Characterization of M1 and M2 polarization phenotypes in peritoneal macrophages after treatment with graphene oxide nanosheets

Feito, María José; Diez-Orejas, Rosalı́a; Cicuéndez, Mónica; Casarrubios, Laura; Rojo, José María; Portolés, María Teresa

doi:10.1016/j.colsurfb.2018.12.063

Cited by 55 publications

(44 citation statements)

References 52 publications

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“…Other studies have displayed a decrease in CD80 expression after 48 h incubation with GO nanosheets in murine peritoneal M1 macrophages and an increase in CD206 expression in murine M2 macrophages. [ 26 ] For MoS 2 , there has been no published data on activation markers although soluble Mo alone was found to upregulate CD80 and CD86 expression in human macrophages. [ 27 ]…”

Section: Resultsmentioning

confidence: 99%

Comparative Effects of Graphene and Molybdenum Disulfide on Human Macrophage Toxicity

Lin

Lucherelli

et al. 2020

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Graphene and other 2D materials, such as molybdenum disulfide, have been increasingly used in electronics, composites, and biomedicine. In particular, MoS2 and graphene hybrids have attracted a great interest for applications in the biomedical research, therefore stimulating a pertinent investigation on their safety in immune cells like macrophages, which commonly engulf these materials. In this study, M1 and M2 macrophage viability and activation are mainly found to be unaffected by few‐layer graphene (FLG) and MoS2 at doses up to 50 µg mL−1. The uptake of both materials is confirmed by transmission electron microscopy, inductively coupled plasma mass spectrometry, and inductively coupled plasma atomic emission spectroscopy. Notably, both 2D materials increase the secretion of inflammatory cytokines in M1 macrophages. At the highest dose, FLG decreases CD206 expression while MoS2 decreases CD80 expression. CathB and CathL gene expressions are dose‐dependently increased by both materials. Despite a minimal impact on the autophagic pathway, FLG is found to increase the expression of Atg5 and autophagic flux, as observed by Western blotting of LC3‐II, in M1 macrophages. Overall, FLG and MoS2 are of little toxicity in human macrophages even though they are found to trigger cell stress and inflammatory responses.

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Section: Resultsmentioning

confidence: 99%

Comparative Effects of Graphene and Molybdenum Disulfide on Human Macrophage Toxicity

Lin

Lucherelli

et al. 2020

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“…Indeed, the selection of the most accurate molecular markers to determine macrophage M1 and M2 polarization is still a challenging aspect that needs further research [2]. Previous immunohistochemical studies have broadly used CD68 marker to identify macrophages [3,7,15], CD80 marker to identify M1 phenotype [23][24][25] and CD206 to detect M2 phenotype [2, 7,25]. However, these markers can stain other cells such as fibroblasts, dendritic cells, sub-population of endothelial cells, and B cells [25][26][27].…”

Section: Discussionmentioning

confidence: 99%

Macrophage polarization in peri-implantitis lesions

et al. 2020

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Objectives To immunohistochemically characterize and correlate macrophage M1/M2 polarization status with disease severity at peri-implantitis sites. Materials and methods A total of twenty patients (n = 20 implants) diagnosed with peri-implantitis (i.e., bleeding on probing with or without suppuration, probing depths ≥ 6 mm, and radiographic marginal bone loss ≥ 3 mm) were included. The severity of peri-implantitis was classified according to established criteria (i.e., slight, moderate, and advanced). Granulation tissue biopsies were obtained during surgical therapy and prepared for immunohistological assessment and macrophage polarization characterization. Macrophages, M1, and M2 phenotypes were identified through immunohistochemical markers (i.e., CD68, CD80, and CD206) and quantified through histomorphometrical analyses. Results Macrophages exhibiting a positive CD68 expression occupied a mean proportion of 14.36% (95% CI 11.4-17.2) of the inflammatory connective tissue (ICT) area. Positive M1 (CD80) and M2 (CD206) macrophages occupied a mean value of 7.07% (95% CI 5.9-9.4) and 5.22% (95% CI 3.8-6.6) of the ICT, respectively. The mean M1/M2 ratio was 1.56 (95% CI 1-12-1.9). Advanced peri-implantitis cases expressed a significantly higher M1 (%) when compared with M2 (%) expression. There was a significant correlation between CD68 (%) and M1 (%) expression and probing depth (PD) values. Conclusion The present immunohistochemical analysis suggests that macrophages constitute a considerable proportion of the inflammatory cellular composition at peri-implantitis sites, revealing a significant higher expression for M1 inflammatory phenotype at advanced peri-implantitis sites, which could possibly play a critical role in disease progression. Clinical relevance Macrophages have critical functions to establish homeostasis and disease. Bacteria might induce oral dysbiosis unbalancing the host's immunological response and triggering inflammation around dental implants. M1/M2 status could possibly reveal peri-implantitis' underlying pathogenesis.

