Characterization of meningococcal serogroup B outer membrane vesicle vaccines from strain 44/76 after growth in different media

Tsolakos, Nikos; Lie, Kristian; Bolstad, Karin; Maslen, Sarah; Kristiansen, Paul A.; Høiby, E. Arne; Wallington, Amanda; Vipond, Caroline; Skehel, J. Mark; Tang, Christoph M.; Feavers, Ian M.; Wedege, Elisabeth; Wheeler, Jun X.

doi:10.1016/j.vaccine.2010.02.023

Cited by 27 publications

(24 citation statements)

References 35 publications

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“…1A). Omp85+ OMVs expressed negligible levels of both proteins; OpaJ was modestly increased in the wt 2 OMV control, whereas a dominant OpcA band was observed in wt 1 OMVs, as previously reported [33]. These antigens might possibly affect the bactericidal activity of the OMV vaccines.…”

Section: Recombinant and Control Omv Vaccinessupporting

confidence: 80%

“…OMVs with overexpressed Omp85 were designated Omp85+ OMVs. OMVs from strain 44/76, grown in a fermentor as described above, but without IPTG, were used as a wt 1 OMV control [33]. As these two OMVs were found to differ in the content of opacity proteins (see Results), a second OMV control (wt 2 OMVs) was prepared from the transformed strain grown on chocolate agar plates without IPTG.…”

Section: Meningococcalmentioning

confidence: 99%

“…Female Balb/c and C57BL/6 mice (Taconic M&B, Ltd., Ry, Denmark) were vaccinated subcutaneously with two 2 lg doses of the OMV vaccines 3 weeks apart, and sera collected 2 weeks after the second dose. Sera from female NMRI mice, vaccinated in the same way with the wt 1 OMV vaccine, were obtained during a previous study [33]. Female OFI mice (Charles River, Lyon, France) received three 5 lg doses of the Omp85+ vaccine intramuscularly at days 0, 21 and 28 with sampling of sera 2 weeks later [16].…”

Section: Meningococcalmentioning

confidence: 99%

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Meningococcal Omp85 in Detergent‐Extracted Outer Membrane Vesicle Vaccines Induces High Levels of Non‐Functional Antibodies in Mice

et al. 2013

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The vaccine potential of meningococcal Omp85 was studied by comparing the immune responses of genetically modified deoxycholate-extracted outer membrane vesicles, expressing five-fold higher levels of Omp85, with wild-type vesicles. Groups (n = 6-12) of inbred and outbred mouse strains (Balb/c, C57BL/ 6, OFI and NMRI) were immunized with the two vaccines, and the induced antibody levels and bactericidal and opsonic activities measured. Except for Balb/ c mice, which were low responders, the genetically modified vaccine raised high Omp85 antibody levels in all mouse strains. In comparison, the wild-type vaccine gave lower antibody levels, but NMRI mice responded to this vaccine with the same high levels as the modified vaccine in the other strains. Although the vaccines induced strain-dependent Omp85 antibody responses, the mouse strains showed high and similar serum bactericidal titres. Titres were negligible with heterologous or PorA-negative meningococcal target strains, demonstrating the presence of the dominant bactericidal PorA antibodies. The two vaccines induced the same opsonic titres. Thus, the genetically modified vaccine with high Omp85 antibody levels and the wild-type vaccine induced the same levels of functional activities related to protection against meningococcal disease, suggesting that meningococcal Omp85 is a less attractive vaccine antigen.

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Section: Recombinant and Control Omv Vaccinessupporting

confidence: 80%

Section: Meningococcalmentioning

confidence: 99%

Section: Meningococcalmentioning

confidence: 99%

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Meningococcal Omp85 in Detergent‐Extracted Outer Membrane Vesicle Vaccines Induces High Levels of Non‐Functional Antibodies in Mice

et al. 2013

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“…The use of vesicles as vaccines has been extensively explored in Vibrio cholerae (30,31) and Neisseria species (10,34,40,41). In one study, female mice were immunized with OMVs from Vibrio cholerae, and their offspring were protected against subsequent challenge by V. cholerae.…”

Section: Discussionmentioning

confidence: 99%

Immunization with Salmonella enterica Serovar Typhimurium-Derived Outer Membrane Vesicles Delivering the Pneumococcal Protein PspA Confers Protection against Challenge with Streptococcus pneumoniae

et al. 2011

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Gram-negative bacteria produce outer membrane vesicles (OMVs) that serve a variety of functions related to survival and pathogenicity. Periplasmic and outer membrane proteins are naturally captured during vesicle formation. This property has been exploited as a method to derive immunogenic vesicle preparations for use as vaccines. In this work, we constructed a Salmonella enterica serovar Typhimurium strain that synthesized a derivative of the pneumococcal protein PspA engineered to be secreted into the periplasmic space. Vesicles isolated from this strain contained PspA in the lumen. Mice intranasally immunized with the vesicle preparation developed serum antibody responses against vesicle components that included PspA and Salmonelladerived lipopolysaccharide and outer membrane proteins, while no detectable responses developed in mice immunized with an equivalent dose of purified PspA. Mucosal IgA responses developed against the Salmonella components, while the response to PspA was less apparent in most mice. Mice immunized with the vesicle preparation were completely protected against a 10؋ 50% lethal dose (LD 50 ) challenge of Streptococcus pneumoniae and significantly protected against a 200؋ LD 50 challenge, while control mice immunized with purified PspA or empty vesicles were not protected. These results establish that vesicles can be used to mucosally deliver an antigen from a Gram-positive organism and induce a protective immune response.

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“…Since OMVs contain mainly outer membrane proteins, they are considered as potentially effective components of subunit vaccine against some pathogens. Most of the proteomic work has been done on N. meningitidis OMVs [41][42][43][44]. A smaller number of studies performed so far on OMVs produced by other gram negative bacteria indicate that they induce immunity and may be considered as efficient vaccine components against other infectious diseases such as cholera or pertussis [45][46][47].…”

mentioning

confidence: 99%

Proteomics for development of vaccine

Adamczyk-Popławska

Markowicz

Jagusztyn-Krynicka

2011

Journal of Proteomics

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Characterization of meningococcal serogroup B outer membrane vesicle vaccines from strain 44/76 after growth in different media

Cited by 27 publications

References 35 publications

Meningococcal Omp85 in Detergent‐Extracted Outer Membrane Vesicle Vaccines Induces High Levels of Non‐Functional Antibodies in Mice

Meningococcal Omp85 in Detergent‐Extracted Outer Membrane Vesicle Vaccines Induces High Levels of Non‐Functional Antibodies in Mice

Immunization with Salmonella enterica Serovar Typhimurium-Derived Outer Membrane Vesicles Delivering the Pneumococcal Protein PspA Confers Protection against Challenge with Streptococcus pneumoniae

Proteomics for development of vaccine

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