Characterization of metabolomic differences in peanut worm Sipunculus nudus between breeding and nonbreeding seasons

Yang, Chuangye; Zhang, Jiawei; Zhong, Ruzhuo; Guo, Zhicheng; Wang, Qingheng; Zheng, Zhe

doi:10.1016/j.aqrep.2019.100271

Cited by 6 publications

(9 citation statements)

References 35 publications

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“…After the 90-day culture period, eight S. nudus from each group were randomly selected and coelomic fluid from juvenile S. nudus was extracted following the methods of Yang et al (2020), immediately placed in liquid nitrogen, and stored at -80 • C.…”

Section: Sample Collectionmentioning

confidence: 99%

“…Artificial breeding can be used to mitigate the exploitation of wild resources. Many studies have been conducted on the population genetics (Du et al, 2008(Du et al, , 2009 and reproductive biology (Wang et al, 2005;Cao et al, 2020;Yang et al, 2020; of S. nudus. Given that growth performance is greatly affected by diet quality, studies of the nutritional requirements, and artificial feed of juvenile S. nudus could aid its artificial breeding, enhance its aquaculture, and promote the restoration of wild populations.…”

Section: Introductionmentioning

confidence: 99%

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Dietary Carbohydrate and Protein Levels Affect the Growth Performance of Juvenile Peanut Worm (Sipunculus nudus): An LC–MS-Based Metabolomics Study

et al. 2021

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The peanut worm (Sipunculus nudus) is an economically important fishery resource in China. To determine how dietary carbohydrate and protein levels affect the growth performance of juvenile S. nudus and identify the mechanisms underlying observed patterns, five isoenergetic and isolipidic diets with different levels of carbohydrate and protein were formulated and fed to juvenile S. nudus; the experimental groups were referred to as EG1, EG2, EG3, EG4, and EG5, respectively. After 90 days of feeding, S. nudus had significantly lower survival rates when fed D5 compared with other diets (P < 0.05), and the highest survival rate was observed in EG2 individuals. The weight gain rate and specific growth rate were significantly higher in EG2 compared with the other groups (P < 0.05). Metabolomic profiling using liquid chromatography–mass spectrometry revealed 83 significantly differential metabolites (POS: 59; NEG: 24), which were identified via an in-house MS2 database. Pathway analysis indicated that the significantly different metabolites were involved in 22 metabolic pathways (POS: 9; NEG: 13), including tyrosine, phenylalanine, and tryptophan biosynthesis; phenylalanine metabolism; D-glutamate and D-glutamine metabolism; proline and arginine metabolism; aspartate, alanine, and glutamate metabolism; and aminoacyl-tRNA biosynthesis. These analyses implied that the biosynthetic capabilities of juvenile S. nudus were greater in the EG2. The results of this research enhance our understanding of the effects of dietary carbohydrate and protein levels on the growth performance of juvenile S. nudus.

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Section: Sample Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dietary Carbohydrate and Protein Levels Affect the Growth Performance of Juvenile Peanut Worm (Sipunculus nudus): An LC–MS-Based Metabolomics Study

et al. 2021

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“…Metabolomics has been proposed to be a model for metabolic studies ( Cappello et al, 2017 ; Cappello et al, 2018 ; Hao et al, 2018a ), to assess metabolic responses to nutritional deficiencies or excesses providing in-depth mechanistic insights to assist the development of optimized feeding regimes ( Alfaro and Young, 2018 ; Hao et al, 2018a ). Being an evolving technology and analytical tool, metabolomics is widely used to quantify low-molecular-weight metabolites using distinct tools categorized by the type of instrumental data and the functionality such as nuclear magnetic resonance (NMR), gas chromatography coupled to mass spectrometry (GC–MS), and liquid chromatography coupled with single-stage mass spectrometry (LC–MS) ( Alfaro and Young, 2018 ; Venter et al, 2018 ; Zhang et al, 2021 ; Yang et al, 2020 ; Young and Alfaro, 2018 ). Exploring this approach in evaluating impacts of limited food, nutrient being supplemented, modifications in nutrient quantities, and replacing protein or lipid diets in aquatic animals has been conducted ( Alfaro and Young, 2018 ; Yang et al, 2018b ).…”

Section: Introductionmentioning

confidence: 99%

Integrated GC–MS- and LC–MS-Based Untargeted Metabolomics Studies of the Effect of Vitamin D3 on Pearl Production Traits in Pearl Oyster Pinctada fucata martensii

Yang

Zeng

Liao

et al. 2021

Front. Mol. Biosci.

