Characterization of Magnetic Markers and Sensors for Liquid-Phase Immunoassays Using Brownian Relaxation

Bhuiya, Anwarul Kabir; Asai, Masayoshi; Watanabe, H.; Hirata, Toyoaki; Higuchi, Yoshinori; Yonetani, Takashi; Enpuku, Keiji

doi:10.1109/tmag.2012.2194137

Cited by 12 publications

(6 citation statements)

References 13 publications

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“…A highly interesting aspect rests in the low detection limit for biotin, achieving a concentration of 9.5 × 10 −16 mol/L. An original comparison of three different magnetic methods was presented by a team of Japanese researchers; 471 the paper focused on SQUID, an MR sensor, and a fluxgate sensor. In addition to this problem, the authors discussed using magnetic markers with a large magnetic moment to attain a higher signal.…”

Section: Chemical Reviewsmentioning

confidence: 99%

Nanoparticle-Based Immunochemical Biosensors and Assays: Recent Advances and Challenges

et al. 2017

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We review the progress achieved during the recent five years in immunochemical biosensors (immunosensors) combined with nanoparticles for enhanced sensitivity. The initial part introduces antibodies as classic recognition elements. The optical sensing part describes fluorescent, luminescent, and surface plasmon resonance systems. Amperometry, voltammetry, and impedance spectroscopy represent electrochemical transducer methods; electrochemiluminescence with photoelectric conversion constitutes a widely utilized combined method. The transducing options function together with suitable nanoparticles: metallic and metal oxides, including magnetic ones, carbon-based nanotubes, graphene variants, luminescent carbon dots, nanocrystals as quantum dots, and photon up-converting particles. These sources merged together provide extreme variability of existing nanoimmunosensing options. Finally, applications in clinical analysis (markers, tumor cells, and pharmaceuticals) and in the detection of pathogenic microorganisms, toxic agents, and pesticides in the environmental field and food products are summarized.

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Section: Chemical Reviewsmentioning

confidence: 99%

Nanoparticle-Based Immunochemical Biosensors and Assays: Recent Advances and Challenges

et al. 2017

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“…Moreover, the effectivity of iron quantification by SQUID magnetic relaxometry was successfully used to evaluate SPIONs conjugated to Her2-expressingMCF/Her2-18 cells (breast cancer cells) injected into xenograft MCF7/Her2-18 tumors in nude mice [82]. Brown relaxation of magnetic NPs in liquids has also been exploited to detect biomarkers in liquid-phase immunoassays using SQUID magnetometry of magnetite beads conjugated with biotin, with sizes from 54 to 322 nm, attaining a sensitivity of 5.6 × 10 −8 mol/mL [83].…”

Section: Superconducting Quantum Interference Devicementioning

confidence: 99%

Hybrid Nanostructured Magnetite Nanoparticles: From Bio-Detection and Theragnostics to Regenerative Medicine

et al. 2020

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Nanotechnology offers the possibility of operating on the same scale length at which biological processes occur, allowing to interfere, manipulate or study cellular events in disease or healthy conditions. The development of hybrid nanostructured materials with a high degree of chemical control and complex engineered surface including biological targeting moieties, allows to specifically bind to a single type of molecule for specific detection, signaling or inactivation processes. Magnetite nanostructures with designed composition and properties are the ones that gather most of the designs as theragnostic agents for their versatility, biocompatibility, facile production and good magnetic performance for remote in vitro and in vivo for biomedical applications. Their superparamagnetic behavior below a critical size of 30 nm has allowed the development of magnetic resonance imaging contrast agents or magnetic hyperthermia nanoprobes approved for clinical uses, establishing an inflection point in the field of magnetite based theragnostic agents.

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“…This type of magnetorelaxometry platform also allows kinetic studies of antibody-antigen reactions in whole blood samples [ 221 ]. Relevant liquid-phase immunoassays use the Brownian relaxation of labeled MPs to detect biotin and distinguish between bound and unbound MPs without requiring a washing step [ 193 ].…”

Section: Detection Techniques and Their Applicationsmentioning

confidence: 99%

Biosensing Using Magnetic Particle Detection Techniques

Chen

Kolhatkar

Zenasni

et al. 2017

Sensors

144

100

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Magnetic particles are widely used as signal labels in a variety of biological sensing applications, such as molecular detection and related strategies that rely on ligand-receptor binding. In this review, we explore the fundamental concepts involved in designing magnetic particles for biosensing applications and the techniques used to detect them. First, we briefly describe the magnetic properties that are important for bio-sensing applications and highlight the associated key parameters (such as the starting materials, size, functionalization methods, and bio-conjugation strategies). Subsequently, we focus on magnetic sensing applications that utilize several types of magnetic detection techniques: spintronic sensors, nuclear magnetic resonance (NMR) sensors, superconducting quantum interference devices (SQUIDs), sensors based on the atomic magnetometer (AM), and others. From the studies reported, we note that the size of the MPs is one of the most important factors in choosing a sensing technique.

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Characterization of Magnetic Markers and Sensors for Liquid-Phase Immunoassays Using Brownian Relaxation

Cited by 12 publications

References 13 publications

Nanoparticle-Based Immunochemical Biosensors and Assays: Recent Advances and Challenges

Nanoparticle-Based Immunochemical Biosensors and Assays: Recent Advances and Challenges

Hybrid Nanostructured Magnetite Nanoparticles: From Bio-Detection and Theragnostics to Regenerative Medicine

Biosensing Using Magnetic Particle Detection Techniques

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