Characterization of mammary pathogenic Escherichia coli reveals the diversity of Escherichia coli isolates associated with bovine clinical mastitis in Brazil

Orsi, Henrique; Guimarães, F. F.; Leite, Domingos S.; Guerra, Simony Trevizan; Joaquim, Sâmea Fernandes; Pantoja, J.C.F.; Hernandes, Rodrigo T.; Lucheis, Simone Baldini; Ribeiro, Márcio Garcia; Langoni, Hélio; Rall, Vera Lúcia Mores

doi:10.3168/jds.2022-22126

Cited by 12 publications

(12 citation statements)

References 59 publications

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“…The phylogenetic placement of the murine mastitis-causing E. coli isolates aligns with the core genome phylogeny. The analysis showed that G2M6U and G6M1F clustered separately, and they were more closely related to human enterotoxigenic E. coli strains from Bangladesh and India, bovine mastitis-causing strains from China (Jia 2020), France [ 81 ], and Brazil [ 82 ], as well as environmental soil-derived E. coli isolates from Bangladesh ( https://rb.gy/pa88n ). It is noteworthy that E. coli isolates assigned to MPEC phylogroups are commonly found as commensal microorganisms in the gut or in the environment [ 83 ].…”

Section: Discussionmentioning

confidence: 99%

Unveiling distinct genetic features in multidrug-resistant Escherichia coli isolated from mammary tissue and gut of mastitis induced mice

Hoque,

Faisal,

Jerin

et al. 2024

Heliyon

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Section: Discussionmentioning

confidence: 99%

Unveiling distinct genetic features in multidrug-resistant Escherichia coli isolated from mammary tissue and gut of mastitis induced mice

Hoque,

Faisal,

Jerin

et al. 2024

Heliyon

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“…The ELISA was first developed as a modification of RIA, and two research teams invented a direct form of ELISA simultaneously, named Eva Engvall and Peter Perlman, and Van Weemen and Schuurs. They devised it by tagging antigen and antibody radioisotopes in RIA with the help of enzymes, rather than radioactive iodine [ 125 ]. This method was employed to determine the level of IgG in the serum of rabbits [ 181 ].…”

Section: Advances In Diagnostic Approachesmentioning

confidence: 99%

Advances in Diagnostic Approaches and Therapeutic Management in Bovine Mastitis

Kour

Sharma

Balaji

et al. 2023

Veterinary Sciences

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Mastitis causes huge economic losses to dairy farmers worldwide, which largely negatively affects the quality and quantity of milk. Mastitis decreases overall milk production, degrades milk quality, increases milk losses because of milk being discarded, and increases overall production costs due to higher treatment and labour costs and premature culling. This review article discusses mastitis with respect to its clinical epidemiology, the pathogens involved, economic losses, and basic and advanced diagnostic tools that have been used in recent times to diagnose mastitis effectively. There is an increasing focus on the application of novel therapeutic approaches as an alternative to conventional antibiotic therapy because of the decreasing effectiveness of antibiotics, emergence of antibiotic-resistant bacteria, issue of antibiotic residues in the food chain, food safety issues, and environmental impacts. This article also discussed nanoparticles’/chitosan’s roles in antibiotic-resistant strains and ethno-veterinary practices for mastitis treatment in dairy cattle.

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“…Escherichia coli ( E. coli ) is one of the main causative agents of clinical mammary mastitis [ 1 – 3 ], which results in enormous economic losses to the dairy industry worldwide. Mammary pathogenic Escherichia coli (MPEC) represents a subset of extraintestinal pathogenic E. coli strains [ 4 ]. These bacteria exhibit an affinity for adhering to and infecting epithelial cells within the mammary glands of dairy cows alongside other pathogens, culminating in the eventual formation of biofilms [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Role of LsrR in the regulation of biofilm formation in mammary pathogenic Escherichia coli

Xu,

Wang,

Zhang

et al. 2024

BMC Vet Res

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Background Mammary Pathogenic Escherichia coli (MPEC) is an important pathogen that can escape the attack of the host immune system through biofilm formation and proliferate in the mammary gland continuously, resulting in mastitis in cows and causing enormous economic losses. As an effector of AI-2 quorum sensing, LsrR extensively affects the expression levels of hundreds of genes related to multiple biological processes in model E. coli strain. However, the regulatory role of LsrR in MPEC and whether it is involved in pathogenesis has been seldom reported. Results In this study, the function of LsrR in strain MPEC5, obtained from a milk sample in dairy cows with mastitis, was investigated by performing high-throughput sequencing (RNA-seq) assays. The results revealed that LsrR down-regulated the transcript levels of fimAICDFGH (encoding Type 1 pili), which have been reported to be associated with biofilm formation process. Biofilm assays confirmed that deletion of lsrR resulted in a significant increase in biofilm formation in vitro. In addition, electrophoretic mobility shift assay (EMSA) provided evidence that LsrR protein could directly bind to the promoter regions of fimAICDFGH in a dose-dependent manner. Conclusions These results indicate that LsrR protein inhibits the biofilm formation ability of MPEC5 by directly binding to the fimAICDFGH promoter region. This study presents a novel clue for further exploration of the prevention and treatment of MPEC.

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Characterization of mammary pathogenic Escherichia coli reveals the diversity of Escherichia coli isolates associated with bovine clinical mastitis in Brazil

Cited by 12 publications

References 59 publications

Unveiling distinct genetic features in multidrug-resistant Escherichia coli isolated from mammary tissue and gut of mastitis induced mice

Unveiling distinct genetic features in multidrug-resistant Escherichia coli isolated from mammary tissue and gut of mastitis induced mice

Advances in Diagnostic Approaches and Therapeutic Management in Bovine Mastitis

Role of LsrR in the regulation of biofilm formation in mammary pathogenic Escherichia coli

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