Characterization of Metabolic Patterns in Mouse Oocytes during Meiotic Maturation

Li, Ling; Zhu, Shuai; Shu, Wenjie; Guo, Yueshuai; Guan, Yusheng; Zeng, Juan; Wang, Haichao; Han, Longsen; Zhang, Jiaqi; Liu, Xiaohui; Li, Chunling; Hou, Xiaohong; Gao, Min; Ge, Juan; Ren, Chao; Zhang, Hao; Schedl, Tim; Guo, Xuejiang; Chen, Minjian; Wang, Qiang

doi:10.1016/j.molcel.2020.09.022

Cited by 92 publications

(88 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Antibodies to digoxigenin (Anti-Dig) at a concentration of 1: 5000 do not bind to the surface and internal structures of oocytes ( Supplementary Figure S6b ). We selected a positive control, a worm’s gene homologous to the vertebrates’ gene Acox3 (Peroxisomal acyl-coenzyme A oxidase 3), involved in the degradation of fatty acids during oocyte maturation in mice [ 12 ]. According to our data, the Pdum-Acox3 is transcribed in Pdum oocytes ( Supplementary Figure S7a–c ).…”

Section: Resultsmentioning

confidence: 99%

Maternal Transcripts of Hox Genes Are Found in Oocytes of Platynereis dumerilii (Annelida, Nereididae)

Maslakov

Kulishkin

Surkova

et al. 2021

JDB

View full text Add to dashboard Cite

Hox genes are some of the best studied developmental control genes. In the overwhelming majority of bilateral animals, these genes are sequentially activated along the main body axis during the establishment of the ground plane, i.e., at the moment of gastrulation. Their activation is necessary for the correct differentiation of cell lines, but at the same time it reduces the level of stemness. That is why the chromatin of Hox loci in the pre-gastrulating embryo is in a bivalent state. It carries both repressive and permissive epigenetic markers at H3 histone residues, leading to transcriptional repression. There is a paradox that maternal RNAs, and in some cases the proteins of the Hox genes, are present in oocytes and preimplantation embryos in mammals. Their functions should be different from the zygotic ones and have not been studied to date. Our object is the errant annelid Platynereis dumerilii. This model is convenient for studying new functions and mechanisms of regulation of Hox genes, because it is incomparably simpler than laboratory vertebrates. Using a standard RT-PCR on cDNA template which was obtained by reverse transcription using random primers, we found that maternal transcripts of almost all Hox genes are present in unfertilized oocytes of worm. We assessed the localization of these transcripts using WMISH.

show abstract

Section: Resultsmentioning

confidence: 99%

Maternal Transcripts of Hox Genes Are Found in Oocytes of Platynereis dumerilii (Annelida, Nereididae)

Maslakov

Kulishkin

Surkova

et al. 2021

JDB

View full text Add to dashboard Cite

show abstract

“…Mitochondria are organelles that play a vital role in complex processes, including metabolism, signaling and programmed cell death ( Li et al, 2020 ). During oocyte maturation and fertilization and early embryo development, mitochondria provide energy through oxidative phosphorylation, which is critical for transcription and translation.…”

Section: Discussionmentioning

confidence: 99%

“…The second wave of primordial follicles that reach the antral stage require approximately 47 days in a mouse model. Mature oocyte quality alteration is associated with spindle morphology, chromosome aneuploidy and subcellular organelles distribution, such as mitochondria and the endoplasmic reticulum ( Li et al, 2020 ). Oocyte quality is closely coupled with the follicle and oocyte growth states, which determines the subsequent competency of maturation, fertilization, and embryonic development ( Dou et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Cyclophosphamide Exposure Causes Long-Term Detrimental Effect of Oocytes Developmental Competence Through Affecting the Epigenetic Modification and Maternal Factors’ Transcription During Oocyte Growth

