Characterization of microsatellite loci in the Blue‐and‐gold Macaw, <i>Ara ararauna</i> (Psittaciformes: Aves)

Caparroz, Renato; Miyaki, Cristina Yumi; Baker, Allan J.

doi:10.1046/j.1471-8286.2003.00475.x

Cited by 22 publications

(28 citation statements)

References 5 publications

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“…Similar results have been obtained for Amazona guildingii (Russello et al, 2001) and Ara ararauna (Caparroz et al, 2003). Microsatellites primers were developed for these species and were tested for cross amplification in other psitacid species.…”

Section: Microsatellitessupporting

confidence: 71%

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Molecular markers for population genetic analyses in the family Psittacidae (Psittaciformes, Aves)

Faria

Miyaki

2006

Genet. Mol. Biol.

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The selection of molecular markers for population studies is an important tool for biodiversity conservation. The family Psittacidae contains many endangered and vulnerable species and we tested three kinds of molecular markers for their potential use in population studies of five psitacid species: 43 hyacinth macaws (Anodorhynchus hyacinthinus), 42 blue-and-yellow macaws (Ara ararauna), 23 red-and-green macaws (Ara chloroptera), 19 red-spectacled amazons (Amazona pretrei); and 18 red-tailed amazons (Amazona brasiliensis). We tested 21 clones from a genomic library of golden conure (Guarouba guarouba) minisatellites and 12 pairs of microsatellite primers developed for the domestic chicken (Gallus gallus) and A. hyacinthinus. We also tested seven tetranucleotide repeat primers for their ability to amplify regions between microsatellite loci (inter simple sequence repeats, ISSRs). We were able to select seven markers that were variable in different degrees for three species (A. hyacinthinus, A. chloroptera and A. ararauna). The mini and microsatellites produced more polymorphic patterns than the ISSRs. The genetic variability of the species studied seems to be correlated with their endangered status.

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Section: Microsatellitessupporting

confidence: 71%

“…Nine microsatellite loci were developed for A. guildingii and only two resulted in successful amplification in Ara chloroptera and five in A. ararauna (Russello et al, 2001). While six primers were developed for A. ararauna and three failed to amplify any product from samples of species from the genus Amazona (Caparroz et al, 2003).…”

Section: Microsatellitesmentioning

confidence: 99%

Molecular markers for population genetic analyses in the family Psittacidae (Psittaciformes, Aves)

Faria

Miyaki

2006

Genet. Mol. Biol.

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“…Extracted DNA was used to amplify seven microsatellite loci originally developed by Caparroz et al (2003) and Russello et al (2001Russello et al ( , 2005, using primers redesigned by Gebhardt and Waits (2008) to improve amplification success when working with non-invasive samples. PCR reactions followed published protocols using the Multiplex PCR Kit (Qiagen), with exception of AgGT19 being amplified in singleplex due to problems during multiplex PCR runs.…”

Section: Microsatellite Genotyping and Data Analysismentioning

confidence: 99%

Genetic patterns and conservation of the Scarlet Macaw (Ara macao) in Costa Rica

et al. 2015

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Once widely distributed throughout the lowland forests of Costa Rica, scarlet macaws (Ara macao) have been reduced to two major, geographically separated, populations along the Pacific slope. Past demographic declines raise conservation concerns regarding the detrimental effects of population fragmentation. This investigation aimed to evaluate the current status of scarlet macaws along the Pacific slope by examining levels of genetic variation and patterns of genetic structure within and among remnant populations. Statistical analyses using multilocus genotypes revealed strong differentiation between Central and South Pacific populations, suggesting local geographic barriers have historically restricted gene flow between these localities. High genetic diversity suggests neither population suffers from genetic erosion, likely resulting from relatively large population sizes and high dispersal capacity and longevity. However, evidence of disequilibrium within the Central Pacific population infers anthropogenic threats have disrupted natural population dynamics. These results advocate on focusing available resources on habitat restoration and nest protection, as a means to assist in reestablishing demographic stability and maintain the genetic health of wild scarlet macaws in Costa Rica.

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“…Removal of loci Cfor1415, Cfor1617, and Cfor3031 in our assignment test changed the outcome more than removal of other loci (data not shown), suggesting that more robust assignments might be achieved by using more informative loci. This very desirable objective is limited in parrots by the technical difficulties inherent in isolating a large number of microsatellite loci from the taxa (Hughes et al 1998;Robertson et al 2000;Russello et al 2001;Caparroz et al 2003;Sainsbury et al 2004). This may due to a generally low abundance of microsatellites in birds (Primmer et al 1997).…”

Section: Number Of Loci and Power Of Assignment Using Microsatellite mentioning

confidence: 99%

Genetic analysis of interspecific hybridisation in the world’s only Forbes’ parakeet (Cyanoramphus forbesi) natural population

et al. 2006

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Genetic introgression from Chatham Island Red-crowned parakeet (Cyanoramphus novaezelandiae chathamensis) is a long-standing problem in the conservation of the rare Forbes' parakeet (C. forbesi) on Mangere Island, New Zealand. Microsatellite genotypes, mitochondrial DNA sequences, and morphological markers have been applied to study the situation. An extensive history of hybridisation was detected in the single remaining Forbes' parakeet population, where no less than 81% of all birds screened are considered hybrids. Genetic markers showed that a large proportion of birds identified as Forbes' parakeets by crown plumage are cryptic hybrids. Only a small proportion of birds with Forbes' parakeet morphotype were found to be genetically distinct from Chatham Island Red-crowned parakeets using microsatellite and mitochondrial DNA assignment tests, but these still represent an Evolutionarily Significant Unit (ESU) for conservation.

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Characterization of microsatellite loci in the Blue‐and‐gold Macaw, Ara ararauna (Psittaciformes: Aves)

Cited by 22 publications

References 5 publications

Molecular markers for population genetic analyses in the family Psittacidae (Psittaciformes, Aves)

Molecular markers for population genetic analyses in the family Psittacidae (Psittaciformes, Aves)

Genetic patterns and conservation of the Scarlet Macaw (Ara macao) in Costa Rica

Genetic analysis of interspecific hybridisation in the world’s only Forbes’ parakeet (Cyanoramphus forbesi) natural population

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