Characterization of methicillin-resistant Staphylococcus aureus isolated from tertiary care hospitals in Tokyo, Japan

Nakaminami, Hidemasa; Noguchi, Norihisa; Ito, Ayumu; Ikeda, Masashi; Utsumi, Kenta; Maruyama, Hiroshi; Sakamoto, Haruo; Senoo, Masako; Takasato, Yoshio; Nishinarita, Susumu

doi:10.1016/j.jiac.2014.03.006

Cited by 37 publications

(19 citation statements)

References 13 publications

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“…MRSA in horses in North America often belongs to ST8/IV (Weese and others 2005, Lin and others 2011) and in Europe ST398/IV is a dominant equine clone (Abdelbary and others 2014, Loncaric and others 2014), but to the author's knowledge ST5/II MRSA has not been described in horses until now. In people, ST5/II is an epidemic clone, particularly prevalent in hospitals in Japan, sometimes referred to as the New York/Japan clone (Kawaguchiya and others 2013, Chen and Huang 2014, Nakaminami and others 2014, Khokhlova and others 2015). This clone causes a variety of diseases in humans, such as keratitis (Hayashi and others 2014), which has now also been described in equine cases by Kuroda and others (2016).…”

mentioning

confidence: 99%

MRSA transmission between horses and vets: who's doing the infecting?

Koop

2016

Veterinary Record

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mentioning

confidence: 99%

MRSA transmission between horses and vets: who's doing the infecting?

Koop

2016

Veterinary Record

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“…ST5‐MRSA strains carrying Type II SCC mec (ST5‐MRSA‐II) are globally disseminated around the world, including Asia, Europe, and United States . A new variant of ST5‐MRSA‐II, called ST764‐MRSA‐II, was isolated from necrotizing fasciitis, keratitis, the nasal cavity, and urine in Japan . The isolation frequency of ST764‐MRSA‐II has increased in recent years in long‐term care facilities and hospitals .…”

Section: The Psm‐mec Mutations and Their Correspondence To Sccmec Typmentioning

confidence: 99%

Epidemiological study on the relationship between toxin production and psm‐mec mutations in MRSA isolates in Thailand

Tabuchi

Lulitanond

et al. 2020

Microbiology and Immunology

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In this present study, we investigated the phenol‐soluble modulin (psm‐mec) mutations, the staphylococcal cassette chromosome mec (SCCmec) types, and toxin production in 102 methicillin‐resistant Staphylococcus aureus (MRSA) isolates from the northeast and central regions of Thailand. The MRSA isolates carrying ‐7T>C psm‐mec in Type II SCCmec (n = 18) and the MRSA isolates carrying no psm‐mec in Type IV (n = 8) or Type IX SCCmec (n = 4) had higher hemolytic activity against sheep erythrocytes than MRSA isolates carrying intact psm‐mec in Type III SCCmec (n = 34), but MRSA isolates carrying no psm‐mec in Type I SCCmec (n = 27) did not.

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“…8,19) Antimicrobial Susceptibility Testing Minimum inhibitory concentrations (MICs) were determined by an agar doubling-dilution method, according to the Clinical and Laboratory Standards Institute guidelines. 20) The following antimicrobial agents were used: ampicillin (Wako), oxacillin (Sigma-Aldrich), cefotaxime (Wako), cefdinir (Sigma-Aldrich), levofloxacin (Wako), clarithromycin (Wako), spiramycin (Sigma-Aldrich), gentamicin (Wako), minocycline (SigmaAldrich), vancomycin (Wako), fusidic acid (Sigma-Aldrich), benzalkonium chloride (Wako), and chlorhexidine digluconate (Wako).…”

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confidence: 99%

“…19) Isolates exhibiting 100% similarity were determined to be the same pulsotypes. 21) Statistical Analysis Differences in the average number of bacteria between hospital workers and students were compared using Student's t-test.…”

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confidence: 99%

Methicillin-Resistant <i>Staphylococcus epidermidis</i> Is Part of the Skin Flora on the Hands of Both Healthy Individuals and Hospital Workers

Watanabe¹,

Nakaminami

Azuma

et al. 2016

Biological & Pharmaceutical Bulletin

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Staphylococcus epidermidis, a major skin flora on hands, acts as a reservoir of various antimicrobial resistance determinants including staphylococcal cassette chromosome mec (SCCmec) and contributes to multidrug resistance for S. aureus. The aim of this study was understanding the characteristics of commensal S. epidermidis on the hands of hospital workers and healthy individuals. A total of 23 hospital workers (physicians, nurses, and hospital pharmacists), 13 community pharmacists, and 24 healthy individuals (students) were studied. Commensal bacteria on hands were recovered using a glove-juice method. For methicillinresistant S. epidermidis (MRSE), we performed SCCmec typing, pulsed-field gel electrophoresis (PFGE), and determined the antimicrobial susceptibility. The detection rates of MRSE in community pharmacists (92.3%) and students (87.5%) were higher than those in hospital workers (66.7 to 81.8%). SCCmec type IV strains were predominant in both hospital workers and students. PFGE analysis strongly suggested that the MRSE of hospital workers and students were normal inhabitants of each subject. The antimicrobial resistance rates and levels in MRSE of hospital workers were higher than those of students. Our findings showed that MRSE was frequently colonized on the hands of healthy individuals as well as hospital workers.

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Characterization of methicillin-resistant Staphylococcus aureus isolated from tertiary care hospitals in Tokyo, Japan

Cited by 37 publications

References 13 publications

MRSA transmission between horses and vets: who's doing the infecting?

MRSA transmission between horses and vets: who's doing the infecting?

Epidemiological study on the relationship between toxin production and psm‐mec mutations in MRSA isolates in Thailand

Methicillin-Resistant <i>Staphylococcus epidermidis</i> Is Part of the Skin Flora on the Hands of Both Healthy Individuals and Hospital Workers

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