Characterization of microsatellite loci in the aphid species <i>Aphis gossypii </i>Glover

Vanlerberghe‐Masutti, Flavie; Chavigny, Pascal; Fuller, Susan

doi:10.1046/j.1365-294x.1999.00876.x

Cited by 48 publications

(55 citation statements)

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“…The genetic diversity found in A . gossypii through the use of molecular markers has shown that the clonal diversity is structured by its host plant 34, 35 . In the present study, A .…”

Section: Discussionmentioning

confidence: 99%

Genetic variation and phylogeographic structure of the cotton aphid, Aphis gossypii, based on mitochondrial DNA and microsatellite markers

Wang

Yang

et al. 2017

Sci Rep

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Aphis gossypii, one of the most important agricultural pests in the world, can cause serious economic losses in the main crop-producing areas. To clarify issues such as the genetic differentiation, genetic structure, and demographic history of A. gossypii populations, we used 10 nuclear microsatellite loci (SSR) and two mitochondrial gene sequences (COI and Cytb) to investigate genetic diversity and population structure of A. gossypii populations that were collected from 33 sampling sites in China from different climatic zones. SSR and mtDNA data suggested low to moderate levels of genetic diversity. A star-shaped network of mtDNA haplotypes indicated that the maternal ancestor of China cotton aphids likely originated in Xinjiang. The POPTREE, STRUCTURE and principal coordinate analysis (PCoA) revealed two genetic clusters: an eastern and a western region group. Isolation by distance (IBD) results showed a positive correlation between geographic distance and genetic distance in the vast eastern region but not in the western region. Neutrality testing and mismatch distribution analysis provided strong evidence for a recent rapid expansion in most populations. Genetic bottleneck was not detected in A. gossypii populations of China. The present work can help us to develop strategies for managing this pest.

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“…The genetic diversity found in A . gossypii through the use of molecular markers has shown that the clonal diversity is structured by its host plant 34, 35 . In the present study, A .…”

Section: Discussionmentioning

confidence: 99%

Genetic variation and phylogeographic structure of the cotton aphid, Aphis gossypii, based on mitochondrial DNA and microsatellite markers

Wang

Yang

et al. 2017

Sci Rep

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“…Adult female aphids collected from the five host plants were genotyped based on the seven microsatellite loci Ago24, Ago53, Ago59, Ago66, Ago69, Ago84, and Ago89 (40). The 106 aphids collected in the wild belonged to just four genotypes (genotypes I to IV) ( Table 2), and the genetic similarity of these four genotypes was 28 to 44% based on these seven loci.…”

Section: Methodsmentioning

confidence: 99%

Host Plant Determines the Population Size of an Obligate Symbiont (Buchnera aphidicola) in Aphids

Zhang

Cao

Zhong

et al. 2016

Appl Environ Microbiol

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bBuchnera aphidicola is an obligate endosymbiont that provides aphids with several essential nutrients. Though much is known about aphid-Buchnera interactions, the effect of the host plant on Buchnera population size remains unclear. Here we used quantitative PCR (qPCR) techniques to explore the effects of the host plant on Buchnera densities in the cotton-melon aphid, Aphis gossypii. Buchnera titers were significantly higher in populations that had been reared on cucumber for over 10 years than in populations maintained on cotton for a similar length of time. Aphids collected in the wild from hibiscus and zucchini harbored more Buchnera symbionts than those collected from cucumber and cotton. The effect of aphid genotype on the population size of Buchnera depended on the host plant upon which they fed. When aphids from populations maintained on cucumber or cotton were transferred to novel host plants, host survival and Buchnera population size fluctuated markedly for the first two generations before becoming relatively stable in the third and later generations. Host plant extracts from cucumber, pumpkin, zucchini, and cowpea added to artificial diets led to a significant increase in Buchnera titers in the aphids from the population reared on cotton, while plant extracts from cotton and zucchini led to a decrease in Buchnera titers in the aphids reared on cucumber. Gossypol, a secondary metabolite from cotton, suppressed Buchnera populations in populations from both cotton and cucumber, while cucurbitacin from cucurbit plants led to higher densities. Together, the results suggest that host plants influence Buchnera population processes and that this may provide phenotypic plasticity in host plant use for clonal aphids.A phids harbor two types of bacterial endosymbionts: the obligate symbiont Buchnera aphidicola, which occurs in almost all aphids, and facultative bacteria belonging to a variety of taxa that are not essential for aphid survival and typically are found in some but not all individuals of a population (1-3). The association between Buchnera and aphids was established 80 to 150 million years ago (4), and because transmission is strictly maternal, bacteria and host phylogenies are perfectly congruent. Buchnera is essential for the aphid, and if the symbiont is experimentally removed, the host grows very slowly and cannot reproduce (5-7). Buchnera synthesizes essential amino acids and other substances that are absent in the aphid's phloem diet (1,(8)(9)(10)(11)(12)(13) and is active in purine metabolism (14, 15). The symbiont's activities can be disrupted by heat shock, and it has been shown that variation in the promoter of a Buchnera heat shock gene impacts aphid thermal tolerance (16,17). The genome of Buchnera is much smaller than those of its free-living relatives (18, 19) and in many ways it now resembles an organelle, highly integrated within the physiology of its host.Densities of Buchnera (as measured by quantitative PCR [qPCR] relative to the host) are affected by host age and genotype and by ...

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“…The DNA was diluted 10-fold and stored at -20°C. PCR was performed using 10 μL of the reaction mixture, containing 6 μL of 2× PCR Master Mix (Jena BioScience GmbH, Jena, Germany), 1 μL (10 pM) of each forward and reverse primer (Ago89, Ago66, Ago24, Ago59, Ago53, Ago84, Ago69, Ago126) (VanlerbergheMasutti et al, 1999) and 1.5 μL of aphid template DNA (approximately 10 ng), using the following cycling parameters: initial denaturation at 94°C for 5 min followed by 35 cycles of denaturation at 94°C for 1 min, locus-specifi c annealing temperature 1 min according to Vanlerberghe-Masutti et al (1999); and extension at 74°C for 30 s, with a fi nal extension at 74°C for 5 min. Products were separated on 6% polyacrylamide urea gel at 75 W constant power (Bio-Rad Laboratories, Hercules, CA, USA).…”

Section: Dna Extraction and Microsatellite Analysismentioning

confidence: 99%

Genetic structure of populations of Aphis gossypii (Hemiptera: Aphididae) on citrus trees in Northern Iran

Gholamian¹,

Razmjou²,

Hashemian³

et al. 2018

Eur. J. Entomol.

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Characterization of microsatellite loci in the aphid species Aphis gossypii Glover

Cited by 48 publications

References 1 publication

Genetic variation and phylogeographic structure of the cotton aphid, Aphis gossypii, based on mitochondrial DNA and microsatellite markers

Genetic variation and phylogeographic structure of the cotton aphid, Aphis gossypii, based on mitochondrial DNA and microsatellite markers

Host Plant Determines the Population Size of an Obligate Symbiont (Buchnera aphidicola) in Aphids

Genetic structure of populations of Aphis gossypii (Hemiptera: Aphididae) on citrus trees in Northern Iran

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