Characterization of Missense Mutations in the SUP45 Gene of Saccharomyces cerevisiae Encoding Translation Termination Factor eRF1*

Moskalenko, Svetlana E.; Zhouravleva, Galina A.; Soom, M. Y.; Chabelskaya, Svetlana; Volkov, Kirill V.; Zemlyanko, O. M.; Mironova, L. N.; Инге-Вечтомов, С. Г.

doi:10.1023/b:ruge.0000029148.58151.91

Cited by 13 publications

(20 citation statements)

References 25 publications

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“…Escherichia coli strain used was XL1-Blue (recA1, endA1, gyrA96, thi-1, hsdR17, supE44, relA1 [F', proAB, lacIq, D(lacZ)M15, Tn10(tet)]). 25 The Saccharomyces cerevisiae strains used in this study (Table 1) 23,24 used in this study included nonsense alleles (sup45-n), such as sup45-101 (266EgTAA), sup45-102 (53YgTAA), sup45-104 (283LgTAA), sup45-105 (385EgTAA) and sup45-107 (317LgTGA), as well as missense alleles (sup45-m), such as sup45-103 (L21S), sup45-111 (R65C), sup45-113 (M48I), sup45-115 (S70F) and sup45-116 (R62T).…”

Section: Methodsmentioning

confidence: 99%

“…22 Another variant of [PSI + ] used in the same study did not show synthetic lethality, suggesting that such an effect could be variant-specific. 21 In the present study, we demonstrate synthetic lethality of well-characterized missense and nonsense-alleles of SUP45 23,24 with different variants of [PSI + ]. Our data show that synthetic lethality is a common trend observed for various mutant/prion combinations.…”

Section: Introductionmentioning

confidence: 94%

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Prion-Dependent Lethality ofsup45Mutants inSaccharomyces cerevisiae

Kiktev

Vechtomov

Zhouravleva

2007

Prion

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In yeast Saccharomyces cerevisiae translation termination factors eRF1 (Sup45) and eRF3 (Sup35) are encoded by the essential genes SUP45 and SUP35 respectively. Heritable aggregation of Sup35 results in formation of the yeast prion [PSI(+)]. It is known that combination of [PSI(+)] with some mutant alleles of the SUP35 or SUP45 genes in one and the same haploid yeast cell causes synthetic lethality. In this study, we perform detailed analysis of synthetic lethality between various sup45 nonsense and missense mutations on one hand, and different variants of [PSI(+)] on the other hand. Synthetic lethality with sup45 mutations was detected for [PSI(+)] variants of different stringencies. Moreover, we demonstrate for the first time that in some combinations, synthetic lethality is dominant and occurs at the postzygotic stage after only a few cell divisions. The tRNA suppressor SUQ5 counteracts the prion-dependent lethality of the nonsense alleles but not of the missense alleles of SUP45, indicating that the lethal effect is due to the depletion of Sup45. Synthetic lethality is also suppressed in the presence of the C-proximal fragment of Sup35 (Sup35C) that lacks the prion domain and cannot be included into the prion aggregates. Remarkably, the production of Sup35C in a sup45 mutant strain is also accompanied by an increase in the Sup45 levels, suggesting that translationally active Sup35 up-regulates Sup45 or protects it from degradation.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

Prion-Dependent Lethality ofsup45Mutants inSaccharomyces cerevisiae

Kiktev

Vechtomov

Zhouravleva

2007

Prion

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“…1c), however, it caused the strengthening of sup pression in all mutants studied. This effect is the least prominent in sup45 115, in which the ade1 14 muta tion suppression absence is usual [24]. The absence of correlation between the revealed effect and the change of the content of eRF1 and/or eRF3 proteins was detected by estimating the content of these proteins in yeast cells with mutations sup45 103 and sup45 113 and comparing with wild type UPF1 gene and its dele tion.…”

Section: Upf1 Gene Deletion Leads To Increasementioning

confidence: 97%

“…cerevisiae yeast strains used in this work are spec ified in Table 1. Strain 1A D1628 contains a deletion in SUP45 gene recovered with SUP45 wild type plas mid or with mutant sup45 gene alleles as described ear lier [3,24]. OT56 and OT55 strains are isogene deriv atives of the 74 D694 strain [25] [26].…”

Section: S Cerevisiae Strainsmentioning

confidence: 99%

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Influence of UPF genes on severity of SUP45 mutations

Zhouravleva

Gryzina

2012

Mol Biol

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Eukaryotic cells possess a special mechanism for the degradation of mRNAs containing prema ture termination codons (PTCs), referred to as NMD (nonsense mediated mRNA decay). The strength of this pathway depends on the recognition of the PTCs by translational machinery and the interaction of trans lation termination factors eRF1 and eRF3 with Upf1, Upf2 and Upf3 proteins in Sachromyces cerevisiae yeast. Previously, we have shown that the decrease of eRF1 protein amounts in sup45 nonsense mutants leads to the impairment of NMD. Here we show that the deletion of UPF1 or UPF2 genes leads to an increase in the viability of sup45 mutants, while the effect of UPF3 gene deletion is allele specific. Two hybrid data have shown that amino acid residues 1 555 of Upf1 protein interact with eRF1. Any UPF gene deletion leads to allosupression of the adel1 14 mutation without a change in eRF1 content. The Upf1 depletion does not influence the synthetic lethality of sup45 mutations and the [PSI + ] prion. It is possible that the absence of Upf1 (or its activator Upf2) leads to a more effective formation of the translation termination complex and consequently to the increased viability of the cells containing mutant termination factors.

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Current awareness on yeast

2005

Yeast

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In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Books, Reviews & Symposia; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (4 weeks journals ‐ search completed 13th. Oct. 2004)

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Characterization of Missense Mutations in the SUP45 Gene of Saccharomyces cerevisiae Encoding Translation Termination Factor eRF1*

Cited by 13 publications

References 25 publications

Prion-Dependent Lethality ofsup45Mutants inSaccharomyces cerevisiae

Prion-Dependent Lethality ofsup45Mutants inSaccharomyces cerevisiae

Influence of UPF genes on severity of SUP45 mutations

Current awareness on yeast

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