Characterization of milk protein concentrate solubility using focused beam reflectance measurement

Abstract

-Milk protein concentrate (MPC) is a newly developed dairy powder with high protein content (40-90 wt%). MPC is known to have relatively poor dissolution properties, rendering traditional testing methods ineffective for characterizing their functionality. To date, various techniques have been used to quantitatively measure the properties of MPC. Mostly, they are conducted offline with considerable sample preparation that influences the reproducibility of the measurement. Here, a methodology to characterize the solubility of MPC using Focused Beam Reflectance Measurement (FBRM) is presented. FBRM provides the ability to monitor in situ the changes in chord length with time over a wide range of suspension concentrations, which directly reflects the solubility of MPC in this case. Faster rate of the chord length reduction implies a better solubility, as more particles break down and dissolve in solution. In this study, the effect of water temperature was investigated for MPC powders. A characteristic dissolution profile for different MPC powders was subsequently established. milk protein concentrate / dissolution / solubility / particle size / FBRMRésumé -Caractérisation de la solubilité de concentrés de protéines laitières par la technique FBRM. Le concentré de protéines totales de lait (CPT) est une poudre laitière nouvellement développée ayant une teneur élevée en protéine (40-90 %p). Ce produit est connu pour avoir des propriétés de dissolution relativement faibles, rendant les méthodes de mesure traditionnelles inefficaces pour caractériser ses fonctionnalités. À ce jour, différentes techniques ont été utilisées pour mesurer quantitativement les propriétés des CPT. Elles sont majoritairement effectuées hors ligne avec une longue préparation des échantillons qui influence la reproductibilité de la mesure. Article published by EDP Sciences Une méthode utilisant la technique FBRM pour caractériser la solubilité des CPT est présentée. Cette méthode de mesure de la réflectance d'un faisceau focalisé de lumière permet de suivre in situ les changements de longueur de corde au cours du temps sur une large gamme de concentrations de suspensions qui renvoient directement à la solubilité du CPT dans ce cas. Une vitesse plus rapide de réduction de la longueur de corde signifie une meilleure solubilité puisque plus de particules se cassent et se dissolvent en solution. Dans cette étude, l'effet de la température de l'eau a été étudié sur les poudres de CPT. Un profil de dissolution caractéristique de différentes poudres de CPT a été établi par la suite.concentré de protéines totales de lait / dissolution / solubilité / taille de particules / FBRM

Rehydration characteristics of milk protein concentrate powders

“…Analysis of PSD, after 90 min and 24 h rehydration, confirmed the results of numerous previous studies which reported the presence of large poorly-dispersible particles in high-protein MPCs after reasonable periods of rehydration (Fang et al, 2010(Fang et al, , 2011Mimouni et al, 2009Mimouni et al, , 2010b. In addition, it is clear that this phenomenon is not restricted to powders with >80% protein, with MPC60 and MPC70 also displaying similar behaviour.…”

“…8. Increasing the temperature of reconstitution to $50°C is common industrial practice when a dairy powder is difficult to reconstitute; however, the correlation between increased temperature and improved rehydration is often weak (Fang et al, 2010;Richard et al, 2013), as it depends on the composition, processand storage-history of the powder. A higher temperature of reconstitution resulted in a strong decrease in sediment height for MPC90 rehydrated in water (Fig.…”

Functional Characteristics of Milk Protein Concentrates and Their Modification

“…Another approach developed by Fang et al (2010Fang et al ( , 2011 used focused beam reflectance measurements. All these methods have managed to give insights into the process of solubility, factors and interactions involved in rehydration process and subsequently draw correlations with loss of solubility in MPC as also done by Mata et al (2011) using small angle X-ray scattering method.…”

Effect of sodium chloride addition during diafiltration on the solubility of milk protein concentrate

“…The second method attempted to characterize the solubility of MPC using focused beam reflectance measurements (FBRM), which provides the ability to monitor changes in chord length over time under a variety of suspension concentrations (Fang et al, 2010). A characteristic dissolution profile for different MPC powders was able to be established, but it was recognized that further investigation must be conducted to confirm FBRM as a method suitable for MPC solubility analysis, especially given that MPCs vary widely in composition and dissolution characteristics.…”