Yang

Jin

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Cyclophosphamide (CTX) is widely used in various cancer therapies and in immunosuppression, and patients can still have babies after CTX chemotherapy. CTX directly causes primordial follicle loss with overactivation and DNA damage-induced apoptosis. Previous studies have shown that maternal exposure to CTX before conception increases the incidence of birth abnormalities and alters the methylation of genes in the oocytes of offspring. Mice were treated with a single dose of CTX (100 mg/kg) at post-natal day 21 and sacrificed 47 days later when primordial follicles surviving chemotherapy developed to the antral stage. Acute DNA damage and acceleration of the activation of primordial follicles after CTX treatment were repaired within several days, but the remaining follicle numbers remarkably decrease. Although partial surviving primordial follicle were developed to mature oocyte, oocyte quality hemostasis was impaired exhibiting aberrant meiosis progression, abnormal spindle and aneuploidy, mitochondrial dysfunction and increased endoplasmic reticulum stress. Thereafter, embryo development competency significantly decreased with fewer blastocyst formation after CTX exposure. CTX treatment resulted in alteration of DNA methylations and histone modifications in fully grown GV oocytes. Single-cell RNA-seq revealed CTX treatment suppressed multiple maternal genes’ transcription including many methyltransferases and maternal factor YAP1, which probably accounts for low quality of CTX-repaired oocyte. In vitro addition of lysophosphatidic acid (LPA) to embryo culture media to promote YAP1 nuclear localization improved CTX-repaired embryo developmental competence. This study provides evidence for the consistent toxic effect of CTX exposure during follicle development, and provide a new mechanism and new insights into future clinical interventions for fertility preservation.

show abstract

“…In mice, glucose metabolism plays a key role in promoting the nuclear and cytoplasmic maturation of oocytes in vitro (Herrick et al 2006;Xie et al 2016). Moreover, specific metabolites or metabolic pathways can generate signals that influence the initiation of meiosis (Li et al 2020). Retinoic acid is critical for meiotic initiation in mouse germ cells (Bowles et al 2006;Koubova et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…In addition to molecular genetics studies of meiotic progression, high-throughput analysis of meiosis, such as transcriptomics (Chen et al 2010;Yang et al 2011) and proteomics (Osman et al 2013), have been widely adopted in recent years. However, few studies have employed metabolomics to systematically investigate the impact in meiosis on the metabolome (Li et al 2020). Metabolomics analysis has become a powerful technology to comprehensively clarify plant development (Wen et al 2015;Zhu et al 2018) and for molecular phenotyping (Dai et al 2010a;Ren et al 2009;Garcia-Perez et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Metabolic Profiling of Arabidopsis Inflorescences Between Landsberg erecta and Columbia, and Meiosis-Defective Mutants by 1H-NMR Spectroscopy

Wang

et al. 2021

Phenomics

View full text Add to dashboard Cite

With the rapid development of omics technologies during the last several decades, genomics, transcriptomics, and proteomics have been extensively used to characterize gene or protein functions in many organisms at the cell or tissue level. However, metabolomics has not been conducted in reproductive organs, with a focus on meiosis in plants. In this study, we adopted a nuclear magnetic resonance (NMR)-based metabolomics approach to reveal the metabolic profile of inflorescences from two Arabidopsis accessions, Columbia (Col) and Landsberg erecta (Ler), and several sterile mutants caused by meiosis defects. We identified 68 dominant metabolites in the samples. Col and Ler displayed distinct metabolite profiles. Interestingly, mutants with similar meiotic defects, such as Atrad51-3, Atrfc1-2, and Atpol2a-2, exhibited similar alterations in metabolites, including upregulation of energy metabolites and promotion of compounds related to maintenance of genomic stability, cytoplasmic homeostasis, and membrane integrity. The collective data reveal distinct changes in metabolites in Arabidopsis inflorescences between the Col and Ler wild type accessions. NMR-based metabolomics could be an effective tool for molecular phenotyping in studies of aspects of plant reproductive development such as meiosis.

show abstract

Characterization of Metabolic Patterns in Mouse Oocytes during Meiotic Maturation

Cited by 92 publications

References 82 publications

Maternal Transcripts of Hox Genes Are Found in Oocytes of Platynereis dumerilii (Annelida, Nereididae)

Maternal Transcripts of Hox Genes Are Found in Oocytes of Platynereis dumerilii (Annelida, Nereididae)

Cyclophosphamide Exposure Causes Long-Term Detrimental Effect of Oocytes Developmental Competence Through Affecting the Epigenetic Modification and Maternal Factors’ Transcription During Oocyte Growth

Comparison of Metabolic Profiling of Arabidopsis Inflorescences Between Landsberg erecta and Columbia, and Meiosis-Defective Mutants by 1H-NMR Spectroscopy

Contact Info

Product

Resources

About