Characterization of microsatellite loci for three species of Tomoplagia (Diptera: Tephritidae) and absence of cross-species amplification

Leal, Camila Rabelo Oliveira; Jorge, Leonardo Ré; Souza, Anete Pereira de; Lewinsohn, Thomas M.; Silva-Brandão, Karina Lucas

doi:10.1007/s13355-020-00706-6

Cited by 2 publications

(2 citation statements)

References 28 publications

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“…Individuals belonging to the three species of Tomoplagia were genotyped for 12 nuclear microsatellite loci, obtained from libraries built specifically for each species. These were amplified by polymerase chain reaction (PCR) in accordance with previously described protocols (Leal et al, 2020). The direct primers used to amplify each locus were synthesized with the addition of an M13 tail (TGTAAAACGACGGCCAGT) to the 5 0 end, for annealing the fluorophores FAM, VIC, PET, and NED (Life Technologies, Carlsbad, CA, USA) during PCR amplifications (Schuelke, 2000).…”

Section: Amplification and Genotyping Of Microsatellite Locimentioning

confidence: 99%

Host specialization, genetic variability, and differentiation in three species of Tomoplagia from Brazilian rupestrian grasslands

Leal

Souza

Silva-Brandão

et al. 2021

Entomologia Exp Applicata

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In this study, the geographic and host plant-associated genetic structure of Tomoplagia spp. (Diptera: Tephritidae) was investigated. The study was developed in the rupestrian montane grasslands of the Espinhac ßo Mountain Range, Brazil, where these flies' larvae develop within flower heads of Asteraceae. The three species of Tomoplagia investigated have different host spans within the subtribe Vernoniinae: one is strictly monophagous (Tomoplagia grandis Prado, Norrbom & Lewinsohn) and two are oligophagous [Tomoplagia incompleta (Williston) and Tomoplagia bicolor Prado, Norrbom & Lewinsohn]. The genetic variability and differentiation throughout their geographic distribution were investigated at the local scale (sympatric, between different host species in the same locality) and the regional scale (allopatric, on the same or different host species in different localities). Allele richness was greater for populations of the two oligophagous species. Moreover, population genetic structure was low in all species both on the local and regional scales, and the attribution of individuals of the three species to different genetic groups was not strongly associated with either locality or host plant. These results indicate the absence of simple geographic and host-associated genetic structure in these specialized herbivores, suggesting that females of T. bicolor and T. incompleta have low host philopatry, using various host species indiscriminately within their distribution range. Therefore, the use of host plants by fruit flies of the genus Tomoplagia is a plastic character that can shift temporally and spatially among phylogenetically related and chemically similar plants. Contrary to expectations, given the specialized and intimate nature of their host association, the studied Tomoplagia species showed no clear host-associated genetic differentiation; instead, genetic variation presents a mosaic pattern across hosts and localities.

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Section: Amplification and Genotyping Of Microsatellite Locimentioning

confidence: 99%

Host specialization, genetic variability, and differentiation in three species of Tomoplagia from Brazilian rupestrian grasslands

Leal

Souza

Silva-Brandão

et al. 2021

Entomologia Exp Applicata

Self Cite

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“…Microsatellite markers have been developed and used for many fruit fly species (Augustinos et al 2008;Bonizzoni et al 2001;Buahom et al 2013;Leal et al 2021;Lin et al 2020;Qin et al 2018;Wang et al 2021;Yan et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Development of microsatellite markers for the Japanese orange fly, Bactrocera tsuneonis (Diptera: Tephritidae)

et al. 2022

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To monitor and control the Japanese orange fly Bactrocera tsuneonis, it is important to determine their dispersal ability. Therefore, to determine the genetic structure of this species, we developed microsatellite markers at 17 loci for B. tsuneonis. A total of 80 adult females and males randomly collected from abandoned orchards in the Oita and Yamaguchi Prefectures, in the western part of Japan, were used for polymorphism analysis using the microsatellite markers. The average number of alleles was 2.65 for the 17 loci, in which two loci did not show polymorphism. The expected heterozygosity ranged from 0 to 0.663, although the observed heterozygosity was relatively low between 0-0.105. The null allele frequencies varied among populations. Moreover, no polymorphism was found in the three larval populations collected in the Oita Prefecture. These results suggested a high degree of inbreeding for this species. Significant genetic differentiation between females and males of the same population was not observed, although it was observed between the Oita and Yamaguchi populations.A bottleneck effect was detected in Yamaguchi but not in Oita, and this is congruent with the history of an expanding distribution in this species. These markers could be used for population discrimination and to expand the geographic history of this species, potentially contributing to the pest management of B. tsuneonis.

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Characterization of microsatellite loci for three species of Tomoplagia (Diptera: Tephritidae) and absence of cross-species amplification

Cited by 2 publications

References 28 publications

Host specialization, genetic variability, and differentiation in three species of Tomoplagia from Brazilian rupestrian grasslands

Host specialization, genetic variability, and differentiation in three species of Tomoplagia from Brazilian rupestrian grasslands

Development of microsatellite markers for the Japanese orange fly, Bactrocera tsuneonis (Diptera: Tephritidae)

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