Characterization of LpGPAT Gene in<i>Lilium pensylvanicum</i>and Response to Cold Stress

Sun, Shaokun; Yang, Ning; Chen, Li-Jing; Irfan, Muhammad; Zhao, Xinghua; Li, Tianlai

doi:10.1155/2015/792819

Cited by 7 publications

(6 citation statements)

References 12 publications

(13 reference statements)

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“…The GPAT gene family has been identified in many model plants, including Arabidopsis thaliana [ 8 , 10 ], Brassica napus [ 11 ], Oryza sativa [ 10 ], Zea mays [ 12 ], and other plants, such as Paeonia [ 13 ], Gossypium [ 14 ], and Perilla frutescens [ 15 ]. It has been confirmed that the members of the GPAT gene family have been widely described for their role in male fertility [ 16 , 17 , 18 , 19 , 20 , 21 ], seed development [ 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ], biotic and abiotic stress tolerance [ 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 ], and cuticle or suberin production [ 5 , 22 , 44 , 45 , 46 , 47 , 48 , 49 ]. At present, the research on the GPAT gene family is the best in the model plant Arabidopsis.…”

Section: Introductionmentioning

confidence: 99%

“…The function of GPAT genes under abiotic stresses such as drought, salinity, low temperature, and high temperature has been studied in many plants [ 33 , 34 , 36 , 37 , 38 , 40 , 41 , 43 , 53 ]. It is reported that the overexpression of the AtS1 gene improves the tolerance of Arabidopsis seedlings to low temperature stress, while the transformation of the squash GPAT gene into tobacco leads to a reduction in low temperature tolerance [ 31 , 40 ].…”

Section: Introductionmentioning

confidence: 99%

“…Overexpression of the AmGPAT gene in Ammopiptanthus mongolicus can improve the tolerance of transgenic Arabidopsis to cold and other oxidative stress by increasing the level of unsaturated fatty acids in phosphatidylglycerol [ 40 ]. In another study, the LpGPAT gene in Lilium pensylvanicum was proved to be related to low-temperature stress [ 36 ]. Overexpression of the SsGPAT gene in Suaeda salsa enhanced salt tolerance in Arabidopsis [ 37 ],while loss-of-function of ATS1 enhances Arabidopsis salt tolerance [ 43 ].…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Identification, Characterization, Evolutionary Analysis, and Expression Pattern of the GPAT Gene Family in Barley and Functional Analysis of HvGPAT18 under Abiotic Stress

Yang,

Ma,

et al. 2024

IJMS

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Glycerol-3-phosphoacyltransferase (GPAT) is an important rate-limiting enzyme in the biosynthesis of triacylglycerol (TAG), which is of great significance for plant growth, development, and response to abiotic stress. Although the characteristics of GPAT have been studied in many model plants, little is known about its expression profile and function in barley, especially under abiotic stress. In this study, 22 GPAT genes were identified in the barley genome and divided into three groups (I, II, III), with the latter Group III subdivided further into three subgroups based on the phylogenetic analysis. The analyses of conserved motifs, gene structures, and the three-dimensional structure of HvGPAT proteins also support this classification. Through evolutionary analysis, we determined that HvGPATs in Group I were the earliest to diverge during 268.65 MYA, and the differentiation of other HvGPATs emerged during 86.83–169.84 MYA. The tissue expression profile showed that 22 HvGPAT genes were almost not expressed in INF1 (inflorescence 1). Many functional elements related to stress responses and hormones in cis-element analysis, as well as qRT-PCR results, confirm that these HvGPAT genes were involved in abiotic stress responses. The expression level of HvGPAT18 was significantly increased under abiotic stress and its subcellular localization indicated its function in the endoplasmic reticulum. Various physiological traits under abiotic stress were evaluated using transgenic Arabidopsis to gain further insight into the role of HvGPAT18, and it was found that transgenic seedlings have stronger resistance under abiotic stress than to the wild-type (WT) plants. Overall, our results provide new insights into the evolution and function of the barley GPAT gene family and enable us to explore the molecular mechanism of functional diversity behind the evolutionary history of these genes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Identification, Characterization, Evolutionary Analysis, and Expression Pattern of the GPAT Gene Family in Barley and Functional Analysis of HvGPAT18 under Abiotic Stress

Yang,

Ma,

et al. 2024

IJMS

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“…In Arabidopsis, ten GPAT homologs have been identified, of which GPAT1~8 are the land-plant-specific sn-2-GPAT that is mainly involved in the biosynthesis of lipid polyesters such as cutin and suberin, and is associated with plant flower development and stress response (Li et al, 2007;Li-Beisson et al, 2009;Chen et al, 2011;Yang et al, 2012). Plastid-localized ATS1 also can catalyze the acylation reaction at the sn-1 site of G3P using acyl-ACP as an acyl donor, and may be related to the mechanism of plant cold tolerance response (Paya-Milans et al, 2015;Sun et al, 2015). Loss of function of GPAT1 located in mitochondria alters the fatty acid composition in floral tissues and seeds, but its effect on seed oil content is still controversial (Zheng et al, 2003;Bai et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Characterization and functional analysis of AhGPAT9 gene involved in lipid synthesis in peanut (Arachis hypogaea L.)