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“…AuNPs were one of the commonly used inorganic nanocarriers. Under the irradiation of near infrared light, they would generate heat and reactive oxygen to promote cell apoptosis.15nm-sized gold nanoparticles were experimentally proven to be less toxic than other-sized nanoparticles, which owed better tissue penetration and would not be recognized and cleared in the blood circulation [23]. PDA had low toxicity and was easily soluble in water, which could improve the toxicity and biocompatibility of the gold nanocomposite.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, PDA shell can prevent the leakage of loading drugs during delivery, while achieve an on-demand drug release in the targeted location, such as NIR stimuli responsive drug release under high temperature or acidic conditions [19][20][21]. PEG can also improve the ability of cells to take up gold nanocomposite and prolong the cycle times in plasma [22][23][24]. In addition, drugs linked to PEG will be released under acidic conditions, which can lay the foundation for hierarchical drug release system [25].…”

Section: Introductionmentioning

confidence: 99%

Hierarchical Drug Release Designed Au @PDA-PEG-MTX NPs for Targeted Delivery to Breast Cancer with Combined Photothermal-chemotherapy

Cao

et al. 2021

Preprint

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Breast cancer (BC) is the frequently diagnosed cancer and one of the deadliest causes of cancer-related death with a severe survival rate. Methotrexate (MTX) is an anti-tumor drug used in the treatment of BC. The poor dispersion in water and toxic side effects limit its clinical application. Gold nanoparticles (AuNPs), due to their specific structures and unique biological and physiochemical properties, have emerged as attractive candidates as vehicles for tumor targeting, bio-imaging and therapy. An innovative nano drug-loading system (Au @PDA-PEG-MTX NPs) was prepared for targeted treatment of BC. Au @PDA-PEG-MTX NPs under near infra-red region (NIR) irradiation showed effective photothermal therapy against MDA-MB-231 human BC cells growth in vitro by inducing apoptosis through triggering reactive oxygen species (ROS) overproduction and generating excessive heat. In vivo studies revealed that Au @PDA-PEG-MTX NPs under NIR irradiation showed deep penetration and cancer-targeted fluorescence imaging application and strong photothermal therapy against BC xenograft growth in vivo by induction of apoptosis. Analysis of histopathology, cellular uptake, cytotoxicity assay, apoptosis experiment indicated that Au @PDA-PEG-MTX NPs had a good therapeutic effect with high biocompatibility and less side effect. This Au NPs drug-loading system achieved specific BC targeting ability by surface decoration of MTX, NIR laser irradiation for fluorescence imaging and combined photothermal-chemotherapy as well as pH- and NIR- triggered hierarchical drug release.

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Characterization of M1 and M2 polarization phenotypes in peritoneal macrophages after treatment with graphene oxide nanosheets

Abstract: El artículo seleccionado no se encuentra disponible por ahora a texto completo por no haber sido facilitado todavía por el investigador a cargo del archivo del mismo.

Cited by 55 publications

References 52 publications

Comparative Effects of Graphene and Molybdenum Disulfide on Human Macrophage Toxicity

Comparative Effects of Graphene and Molybdenum Disulfide on Human Macrophage Toxicity

Macrophage polarization in peri-implantitis lesions

Hierarchical Drug Release Designed Au @PDA-PEG-MTX NPs for Targeted Delivery to Breast Cancer with Combined Photothermal-chemotherapy

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