Characterization of microtiterplates with integrated optical sensors for oxygen and pH, and their applications to enzyme activity screening, respirometry, and toxicological assays

Arain, Sarina; John, Gernot T.; Krause, Christian; Gerlach, Jochen; Wolfbeis, Otto S.; Klimant, Ingo

doi:10.1016/j.snb.2005.07.056

Cited by 75 publications

(35 citation statements)

References 14 publications

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“…The detection system was further developed to allow for the ability to monitor 12 mini stirred-tank bioreactors (Ge et al, 2006) and both 24-well microplates and a discrete 24-unit microbioreactor system (Harms et al, 2006). Microplates with integrated sensors were investigated in detail with respect to their properties in cellular cultivation (Arain et al, 2006;John et al, 2003a,b). Several other designs for microbioreactors with integrated optical sensors for oxygen and pH have been described, like one fabricated from poly(dimethylsiloxane) (PDMS) and glass (Zanzotto et al, 2004) or from PDMS and poly(methyl methacrylate) (PMMA; Zhang et al, 2006) and also a microfluidic PDMS bioreactor (Mehta et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Multiplex bacterial growth monitoring in 24‐well microplates using a dual optical sensor for dissolved oxygen and pH

Kocincová

Nagl

Arain

et al. 2008

Biotech & Bioengineering

Self Cite

104

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Non-invasive, simultaneous optical monitoring of oxygen and pH during bacterial cultivation in 24-well microplates is presented using an integrated dual sensor for dissolved oxygen and pH values. The dual sensor is based on oxygen-sensitive organosilica microparticles and pH-sensitive microbeads from a polymethacrylate derivative embedded into a polyurethane hydrogel. The readout is based on a phase-domain fluorescence lifetime-based method referred to as modified frequency domain dual lifetime referencing using a commercially available detector system for 24-well microplates. The sensor was used for monitoring the growth of Pseudomonas putida bacterial cultures. The method is suitable for parallelized, miniaturized bioprocessing, and cell-based high-throughput screening applications.

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Section: Introductionmentioning

confidence: 99%

Multiplex bacterial growth monitoring in 24‐well microplates using a dual optical sensor for dissolved oxygen and pH

Kocincová

Nagl

Arain

et al. 2008

Biotech & Bioengineering

Self Cite

104

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“…One setup uses an electro-optical measurement system (Precision Sensing GmbH, Regensburg, Germany) to read out the optical pH-and pO 2 -sensors [1]. A second setup uses an inverted light microscope (imaging MICroscope or iMIC, Till Photonics, Gräfelfing, Germany) to make the wide variety of optical analysis methods accessible.…”

Section: Methodsmentioning

confidence: 99%

Automated live cell screening system based on a 24-well-microplate with integrated micro fluidics

et al. 2007

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In research, pharmacologic drug-screening and medical diagnostics, the trend towards the utilization of functional assays using living cells is persisting. Research groups working with living cells are confronted with the problem, that common endpoint measurement methods are not able to map dynamic changes. With consideration of time as a further dimension, the dynamic and networked molecular processes of cells in culture can be monitored. These processes can be investigated by measuring several extracellular parameters. This paper describes a high-content system that provides real-time monitoring data of cell parameters (metabolic and morphological alterations), e.g., upon treatment with drug compounds. Accessible are acidification rates, the oxygen consumption and changes in adhesion forces within 24 cell cultures in parallel. Addressing the rising interest in biomedical and pharmacological high-content screening assays, a concept has been developed, which integrates multi-parametric sensor readout, automated imaging and probe handling into a single embedded platform. A life-maintenance system keeps important environmental parameters (gas, humidity, sterility, temperature) constant.

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“…The determination of oxygen partial pressure (pO 2 ) is a major task in chemical and biotechnological process control, pharmaceutical screening, medical chemistry and diagnosis (e.g., blood gas analysis), and environmental analysis (e.g., cellular respirometry) [78,79]. Typical luminescent oxygen-sensitive probes applied in sensors consist of transition metal-ligand complexes, particularly of Ru 2+ , Os 2+ , and Ir 3+ , or platinum and palladium porphyrins [80,81].…”

Section: Oxygen Sensorsmentioning

confidence: 99%

“…These can be immobilized on the tip of a waveguide by sol-gel techniques or by embedding in polymeric hydrogels [89]. Disposable microplates (e.g., a 96-well format) with integrated fluorescent pH sensors can be used for screening of enzyme reactions or monitoring the proliferation of cells [79].…”

Section: Ph Sensorsmentioning

confidence: 99%

Luminescent Chemical and Physical Sensors Based on Lanthanide Complexes

Spangler

Schäferling

2010

Lanthanide Luminescence

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Luminescent lanthanide complexes (LLCs) are versatile probes for the determination of a variety of analytes such as metal ions, anions, hydrogen peroxide, ATP, pH, or oxygen. Antenna chromophores have been introduced as ligands for sensitizing lanthanide emission. The ligand system or the lanthanide ion can act as receptors for reversible binding of analytes. Thus, the response of luminescence emission can occur via ligand or metal-centered processes, which will both be discussed. LLCs, particularly Eu 3+ and Tb 3+ complexes, have been incorporated in solid state matrices, e.g., polymer hydrogels or silica to obtain optical sensor layers. A selection of such sensitive materials will be outlined, including sensors for the analytically relevant parameters oxygen, pH, hydrogen peroxide, humidity, and temperature.

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Characterization of microtiterplates with integrated optical sensors for oxygen and pH, and their applications to enzyme activity screening, respirometry, and toxicological assays

Cited by 75 publications

References 14 publications

Multiplex bacterial growth monitoring in 24‐well microplates using a dual optical sensor for dissolved oxygen and pH

Multiplex bacterial growth monitoring in 24‐well microplates using a dual optical sensor for dissolved oxygen and pH

Automated live cell screening system based on a 24-well-microplate with integrated micro fluidics

Luminescent Chemical and Physical Sensors Based on Lanthanide Complexes

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