Characterization of microsatellite loci in caribou <i>Rangifer tarandus</i>, and their use in other artiodactyls

Wilson, Gregory; Strobeck, Curtis; Wu, Lei; Coffin, J.

doi:10.1046/j.1365-294x.1997.00237.x

Cited by 213 publications

(134 citation statements)

References 7 publications

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“…All individuals were genotyped using microsatellite primers designed for white-tailed deer. This panel included markers Eth152 (Steffen et al 1993); N and Q (Jones et al 2000); Srcrsp10 (Bhebhe et al 1994); IGF-1 (Kirkpatrick 1992); OCAM ); RT7, RT9, RT27, and RT30 (Wilson et al 1997); and BM1225, BM4107, and CSN3 (Bishop et al 1994). Null alleles were previously found when using CSN3 with Illinois white-tailed deer, and as a result, we selected a redesigned reverse primer for the locus (Kelly et al 2011).…”

Section: Methodsmentioning

confidence: 99%

Female Philopatry and Extreme Spatial Genetic Heterogeneity in White-Tailed Deer

Purdue¹,

Smith²,

Patton³

2000

Journal of Mammalogy

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Section: Methodsmentioning

confidence: 99%

Female Philopatry and Extreme Spatial Genetic Heterogeneity in White-Tailed Deer

Purdue¹,

Smith²,

Patton³

2000

Journal of Mammalogy

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“…DNA was amplified using 9 polymorphic, microsatellite markers (Rt6, Rt7, Rt24, Rt30 (Wilson et al, 1997); Map2C, BM848 (Moore et al, 1992); BM888, RT5 (McLoughlin et al, 2004); BMS1788 (Cronin et al, 2005). Each reaction was composed of a 10-μl volume containing: 1x PCR buffer, 2.0 μM MgCl 2 , 0.…”

Section: Geneticsmentioning

confidence: 99%

Comparative woodland caribou population surveys in Slate Islands Provincial Park, Ontario

Carr

Rodgers²,

Kingston³

et al. 2012

Ran

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Abstract:We evaluated three methods of estimating population size of woodland caribou (boreal ecotype) on the Slate Islands in northern Ontario. Located on the north shore of Lake Superior, the Slate Islands provide a protected and closed population with very limited predator influence that is ideal for a comparison of survey methods. Our objective was to determine the costs and benefits of three population estimation techniques: (1) forward looking infrared (FLIR) technology to count the number of caribou on regular-spaced transects flown by fixed-wing aircraft; (2) observers to count the number of caribou seen or heard while walking random transects in the spring; and, (3) mark-recapture sampling of caribou pellets using DNA analysis. FLIR and the genetics 3-window approach gave much tighter confidence intervals but similar population estimates were found from all three techniques based on their overlapping confidence intervals. There are various costs and benefits to each technique that are discussed further. Understanding the costs and benefits of different population estimation techniques is necessary to develop cost-effective programs for inventorying and monitoring this threatened species not only on the Slate Islands but for other populations as well.

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“…We genotyped individuals using 13 microsatellite primers that were optimized for use in whitetailed deer (Anderson et al, 2002), but were originally developed for cattle (Bos taurus), sheep (Ovis aries), goats (Capra hircus), and caribou (Rangifer tarandus). This panel included markers BM848, BM1225, BM4107, BM6506, CSN3 (Bishop et al, 1994), BM4208 (Talbot et al, 1996), IGF-1 (Kirkpatrick, 1992), OBCAM (Fries et al, 1993), OarFcb304 (Buchanan et al, 1993), RT20, RT23, RT27 (Wilson et al, 1997), and Srcrsp-10 (Bhebhe et al, 1994). We labeled forward primers with fluorescent dyes (NED, HEX, FAM) and separated microsatellite fragments on an ABI 3730XL capillary sequencer (Applied Biosystems, Foster City, CA, USA).…”

Section: Laboratory Proceduresmentioning

confidence: 99%

Microsatellites behaving badly: empirical evaluation of genotyping errors and subsequent impacts on population studies

et al. 2011

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ABSTRACT. Microsatellites are useful tools for ecological studies because they can be used to discern population structure, dispersal patterns and genetic relationships among individuals. However, they can also yield inaccurate genotypes that, in turn, bias results, promote biological misinterpretations, and create repercussions for population management and conservation programs. We used empirical data from a large-scale microsatellite DNA study of white-tailed deer (Odocoileus virginianus) to identify sources of genotyping error, evaluate corrective measures, and provide recommendations to prevent bias in population studies. We detected unreported mutations that led to erroneous genotypes in five of 13 previously evaluated microsatellites. Of the five problematic markers, two contained mutations that resulted in null alleles, and three contained mutations that resulted in imperfect repeats. These five microsatellites had error rates that were four times greater on average than those observed in the remaining eight. 2535©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 10 (4): 2534-2553 (2011) Genotyping errors in population studiesMethodological corrections, such as primer redesign, reduced errors up to 5-fold in two problematic loci, although analytical corrections (computational adjustment for errors) were unable to fully prevent bias and, consequently, measures of genetic differentiation and kinship were negatively impacted. Our results demonstrate the importance of error evaluation during all stages of population studies, and emphasize the need to standardize procedures for microsatellite analyses. This study facilitates the application of microsatellite technology in population studies by examining common sources of genotyping error, identifying unreported problems with microsatellites, and offering solutions to prevent error and bias in population studies.

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Characterization of microsatellite loci in caribou Rangifer tarandus, and their use in other artiodactyls

Cited by 213 publications

References 7 publications

Female Philopatry and Extreme Spatial Genetic Heterogeneity in White-Tailed Deer

Female Philopatry and Extreme Spatial Genetic Heterogeneity in White-Tailed Deer

Comparative woodland caribou population surveys in Slate Islands Provincial Park, Ontario

Microsatellites behaving badly: empirical evaluation of genotyping errors and subsequent impacts on population studies

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