Characterization of lipids A of Ralstonia solanacearum lipopolysaccharides

Abstract

The analysis of fatty acid profiles of lipopolysacharid.es has shown that R. solanacearum strains tested may be divided into two groups. The first group is represented by R. solanacearum strains (5712, 7945, 7955 and 8110) the lipids A of which contained hydroxylated fatty acids with long chains: 3-hydroxy tetradecanoic, 2-hydroxyhexadecanoic and 2-hydroxyoctadecanoic. The second group was represented by R. solanacearum strains the lipids A of which contained hydroxylated fatty acids with short chains: 3-hydroxydecanoic, 2-hydroxydodecanoic and 3-hydroxydodecanoic. 3-hydroxytetradecanoic acid was observed in a small amount. A comparative analysis of the fatty acid composition and biological activity gives a possibility to suppose that 3-hydroxytetradecanoic, 2-hydroxyhexadecanoic and 2-hydroxy octadecanoic acids may be responsible for the toxicity and pyrogenicity of the lipopolysaccharides tested.

Fatty Acid Polymorphism of Ralstonia solanacearum in Different Egyptian Governorates and other European Countries

“…In the present study, fatty acid composition in all isolates differ in presence of short or long chains of hydroxylated fatty acids therefore, it clustered in two groups; group I contains European isolates with long chains-hydroxylated fatty acids while, group II contains Egyptian isolates with short chains of fatty acids ( Fig. 1) (Varbanets et al, 2004).…”

“…The first group was represented by R. solanacearum isolates from European countries RsFr4, RsSc1 and RsNe1 which contained the hydroxylated fatty acids with long chains including: 3-hydroxytetradecanoic with C14 length chain, 2-hydroxyhexadecanoic (C16) and 2-hydroxyoctadecanoic from C18 to C26 while, the second group went to the Egyptian isolates (Table 3) which contained the hydroxylated fatty acids with short chains vary from C10; 2-hydroxydodecanoic ,C11 and C12; 3-hydroxydodecanoic (Varbanets et al, 2004).…”

“…Previous reports have indicated that fatty acid methyl ester profiling corresponded well, to be the best phenotypic marker for R. solanacearum isolates classification (Weller et al, 2000;Varbanets et al, 2003Varbanets et al, , 2004Dawyndt et al, 2006;Tohamy et al, 2007;Khakvar et al, 2011). The objective of this study was to characterize the potato bacterial wilt R. solanacearum isolates collected from Egypt and European countries based on their pathogenicity and the polymorphism of fatty acid profiles.…”

Biochemical Characterization of Lipopolysaccharide of Ralstonia solanacearum and its Relation to Virulence

“…Cells were centrifuged at 8000g at 4°C for 30 min. The wet cells were suspended with acetone and diethyl ether (Varbanets et al, 2004), and weighed sample of cell was extracted three times at 68°C with a 45% solution of phenol. After each extraction, the sample was cooled to 10 °C and centrifuged at 1,000 g for 45 min.…”

“…show reduced virulence and the number of viable bacteria in plant tissues declines rapidly (Esposito et al, 2008). LPS are ubiquitous, indispensable components of the cell surface of gram-negative bacteria and their taxonomy that apparently have diverse roles in bacterial pathogensis of plant (Erbs andNewman, 2003 andVarbanets et al, 2004). Abundant Exopolysaccharide (EPS) produced by pathogenic strains of Ralstonia solanacearum on solid medium and in planta has long been correlated with its pathogenicity (Araud-Razou et al, 1998 andEsposito et al, 2008).…”