Characterization of Lactobacillus helveticus strains isolated from Bulgarian yoghurt, cheese, plants and human faecal samples by sodium dodecilsulfate polyacrylamide gel electrophoresis of cell-wall proteins, ribotyping and pulsed field gel fingerprinting

Dimitrov, Zhechko; Michaylova, Michaela; Mincova, Svetlana

doi:10.1016/j.idairyj.2004.09.008

Cited by 22 publications

(18 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…helveticus strains and for investigating whether the findings concerning phenotypic discrimination were consistent with the genotypes of the organisms. These methods include analyses of total genomic DNA restriction fragment length polymorphism (RFLP); pulsed-field gel electrophoresis (PFGE); randomly amplified polymorphic DNA (RAPD) or analyses based on ribosomal RNA genes (ribotyping; PCR-ribotyping; rDNA sequence) (Dimitrov, Michaylova, & Mincova, 2005;Giraffa, De Vecchi, Rossi, Nicastro, & Fortina, 1998;Giraffa, Gatti, Rossetti, Senini, & Neviani, 2000;Lombardi et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Multilocus restriction typing: A tool for studying molecular diversity within Lactobacillus helveticus of dairy origin

Borgo

Ricci

Manachini

et al. 2007

International Dairy Journal

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Multilocus restriction typing: A tool for studying molecular diversity within Lactobacillus helveticus of dairy origin

Borgo

Ricci

Manachini

et al. 2007

International Dairy Journal

View full text Add to dashboard Cite

“…SDS-PAGE of whole-cell (Ghazi et al 2009) and cell-wall associated proteins (Dimitrov et al 2005) are phenotypic methods widely used for lactic acid bacteria identification and taxonomic discrimination at species and subspecies level (Ghazi et al 2009). When the standardized conditions of SDS-PAGE are fulfilled, patterns of whole-cell proteins are extremely unfailing and may be correlated with DNA-DNA hybridization (Vandamme et al 1996).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of biochemical and molecular methods for Lactobacillus reuteri strains differentiation

et al. 2014

View full text Add to dashboard Cite

Several biochemical and molecular methods were used for discrimination of four Lactobacillus reuteri strains isolated from goatling and lamb stomach mucosa. Internal transcribed spacer (ITS)-PCR method and protein analysis by SDS-PAGE and MALDI-TOF showed to be suitable for strain discrimination whereas ITS-PCR/RFLP and enterobacterial repetitive intergenic consensus (ERIC)-PCR were not strain specific. The used methods differentiated tested strains into distinct groups; however, the location of strains in groups varied. Consistency in results was observed in the case of L. reuteri E and L. reuteri KO4m that were clustered into the same groups using all techniques, except of MALDI-TOF MS. The last one grouped goatling strains and lamb isolate into separate clusters. All investigated methods, except of ITS-PCR/RFLP and ERIC-PCR, were assessed as appropriate for distinguishing of L. reuteri strains.

show abstract

“…As well, it allows obtaining correct information on species affiliation and biodiversity of lactobacilli from different habitats. Recently, different L. helveticus strains isolated from Bulgarian yogurt were characterized according modern requirement by high discriminative molecular -typing methods (5). Molecular characterization and typing, both genetic and phenotypic, of probiotic strain Lactobacillus sp.…”

Section: Discussionmentioning

confidence: 99%

“…According to modern polyphasic taxonomy a combination of various phenotypic and molecular methods have to be used for typing and sub-typing of bacteria. Different molecular techniques, such as Randomly Amplified Polymorphic DNA (RAPD), Ribotyping, PCR -ribotyping, Restriction fragment length polymorphism (RFLP), multiplex PCR and PCR with specific primers, etc., have been applied to detect lactobacilli in various ecosystems (5,11). Amplified ribosomal DNA restriction analysis (ARDRA) is equally efficient for species level identification among Lactobacillus species of importance (12).…”

Section: Introductionmentioning

confidence: 99%

Molecular Taxonomic Characterisation of Probiotic StrainLactobacillus Sp.50P1

Nikolova

Evstatieva

Georgieva

et al. 2009

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

In recent years interest in the probiotic lactobacilli has been stimulated by the use of these bacteria in products that are claimed to confer health benefits on the consumer. The probiotic effects are usually strain-specific, meaning that a correct identification is important to link the strain to the specific health effect. Taxonomical characterization of probiotic strains only in phenotypic and phisyological characteristics is often with low level of discrimination, probably due to their co-evolution in the same ecological niches. Thus, the nucleotide base techniques provide an accurate basis for phylogenetic analysis and identification. With this aim a probiotic strain Lactobacillus spp. 50P1 was studied. Trough the physiological characterization with API 50 CH the strain 50P1, was determinate as Lactobacillus helveticus, with low probability-77,5%. Three PCR-based methods, species-specific PCR, of 16S rRNA gene sequencing, and restriction enzyme of 16S rDNA ARDRA analysis were used for reliable taxonomic characterization of probiotic strain Lactobacillus sp. 50P1. The sequence obtained from the strain was compared to those of Lactobacillus species held in GenBank and the belonging of the strain 50P1 to the species Lactobacillus helveticus was confirmed.

show abstract

Characterization of Lactobacillus helveticus strains isolated from Bulgarian yoghurt, cheese, plants and human faecal samples by sodium dodecilsulfate polyacrylamide gel electrophoresis of cell-wall proteins, ribotyping and pulsed field gel fingerprinting

Cited by 22 publications

References 16 publications

Multilocus restriction typing: A tool for studying molecular diversity within Lactobacillus helveticus of dairy origin

Multilocus restriction typing: A tool for studying molecular diversity within Lactobacillus helveticus of dairy origin

Evaluation of biochemical and molecular methods for Lactobacillus reuteri strains differentiation

Molecular Taxonomic Characterisation of Probiotic StrainLactobacillus Sp.50P1

Contact Info

Product

Resources

About