Shen

Shen²,

Liu³

et al. 2023

Front. Plant Sci.

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GPAT enzymes (glycerol-3-phosphate 1-O-acyltransferase, EC 2.3.1.15) catalyze the initial and rate-limiting step of plant glycerolipid biosynthesis for membrane homeostasis and lipid accumulation, yet little research has been done on peanuts. By reverse genetics and bioinformatics analyses, we have characterized an AhGPAT9 isozyme, of which the homologous product is isolated from cultivated peanut. QRT-PCR assay revealed a spatio-temporal expression pattern that the transcripts of AhGPAT9 accumulating in various peanut tissues are highly expressed during seed development, followed by leaves. Green fluorescent protein tagging of AhGPAT9 confirmed its subcellular accumulation in the endoplasmic reticulum. Compared with the wild type control, overexpressed AhGPAT9 delayed the bolting stage of transgenic Arabidopsis, reduced the number of siliques, and increased the seed weight as well as seed area, suggesting the possibility of participating in plant growth and development. Meanwhile, the mean seed oil content from five overexpression lines increased by about 18.73%. The two lines with the largest increases in seed oil content showed a decrease in palmitic acid (C16:0) and eicosenic acid (C20:1) by 17.35% and 8.33%, respectively, and an increase in linolenic acid (C18:3) and eicosatrienoic acid (C20:3) by 14.91% and 15.94%, respectively. In addition, overexpressed AhGPAT9 had no significant effect on leaf lipid content of transgenic plants. Taken together, these results suggest that AhGPAT9 is critical for the biosynthesis of storage lipids, which contributes to the goal of modifying peanut seeds for improved oil content and fatty acid composition.

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“…qRT-PCR is commonly used to analyze gene expression in Lilium . To date, the traditional housekeeping genes UBQ [ 31 ], GAPDH [ 32 , 33 ], 18S [ 21 ], and ACT [ 2 , 34 , 35 ] have been used as internal reference genes to standardize the expression profile of some genes. However, the relative stability of these and other potential reference genes in Lilium has not been validated in a range of experimental contexts, and this has constrained the wider use of qRT-PCR in Lilium .…”

Section: Introductionmentioning

confidence: 99%

Validation of Reference Genes for Accurate Normalization of Gene Expression in Lilium davidii var. unicolor for Real Time Quantitative PCR

Cheng

Zhang

et al. 2015

PLoS ONE

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Lilium is an important commercial market flower bulb. qRT-PCR is an extremely important technique to track gene expression levels. The requirement of suitable reference genes for normalization has become increasingly significant and exigent. The expression of internal control genes in living organisms varies considerably under different experimental conditions. For economically important Lilium, only a limited number of reference genes applied in qRT-PCR have been reported to date. In this study, the expression stability of 12 candidate genes including α-TUB, β-TUB, ACT, eIF, GAPDH, UBQ, UBC, 18S, 60S, AP4, FP, and RH2, in a diverse set of 29 samples representing different developmental processes, three stress treatments (cold, heat, and salt) and different organs, has been evaluated. For different organs, the combination of ACT, GAPDH, and UBQ is appropriate whereas ACT together with AP4, or ACT along with GAPDH is suitable for normalization of leaves and scales at different developmental stages, respectively. In leaves, scales and roots under stress treatments, FP, ACT and AP4, respectively showed the most stable expression. This study provides a guide for the selection of a reference gene under different experimental conditions, and will benefit future research on more accurate gene expression studies in a wide variety of Lilium genotypes.

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Characterization of LpGPAT Gene inLilium pensylvanicumand Response to Cold Stress

Cited by 7 publications

References 12 publications

Genome-Wide Identification, Characterization, Evolutionary Analysis, and Expression Pattern of the GPAT Gene Family in Barley and Functional Analysis of HvGPAT18 under Abiotic Stress

Genome-Wide Identification, Characterization, Evolutionary Analysis, and Expression Pattern of the GPAT Gene Family in Barley and Functional Analysis of HvGPAT18 under Abiotic Stress

Characterization and functional analysis of AhGPAT9 gene involved in lipid synthesis in peanut (Arachis hypogaea L.)

Validation of Reference Genes for Accurate Normalization of Gene Expression in Lilium davidii var. unicolor for Real Time Quantitative PCR

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