Characterization of LH/hCG Receptor Binding Inhibitor in Corpora Lutea of Human and Sheep Ovaries

Kumari, G.L.; Kumar, Narender; Duraiswami, S.; Datta, Jhuma; Vidyasagar, I. C.; Vohra, S.; Roy, Somnath

doi:10.1007/978-1-4615-9278-5_16

Cited by 3 publications

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“…Channing et al (8), Kumari et al. (9) and Ward et al (10) have purified partially those fractions containing LH-RBI activity from porcine corpora lutea. Administration of LH-RBI preparation either immediately prior to mating or on day 1 or 2 following mating inhibited pregnancy in the rabbit (8).…”

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confidence: 99%

Identification of luteinizing hormone receptor binding inhibitor in bovine corpora lutea

Rathnam

Lin²,

Saxena³

1995

European Journal of Endocrinology

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A 7000 g supernatant, obtained during the purification of luteinizing hormone (LH) receptor from bovine corpora lutea homogenate, was concentrated by ultrafiltration. The filtrate, containing < 50,000 molecular weight material, exhibited LH receptor binding inhibitor (LH-RBI) activity. The filtrate was ultrafiltered sequentially through Amicon PM-10, PM-30 and UM-2 filters to yield a LH-RBI-containing fraction in the higher molecular weight range of 30,000-10,000 and a LH-RBI-containing fraction in the lower molecular weight range of 10,000-1000. The higher molecular weight LH-RBI fraction was purified on Sephadex G-25 and the lower molecular weight LH-RBI fraction was purified on Sephadex G-50. Both the high- and the low-molecular-weight LH-RBI species inhibited the binding of 125I-labeled human chorionic gonadotropin (hCG) to bovine corpora lutea and to rat Leydig cell membrane receptors. Similarly, the production of testosterone by hCG-stimulated rat Leydig cells was inhibited in a dose-response manner by both the high- and the low-molecular-weight LH-RBI species. The LH-RBI activity in the low-molecular-weight species was stable at 4 degrees C for up to 6 months and at temperatures up to 90 degrees C for 15 mins, whereas the LH-RBI activity of the high-molecular-weight species was stable at 4 degrees C for 15 months and unstable at 60 degrees C after 15 min. The 7000 g supernatant provided a much-needed source to obtain larger than previously reported quantities of LH-RBI for isolation as well as for structure and function studies.

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confidence: 99%

Identification of luteinizing hormone receptor binding inhibitor in bovine corpora lutea

Rathnam

Lin²,

Saxena³

1995

European Journal of Endocrinology

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“…Lorsqu'il est ajouté au milieu de culture de cellules de granulosa, le liquide folliculaire, en fonction des follicules desquels il est ré cupé ré , ré gule positivement ou né gativement la sé cré tion de progesté rone et la luté inisation des cellules [14]. Par ailleurs, il contient des facteurs capables d'inhiber l'activité de la FSH au niveau des cellules folliculaires.…”

Section: Prolife´ration Et Diffe´renciation Des Cellules Somatiquesunclassified

Le liquide folliculaire chez les mammifères

Fahiminiya

Gérard

2010

Gynécologie Obstétrique & Fertilité

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Isolation of Luteinizing Hormone Receptor Binding Inhibitor from Ovine Corpora Lutea

et al. 1988

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Procedures for the isolation of luteinizing hormone receptor binding inhibitor (LHRBI) from crude extracts of ovine corpora lutea are described. Microsomal pellets recovered by differential centrifugation of homogenates of fresh corpora lutea were stored at -20 degrees C for 3 weeks and then extracted with Tris-HCl buffer (pH 7.4). On sequential filtration of the extract on Amicon UM-20, PM-10 and UM-2R filters, the corresponding retentates (UM-20R, PM-10R and UM-2R) demonstrated inhibition of radio-labeled human chorionic gonadotrophin (hCG) to ovine luteal cells. The retentate of UM-2 filter (UM-2R) was further fractionated by reverse phase high pressure liquid chromatography. The active fractions- III, IX, X and XIII, thus obtained, calculated by their ability to inhibit 125I-hCG binding in dose dependent manner, were purified 150 to 1000-fold.

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Characterization of LH/hCG Receptor Binding Inhibitor in Corpora Lutea of Human and Sheep Ovaries

Cited by 3 publications

References 5 publications

Identification of luteinizing hormone receptor binding inhibitor in bovine corpora lutea

Identification of luteinizing hormone receptor binding inhibitor in bovine corpora lutea

Le liquide folliculaire chez les mammifères

Isolation of Luteinizing Hormone Receptor Binding Inhibitor from Ovine Corpora Lutea

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