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Pearl oyster Pinctada fucata martensii is widely recognized for biomineralization and has been cultured for high-quality marine pearl production. To ascertain how dietary vitamin D3 (VD3) levels affect the features of pearl production by P. f. martensii and discover the mechanisms regulating this occurrence, five experimental diets with variable levels of VD3 were used with inclusion levels of 0, 500, 1,000, 3,000, and 10,000 IU/kg. The distinct inclusion levels were distributed into five experimental groups (EG1, EG2, EG3, EG4, and EG5). All the experimental groups were reared indoors except the control group (CG) reared at the sea. Pearl oysters, one year and a half old, were used in the grafting operation to culture pearls. During the growing period that lasted 137 days, EG3 had the highest survival rate, retention rate, and high-quality pearl rate. A similar trend was found for EG3 and CG with significantly higher pearl thickness and nacre deposition rates than other groups, but no significant differences were observed between them. A metabolomics profiling using GC–MS and LC–MS of pearl oysters fed with low quantities of dietary VD3 and optimal levels of dietary VD3 revealed 135 statistically differential metabolites (SDMs) (VIP > 1 and p < 0.05). Pathway analysis indicated that SDMs were involved in 32 pathways, such as phenylalanine metabolism, histidine metabolism, glycerophospholipid metabolism, alanine aspartate and glutamate metabolism, arginine and proline metabolism, glycerolipid metabolism, amino sugar and nucleotide sugar metabolism, and tyrosine metabolism. These results provide a theoretical foundation for understanding the impacts of VD3 on pearl production traits in pearl oyster and reinforce forthcoming prospects and application of VD3 in pearl oyster in aquaculture rearing conditions.

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“…Sipunculus nudus (S. nudus), as well as also called grid siphon worm and light naked siphon worm, are an important economic and ecological species and widely distributed in the South China Sea region [1][2]. As one of famous marine products, S. nudus possess the high nutritional composition, such as protein, mineral elements such as sodium and potassium, water-soluble and fat-soluble vitamins (i.e.VB1, VB2, and VB4), taurine, as well as essential amino acids for human body [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Determination of total free amino acids in Sipunculus nudus by UV spectrophotometry

Cai

Zhang

et al. 2020

E3S Web Conf.

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Here we developed a new, facile method to detect free amino acids (TFAA) in Sipunculus nudus by UV spectrophotometry. The pretreatment conditions of the approach were optimized, such as chromogenic reaction temperature and time, the measurement time after chromogenic reaction, the extraction agent and its concentration, the extraction time. The established method showed a good linearity in the TFAA range 0.0 ~ 40.0 cg/mL (R2= 0.9958) with the limit of detection (LOD) 1.96 g /mL and the precision of 6.14 % (relative standard deviation, RSD). The addition standard recoveries were 95.1 % ~108.6 %. Finally, the method was successfully applied in detection of TFAA in Sipunculus nudus and other aquatic products. The results showed that the content of TFAA in Sipunculus nudus was higher than that in the other aquatic products collected in our paper, and as high as 138.14 g/kg.

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Characterization of metabolomic differences in peanut worm Sipunculus nudus between breeding and nonbreeding seasons

Cited by 6 publications

References 35 publications

Dietary Carbohydrate and Protein Levels Affect the Growth Performance of Juvenile Peanut Worm (Sipunculus nudus): An LC–MS-Based Metabolomics Study

Dietary Carbohydrate and Protein Levels Affect the Growth Performance of Juvenile Peanut Worm (Sipunculus nudus): An LC–MS-Based Metabolomics Study

Integrated GC–MS- and LC–MS-Based Untargeted Metabolomics Studies of the Effect of Vitamin D3 on Pearl Production Traits in Pearl Oyster Pinctada fucata martensii

Determination of total free amino acids in Sipunculus nudus by UV spectrophotometry

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