Characterization of Leucaena (<em>Leucaena leucephala</em>) oil by direct analysis in real time (DART) ion source and gas chromatography

Abstract

SUMMARY:For the first time, we report the characterization of triacylglycerols and fatty acids in Leucaena (Leucaena leucephala) oil [LUCO], an unexplored nontraditional non-medicinal plant belonging to the family Fabaceae. LUCO was converted to fatty acid methyl esters (FAMEs). We analyzed the triacylglycerols (TAGs) of pure LUCO and their FAMEs by time-of-flight mass spectrometry (TOF-MS) followed by multivariate analysis for discrimination among the FAMEs. Our investigations for the analysis of LUCO samples represent noble features of glycerides. A new type of ion source, coupled with high-resolution TOF-MS was applied for the comprehensive analysis of triacylglycerols. The composition of fatty acid based LUCO oil was studied using Gas Chromatography (GC-FID). The major fatty acid components of LUCO oil are linoleic acid (52.08%) oleic acid (21.26%), palmitic acid (7.91%) and stearic acid (6.01%). A metal analysis in LUCO was done by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The structural elucidation and thermal stability of LUCO were studied by FT-IR, 1 H NMR, 13 C NMR spectroscopic techniques and TGA-DSC, respectively. We also measured the cytotoxicity of LUCO. KEYWORDS: Cytotoxicity; Gas Chromatography; Leucaena oil; Thermal analysis; TOF-MSRESUMEN: Caracterización del aceite de Leucaena (Leucaena leucocephala) por análisis directo en tiempo real (DART) y cromatografía de gases. Se presenta por primera vez la caracterización de triacilgliceroles y ácidos grasos del aceite de Leucaena (Leucaena leucephala) [LUCO], una planta no medicinal, no tradicional y no explorada, perteneciente a la familia Fabaceae. Se analizaron triacilgliceroles (TAGs) de LUCO y sus FAMEs por espectrometría de masas de tiempo de vuelo (TOF-MS) seguido de análisis multivariante para discriminación entre los FAME. Nuestras investigaciones para el análisis de muestras de LUCO presentaron características propias de los glicéridos. Un nuevo tipo de fuente de iones, junto con alta resolución TOF-MS se aplicó para el análisis exhaustivo de triacilgliceroles. La composición de aceite de LUCO basado en ácidos grasos se estudió usando Cromatografía de Gas (GC-FID). Los principales componentes de ácidos grasos del aceite LUCO fueron, linoleico (52,08%), oleico (21,26%), palmítico (7,91%) y esteárico 6,01%. El análisis de metales se realizó mediante Espectrometría de Plasma Acoplado Inductivamente a Masas (ICP-MS). La elucidación estructural y la estabilidad térmica de LUCO se estudiaron mediante FT-IR, 1 H NMR, técnicas espectroscópi-cas de 13 C NMR y TGA-DSC, respectivamente. También se midió la citotoxicidad de LUCO.

Mechanically Strong, Hydrophobic, Antimicrobial, and Corrosion Protective Polyesteramide Nanocomposite Coatings from Leucaena leucocephala Oil: A Sustainable Resource

“…Seeds were separated and powdered, and oil was extracted from powdered seeds through a Soxhlet apparatus using petroleum ether as solvent. The fatty acid composition (linoleic acid 52%, oleic acid 21%, palmitic acid 14%, and stearic acid 6%) of LLO was determined using methyl ester on gas chromatography with an FID detector . Deionized water [DW] was used throughout the experimental steps.…”

“…The fatty acid composition (linoleic acid 52%, oleic acid 21%, palmitic acid 14%, and stearic acid 6%) of LLO was determined using methyl ester on gas chromatography with an FID detector. 5 Deionized water [DW] was used throughout the experimental steps.…”

“…The seed oil of Leucaena leucocephala [LLO] is rich in linoleic, oleic, palmitic, and stearic acid, which comprise more than 92% of total fatty acids of LLO, and also has a higher content of tocopherols . LLO has also shown antibacterial and antifungal properties .…”

“…4 The seed oil of Leucaena leucocephala [LLO] is rich in linoleic, oleic, palmitic, and stearic acid, which comprise more than 92% of total fatty acids of LLO, and also has a higher content of tocopherols. 5 LLO has also shown antibacterial and antifungal properties. 6 The leaves of Leucaena leucocephala plant [LL] cardic glycosides and are useful as antioxidant, antimicrobial, anticancer, and antiparasitic, as pesticide and nematicide, and in other applications.…”