Characterization of methicillin-resistant<i>Staphylococcus aureus</i>from healthy carrier chickens

Nemeghaire, Stéphanie; Roelandt, Sophie; Argudín, Maria Ángeles; Haesebrouck, Freddy; Butaye, Patrick

doi:10.1080/03079457.2013.805183

Cited by 39 publications

(37 citation statements)

References 32 publications

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“…These isolates have probably been lost during the second enrichment step since this includes antimicrobials. However, as shown for samples from poultry [19], representing a low prevalence population, the second enrichment method does not make any statistical difference. Therefore we recommend for future European surveillances to use the SBEM on nasal swabs.…”

Section: Discussionmentioning

confidence: 99%

“…MRSA ST239 spa type t388 and t037 are widespread HA-MRSA found in Europe, Asia and America [40]. A MRSA ST239 t037 was also isolated from poultry in 2011 [19]. The recovery of these HA-MRSA from livestock indicates that one should remain vigilant to the evolution of MRSA in animals.…”

Section: Discussionmentioning

confidence: 99%

“…Livestock associated (LA)-MRSA was first described in pigs in 2005 and humans in close contact with pigs in the Nederland [12] and in France [13]. This particular clone belonging to the clonal complex (CC)398 was later encountered in many healthy animals such as pigs [14], horses [15], bovines [16] and poultry [17-19]. This clonal complex is composed of different closely related spa types [20] and cannot be typed by pulsed field gel electrophoresis using SmaI digestion [21].…”

Section: Introductionmentioning

confidence: 99%

“…The European Food Safety Authority (EFSA) [22] has proposed a standardized protocol for the isolation of MRSA from dust samples obtained from pig farms. However, this protocol was estimated not to be very sensitive in a study in poultry in 2011 [19]. …”

Section: Introductionmentioning

confidence: 99%

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Epidemiology and molecular characterization of methicillin-resistant Staphylococcus aureus nasal carriage isolates from bovines

et al. 2014

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BackgroundStaphylococcus aureus is a common bacterium usually found on skin and mucous membranes of warm blooded animals. Resistance in S. aureus has been increasingly reported though depending on the clonal lineage. Indeed, while hospital acquired (HA)-methicillin resistant S. aureus (MRSA) are typically multi-resistant, community associated (CA)-MRSA are by large more susceptible to many antibiotics. Although S. aureus isolated from animals are often susceptible to most antibiotics, multi-resistant livestock associated (LA)-MRSA have been recovered from bovine mastitis.In this study, we investigated the prevalence and types of MRSA present in the nose of healthy bovines of different age groups and rearing practices. Since no validated methods for MRSA isolation from nasal swabs were available, we compared two isolation methods. Molecular characterization was performed by means of spa-typing, MLST, SCCmec typing and microarray analysis for the detection of antimicrobial resistance and virulence genes.ResultsMRSA between herd prevalence in bovines was estimated at 19.8%. There was a marked difference between rearing practices with 9.9%, 10.2% and 46.1% of the dairy, beef and veal calve farms respectively being MRSA positive. No significant difference was observed between both isolation methods tested. Most isolates were ST398 spa type t011 or closely related spa types. Few ST239 spa type t037 and t388 and ST8 spa type t121 were also found. SCCmec types carried by these strains were mainly type IV(2B), IV(2B&5) and type V. Type III and non-typeable SCCmec were recovered to a lesser extent. All isolates were multi-resistant to at least two antimicrobials in addition to the expected cefoxitin and penicillin resistance, with an average of resistance to 9.5 different antimicrobials. Isolates selected for microarray analysis carried a broad range of antimicrobial resistance and virulence genes.ConclusionMRSA were mainly present in veal farms, compared to the lower prevalence in dairy or beef farms. Multi-resistance in these strains was high. Though mainly CC398 spa t011 was found, the genetic diversity was higher than what was found for pigs in Belgium. CC8 strains, a typically human lineage but also recently found also in association with bovines, has been retrieved here also.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Epidemiology and molecular characterization of methicillin-resistant Staphylococcus aureus nasal carriage isolates from bovines

et al. 2014

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“…Staphylococcus aureus is a prominent cause of human infections worldwide (1-3) that causes a broad spectrum of diseases. This organism has the remarkable ability to acquire antibiotic resistance determinants and now with the emergence of multi-drug resistant methicillin-resistant S. aureus (MRSA) isolates; it has become a warning sign for public health (4).…”

Section: Introductionmentioning

confidence: 99%

Microbial Susceptibility and Plasmid Profiles of Methicillin-Resistant Staphylococcus aureus and Methicillin-Susceptible S. aureus

Fatemeh

Rashki

Ghalehnoo

2014

Jundishapur J Microbiol

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Background:Today, significant increase in the prevalence and emergence of methicillin-resistant Staphylococcus aureus (MRSA) is a serious public health concern and is likely to have a dramatic negative impact on many current medical practices. Therefore, identification of MRSA strains is important for both clinical and epidemiological implications.Objectives:The present study was carried out to determine the frequency of methicillin resistant; antibiotic susceptibility and plasmid profiles of S. aureus recovered from different types of clinical samples of patients in Zabol, Iran.Material and Methods:Clinical samples from 500 outpatient and hospitalized patients were tested for S. aureus. The susceptibility of 106 S. aureus to 11 antibiotics was evaluated by the disk diffusion method and Etest oxacillin strips. The presence of mecA gene was investigated by polymerase chain reaction (PCR). The plasmid profile patterns of all isolates were determined by a modified alkaline lysis method.Results:A total of 67 (63.20%) strains were found to be MRSA isolates. Most of MRSA isolates showed high level of resistance to ampicillin, erythromycin, nalidixic acid, penicillin, and tetracycline. Twenty-six percent of MRSA isolates showed high level of resistance to oxacillin (minimum inhibitory concentration [MIC] ≥ 256 μg/mL). mecA gene was detected among 62 MRSA isolates. Totally, 75 isolates of both strains harbored plasmid.Conclusions:Resistance to oxacillin and other antibiotics was high, and most of the isolates were found to be multi-drug resistance (MDR). Plasmid analysis of representative S. aureus isolates also demonstrates the presence of a wide range of plasmid sizes, with no consistent relationship between plasmid profiles and resistance phenotypes. Regular surveillance of hospital infections and monitoring of their antibiotic sensitivity patterns are required to reduce MRSA prevalence. High prevalence and multi-drug resistance of MRSA isolates in southeast of Iran could be considered as an urgent warning for public health.

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Other Bacterial Diseases

Logue¹,

Andreasen²,

Borst³

et al. 2019

Diseases of Poultry

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Characterization of methicillin-resistantStaphylococcus aureusfrom healthy carrier chickens

Cited by 39 publications

References 32 publications

Epidemiology and molecular characterization of methicillin-resistant Staphylococcus aureus nasal carriage isolates from bovines

Epidemiology and molecular characterization of methicillin-resistant Staphylococcus aureus nasal carriage isolates from bovines

Microbial Susceptibility and Plasmid Profiles of Methicillin-Resistant Staphylococcus aureus and Methicillin-Susceptible S. aureus

Other Bacterial Diseases

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