Characterization of marigold (Tagetes spp.) genotypes using SDS-PAGE and RAPD markers

Mor, V.S.; Deswal, Deepa; Mann, Anita; Dahiya, B. S.; Beniwal, B. S.

doi:10.15258/sst.2008.36.3.25

Cited by 7 publications

(2 citation statements)

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“…The percentage of polymorphism ranged from 80% to 100% with an average of 98.80%. The polymorphism observed here was comparatively higher than previously observed in related species like 7% in Tagetes minuta (Daroker et al 2000), 70% in Tagetes patula (Daroker et al 2000), 57.92% (Namita et al 2013) and 84% (Mor et al 2008) in marigold, 69.51% in sunflower (Isaacs et al 2003), 89.7% in Chrysanthemum morifolium (Xu et al 2006) and 83.33% in Chrysanthemum grandiflorum (Kumar et al 2014). In the present study, a wide range for the number of amplicons per primer was observed, i.e.…”

Section: Rapd Polymorphismcontrasting

confidence: 56%

Molecular characterization of African marigold (Tagetes erecta) genotypes using RAPD markers

et al. 2017

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The present investigation was carried out to characterize 22 genotypes of marigold (Tagetes erecta L.) with RAPD (randomly amplified polymorphic DNA) markers. A total of 26 RAPD markers were utilized for assessment of genetic divergence in marigold which amplified a total of 241 amplicons, out of which 238 (98.80%) were found polymorphic. The average number of amplicon per primer was 9.27. The Jaccard's similarity coefficient ranged from0.18 to 0.80. The mean values of polymorphic information content (PIC), resolving power (RP) and marker index (MI) of RAPD primers were 0.38, 8.36 and 3.44 respectively. Cluster analysis was done by unweighted pair group method for arithmetic average (UPGMA) and Principal Component analysis (PCA) was also done for the grouping of genotypes. The broad range of Jaccard's similarity coefficient observed for the genotypic data generated suggest the wide genetic base for marigold genotypes which will be effectively explored for future breeding programmes.

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Section: Rapd Polymorphismcontrasting

confidence: 56%

Molecular characterization of African marigold (Tagetes erecta) genotypes using RAPD markers

et al. 2017

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“…Breeders may wish the introduction of a specific characteristic controlled by a gene or a group of genes for a given species without significant change in the genetic material of that category containing desirable traits. In this case, the nearest species with that characteristic is selected for the species under study because it is difficult to find the genetic dimension between the based on phenotypic traits (Mor et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

The Study of Genetic Structure and Relationship of Iraqi and Syrian Cicer Arienum Using the RAPD Molecular Indicator

Al-Hasnawi¹

2020

ATMPH

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Background: Chickpeas, also known as Hummus, (Cicer arietinum L) is a self-pollinating plant. Hummus is of important nutritional value as it has good balance between proteins and carbohydrates. The aims o current study were to detect the genetic differences between DNAs of the studied species, findthe distinctive molecular DNA of the studied species and to determine the genetic relationship between the studied cultivars. Methods: The Random Amplified Polymorphic DNA analysis was performed in order to prepare the DNA of 6 types of Iraqi chickpeas and 4 types of Syrian chickpeas. Ten primers were chosen which presented contrasted multiplied results among the types included in the study. Those primers showed (107) contrasted Polymorphic bands out of (145) total bands.Results: Each selected primer produced between ( 62) packages (OPB-18) and ( 115) packages (OPB-17). The volume of multiplied products (amplicons) ranged from (100) base pairs (OPA-10) and (OPB-07) to 1800 base pairs . The largest number of heterogeneous packets (20 packets) was produced by the OPA-10 primer, while the lowest number of packets varied (5 packets) which was produced by the primer (OPC-08). The primersdiffered in their ability to find different types of polymorphic RAPD profile, where the DNA of the studied chickpeas' varieties, which reflected individual features specific to DNA, could be found.Conclusion: The lowest genetic dimension was (0.27794) between the two cultivars (1) the local cultivar (10) the local indicator, while the largest hereditary dimension was(0.27794) between the two structures (IPA505) and 7 GAB4.

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Marigold

Gupta

Panwar

Banyal

et al. 2021

Floriculture and Ornamental Plants

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Characterization of marigold (Tagetes spp.) genotypes using SDS-PAGE and RAPD markers

Cited by 7 publications

References 0 publications

Molecular characterization of African marigold (Tagetes erecta) genotypes using RAPD markers

Molecular characterization of African marigold (Tagetes erecta) genotypes using RAPD markers

The Study of Genetic Structure and Relationship of Iraqi and Syrian Cicer Arienum Using the RAPD Molecular Indicator

Marigold

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