Characterization of maize endosperm-derived suspension cells throughout the culture cycle and enhancement of tissue friability

Abstract

Batch suspension cultures derived from developing maize (Zea mays L.) endosperm were examined throughout the culture cycle to determine the interaction between the tissue and the medium in relation to sugar transport and the effect of subculturing procedures on growth and friability. The growth rate and friability were improved by increasing the frequency of subculture or by physical screening during transfer. An increase in the conductivity of the medium preceded a decrease in fresh weight associated with tissue senescence. Sucrose in the medium was rapidly hydrolysed, and fructose was depleted more rapidly than glucose. Tissue sucrose concentration expressed on a dry weight basis was higher during the middle of the growth cycle, but hexose, starch, zein, lipid, and soluble protein levels changed very little. The medium pH declined from 5.2 to about 4.5 within one day of subculture. Medium pH changed to 4.5 within one day regardless of initial pH (3.0 to 7.0), indicating regulation of external pH rather than passive acidification. Results are consistent with studies of sugar uptake by these cultures, and indicate that cell clump size can be manipulated without exogenous auxin. The characterization of this tissue line establishes its suitability as a model system for studies of sugar transport and other biochemical events in developing maize endosperm.

Degradation and utilization by Butyrivibrio fibrisolvens H17c of xylans with different chemical and physical properties

“…Cellular carbohydrate contents of cultures grown to similar extents on completely utilized soluble sugars indicate that the contribution of microbial carbohydrate to the residual carbohydrate is small (less than 30 g of xylose equivalents per ml as measured with the orcinol reaction). (15), which were then lyophilized to dryness. The dried residue was suspended in a small amount of water, dialyzed against distilled water to remove salts and sugars, and then lyophilized to dryness to yield the CX residue.…”

Metabolism and Synthesis of Indole-3-Acetic Acid (IAA) in Zea mays (Levels of IAA during Kernel Development and the Use of in Vitro Endosperm Systems for Studying IAA Biosynthesis)

“…This cell line was established in 1986 from kemels 10 DAP. A complete description of the initiation and characterization of maize endosperm suspension cultures has been previously reported (Shannon, 1982;Felker et al, 1989). The cells were grown on liquid media, pH 5.6, containing Murashige-Skoog salts (Murashige and Skoog, 1962), 3% SUC, 2 g/L Asn, and 0.4 mg/L thiamine HC1 (Shannon, 1982).…”

Analysis of soybean tissue culture protein dynamics using difference gel electrophoresis

“…Typically, by the end of a culture cycle the cells are starved for reduced carbon and nitrogen [57], and these nutrients are rapidly taken up after the cells are transferred to fresh medium [58]. After uptake, sucrose must be converted to fructose plus UDPgluctose by sucrose synthase (SuSy) [59] before further metabolism.…”