Characterization of leukotriene B4 synthesis in canine polymorphonuclear leukocytes

Amalsadvala, Tannaz; Vaughn, Dana M.

doi:10.1016/0952-3278(92)90084-v

Cited by 6 publications

(1 citation statement)

References 24 publications

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“…Thrombocytes and white blod cells (WBC) were isolated from fresh blood within 5 min of collection using a modified version of the methods described by Amalsadvala and Vaughn (1992). Briefly, a stock solution of histopaque 1119 was prepared by adding 1.5 g of sodium diatriazoate to 100 mL of histopaque 1119.…”

Section: Isolation Of Thrombocytes and White Blood Cellsmentioning

confidence: 99%

Dietary n-3 fatty acids decrease the leukotriene B₄ response ex vivo and the bovine serum albumin-induced footpad swelling index in New Hampshire hens

Hall¹,

Jha²,

Cherian³

2007

Can. J. Anim. Sci.

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The effects of feeding New Hampshire hens different amounts of n-6 and n-3 fatty acids on plasma and immune tissue fatty acid profiles, leukotriene (LT) B4 response ex vivo in stimulated thrombocytes, serum non-esterified fatty acid (NEFA) concentrations, and bovine serum albumen (BSA)-induced footpad swelling index were investigated. Seventy-two New Hampshire hens (n = 24/treatment) were fed diets supplemented with 3.0% sunflower oil (Diet I), 1.5% sunflower oil + 1.5% fish oil (Diet II), or 3.0% fish oil (Diet III). After 46 d on experimental diets, concentrations of C20:5n-3 and C22:6n-3 were higher in plasma, thrombocytes, and spleens of hens fed Diet II and III compared with hens fed Diet I (P ≤ 0.05). Thrombocytes from hens fed Diet III that were stimulated ex vivo with calcium ionophore A23187 produced less LTB4 than those from hens fed Diets I and II (P < 0.05). The serum NEFA concentrations were 0.31 ± 0.12, 0.25 ± 0.08, and 0.17 ± 0.06 mEq L-1 for hens fed Diets I, II, and III, respectively (P = 0.07). The cell-mediated immune response, based on results of a BSA-induced footpad swelling index at 48 h, was less in hens fed Diet III (P < 0.05) compared with hens fed Diets I or II. These results indicate that increasing the concentration of n-3 fatty acids in diets fed to New Hampshire hens might depress cell-mediated immunity and the pro-inflammatory LTB4 response. Key words: N-3 fatty acids; Leukotriene B4; Thrombocytes

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Section: Isolation Of Thrombocytes and White Blood Cellsmentioning

confidence: 99%

Dietary n-3 fatty acids decrease the leukotriene B₄ response ex vivo and the bovine serum albumin-induced footpad swelling index in New Hampshire hens

Hall¹,

Jha²,

Cherian³

2007

Can. J. Anim. Sci.

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Optimization of assay conditions for leukotriene B4 synthesis by neutrophils or platelets isolated from peripheral blood of monogastric animals

Jha

Hall

Cherian

et al. 2005

Prostaglandins, Leukotrienes and Essential Fatty Acids

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Maternal dietary n-3 fatty acids alter immune cell fatty acid composition and leukotriene production in growing chicks

Hall

Jha

Skinner

et al. 2007

Prostaglandins, Leukotrienes and Essential Fatty Acids

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Characterization of leukotriene B4 synthesis in canine polymorphonuclear leukocytes

Cited by 6 publications

References 24 publications

Dietary n-3 fatty acids decrease the leukotriene B₄ response ex vivo and the bovine serum albumin-induced footpad swelling index in New Hampshire hens

Dietary n-3 fatty acids decrease the leukotriene B₄ response ex vivo and the bovine serum albumin-induced footpad swelling index in New Hampshire hens

Optimization of assay conditions for leukotriene B4 synthesis by neutrophils or platelets isolated from peripheral blood of monogastric animals

Maternal dietary n-3 fatty acids alter immune cell fatty acid composition and leukotriene production in growing chicks

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Characterization of leukotriene B4 synthesis in canine polymorphonuclear leukocytes

Cited by 6 publications

References 24 publications

Dietary n-3 fatty acids decrease the leukotriene B4 response ex vivo and the bovine serum albumin-induced footpad swelling index in New Hampshire hens

Dietary n-3 fatty acids decrease the leukotriene B4 response ex vivo and the bovine serum albumin-induced footpad swelling index in New Hampshire hens

Optimization of assay conditions for leukotriene B4 synthesis by neutrophils or platelets isolated from peripheral blood of monogastric animals

Maternal dietary n-3 fatty acids alter immune cell fatty acid composition and leukotriene production in growing chicks

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Dietary n-3 fatty acids decrease the leukotriene B₄ response ex vivo and the bovine serum albumin-induced footpad swelling index in New Hampshire hens

Dietary n-3 fatty acids decrease the leukotriene B₄ response ex vivo and the bovine serum albumin-induced footpad swelling index in New Hampshire hens