flow cytometry. Result: Present study indicated that, post thaw motility was significantly higher in the metformin supplemented group in comparison with control while, the mitochondrial ROS was significantly lower in metformin supplemented group. Our study concludes metformin could be effectively used to reduce the ROS produced during the process of cryopreservation of the canine semen.

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Section: Resultssupporting

confidence: 93%

Evaluation of Metformin Supplemented Diluent on Cryopreservation of Canine Semen

Joshy,

Rangasamy,

Sathiamoorthy

et al. 2024

IJAR

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Criopreservación de semen canino (Canis familiaris) en pajilla francesa en el municipio de Medellín, Antioquia

Arango-Múnera

Castrillón-Zuluaga

Correa-Rendón

et al. 2020

Rev Colombiana Cienc Anim. RECIA

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El objetivo de este trabajo fue aplicar la metodología de criopreservación de semen canino en pajilla francesa en el municipio de Medellín- Antioquia. Se realizó una colecta seminal a ocho caninos adultos, por método de mano enguantada, cada eyaculado fue evaluado macroscópica y microscópicamente y posteriormente diluido con Triladyl® a una concentración final de 80 x 106 espermatozoides/ml. Se empacaron en pajillas de 0,5 ml y fueron sometidas a criopreservación con un descenso de temperatura rápido, sometiendo las pajillas a vapores de nitrógeno durante 25 minutos y luego transferidas directamente a nitrógeno líquido (método de pajilla francesa). En el semen fresco, se observó una apariencia blanquecina lechosa, un volumen de 2.27 ml (±1.40) una concentración de 388.5 x106 espermatozoides/ mL (±228.069), una motilidad individual de 79% (± 4%) y un vigor de 3.96 (±0.327). Se obtuvieron 123 pajillas, de las cuales se tomaron 27 aleatoriamente y se les evaluó la motilidad individual (51 ± 19%) y vigor (2.89 ± 1.02). En conclusión, la aplicación de la metodología de criopreservación rápida por pajilla francesa, usando Triladyl® como crioprotector, se sugiere como protocolo eficiente para la criopreservación de semen canino en el municipio de Medellín.

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Characterization of Mongrel Dog Semen of Mizoram

Cited by 2 publications

References 15 publications

Evaluation of Metformin Supplemented Diluent on Cryopreservation of Canine Semen

Evaluation of Metformin Supplemented Diluent on Cryopreservation of Canine Semen

Criopreservación de semen canino (Canis familiaris) en pajilla francesa en el municipio de Medellín, Antioquia

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