Characterization of lymph node optical properties for phantom fabrication

Torres, Veronica C.; Wilson, Todd; Li, Chengyue; Sinha, Lagnojita; Brankov, Jovan G.; Tichauer, Kenneth M.

doi:10.1117/12.2513245

Cited by 2 publications

(2 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Jurkat cells within the EVA semi-transparent bag are characterized by a higher reduced scattering coefficient (around 3 cm −1 ) and a low absorption (0.02 cm −1 ) as estimated through calculations. 37 Both of the semi-transparent top layers (PVC and EVA) have very similar optical properties with an extinction coefficient at 830 nm of practically zero. 38,39…”

Section: Resultsmentioning

confidence: 99%

Defocused Spatially Offset Raman Spectroscopy in Media of Different Optical Properties for Biomedical Applications Using a Commercial Spatially Offset Raman Spectroscopy Device

et al. 2019

View full text Add to dashboard Cite

In this study, we show how defocused spatially offset Raman spectroscopy (SORS) can be employed to recover chemical information from media of biomedical significance within sealed plastic transfusion and culture bags using a commercial SORS instrument. We demonstrate a simple approach to recover subsurface spectral information through a transparent barrier by optimizing the spatial offset of the defocused beam. The efficiency of the measurements is assessed in terms of the SORS ratio and signal-to-noise ratio (S/N) through a simple manual approach and an ordinary least squares model. By comparing the results for three different biological samples (red blood cell concentrate, pooled red cell supernatant and a suspension of Jurkat cells), we show that there is an optimum value of the offset parameter which yields the maximum S/N depending on the barrier material and optical properties of the ensemble contents. The approach was developed in the context of biomedical applications but is generally applicable to any three-layer system consisting of turbid content between transparent thin plastic barriers (i.e., front and back bag surfaces), particularly where the analyte of interest is dilute or not a strong scatterer.

show abstract

Section: Resultsmentioning

confidence: 99%

Defocused Spatially Offset Raman Spectroscopy in Media of Different Optical Properties for Biomedical Applications Using a Commercial Spatially Offset Raman Spectroscopy Device

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Two batches of each phantom were made, for a total of 22 test samples. The mixtures were cast in square molds to form 2x2x1 cm 3 cuboids, and underwent two rounds of degassing to remove air bubbles before being left to cure for 24 h. After demolding, the absorption coefficient, 𝜇 a , and reduced scattering coefficient, µ s ', of each sample was estimated using a time-resolved method [2]. Based on the results, a composition of 0.5% v/v of stock TiO 2 was selected to match the optical properties of lymph nodes.…”

mentioning

confidence: 99%

Supplementary document for Characterization of an angular domain fluorescence optical projection tomography system for mesoscopic lymph node imaging - 4867069.pdf

Torres,

Li,

Zhou

et al. 2020

View full text Add to dashboard Cite

Characterization of lymph node optical properties for phantom fabrication

Cited by 2 publications

References 19 publications

Defocused Spatially Offset Raman Spectroscopy in Media of Different Optical Properties for Biomedical Applications Using a Commercial Spatially Offset Raman Spectroscopy Device

Defocused Spatially Offset Raman Spectroscopy in Media of Different Optical Properties for Biomedical Applications Using a Commercial Spatially Offset Raman Spectroscopy Device

Supplementary document for Characterization of an angular domain fluorescence optical projection tomography system for mesoscopic lymph node imaging - 4867069.pdf

Contact Info

Product

Resources

About