Characterization of lactic acid bacteria-based probiotics as potential heavy metal sorbents

Bhakta, Jatindra Nath; Ohnishi, Kouhei; Munekage, Yukihiro; Iwasaki, Kozo; Ma, Wei

doi:10.1111/j.1365-2672.2012.05284.x

Cited by 159 publications

(132 citation statements)

References 43 publications

(69 reference statements)

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“…The finding of lactobacillus binding to organophosphate pesticides is congruent with reports of similar interactions with environmental toxins, such as aflatoxin (24,25,39), paralytic shellfish toxins (37), and metals (38)(39)(40)(41)(42)(43).…”

Section: Figsupporting

confidence: 74%

Probiotic Lactobacillus rhamnosus Reduces Organophosphate Pesticide Absorption and Toxicity to Drosophila melanogaster

Trinder

McDowell

Daisley

et al. 2016

Appl Environ Microbiol

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Organophosphate pesticides used in agriculture can pose health risks to humans and wildlife. We hypothesized that dietary supplementation with Lactobacillus, a genus of commensal bacteria, would reduce absorption and toxicity of consumed organophosphate pesticides (parathion and chlorpyrifos [CP]). Several Lactobacillus species were screened for toleration of 100 ppm of CP or parathion in MRS broth based on 24-h growth curves. Certain Lactobacillus strains were unable to reach stationary-phase culture maxima and displayed an abnormal culture morphology in response to pesticide. Further characterization of commonly used, pesticide-tolerant and pesticide-susceptible, probiotic Lactobacillus rhamnosus strain GG (LGG) and L. rhamnosus strain GR-1 (LGR-1), respectively, revealed that both strains could significantly sequester organophosphate pesticides from solution after 24-h coincubations. This effect was independent of metabolic activity, as L. rhamnosus GG did not hydrolyze CP and no difference in organophosphate sequestration was observed between live and heat-killed strains. Furthermore, LGR-1 and LGG reduced the absorption of 100 M parathion or CP in a Caco-2 Transwell model of the small intestine epithelium. To determine the effect of sequestration on acute toxicity, newly eclosed Drosophila melanogaster flies were exposed to food containing 10 M CP with or without supplementation with live LGG. Supplementation with LGG simultaneously, but not with administration of CP 3 days prior (prophylactically), mitigated CP-induced mortality. In summary, the results suggest that L. rhamnosus may be useful for reducing toxic organophosphate pesticide exposure via passive binding. These findings could be transferable to clinical and livestock applications due to affordability and practical ability to supplement products with food-grade bacteria. IMPORTANCEThe consequences of environmental pesticide pollution due to widespread usage in agriculture and soil leaching are becoming a major societal concern. Although the long-term effects of low-dose pesticide exposure for humans and wildlife remain largely unknown, logic suggests that these chemicals are not aligned with ecosystem health. This observation is most strongly supported by the agricultural losses associated with honeybee population declines, known as colony collapse disorder, in which pesticide usage is a likely trigger. Lactobacilli are bacteria used as beneficial microorganisms in fermented foods and have shown potentials to sequester and degrade environmental toxins. This study demonstrated that commonly used probiotic strains of lactobacilli could sequester, but not metabolize, organophosphate pesticides (parathion and chlorpyrifos). This Lactobacillus-mediated sequestration was associated with decreased intestinal absorption and insect toxicity in appropriate models. These findings hold promise for supplementing human, livestock, or apiary foods with probiotic microorganisms to reduce organophosphate pesticide exposure.O rganophosphate pesticides are ...

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Section: Figsupporting

confidence: 74%

Probiotic Lactobacillus rhamnosus Reduces Organophosphate Pesticide Absorption and Toxicity to Drosophila melanogaster

Trinder

McDowell

Daisley

et al. 2016

Appl Environ Microbiol

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“…Of importance is the ability of lactobacilli to reduce oxidative stresses caused by metal toxicity in vitro (12,51) and detoxification abilities against other dietary toxins (104). The ability of lactobacilli to bind and sequester metals depends on the strain's resistance mechanisms.…”

Section: Microbial Mechanisms Of Actionmentioning

confidence: 99%

Bioremediation and Tolerance of Humans to Heavy Metals through Microbial Processes: a Potential Role for Probiotics?

Monachese

Burton

Reid

2012

Appl Environ Microbiol

272

142

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ABSTRACTThe food and water we consume are often contaminated with a range of chemicals and heavy metals, such as lead, cadmium, arsenic, chromium, and mercury, that are associated with numerous diseases. Although heavy-metal exposure and contamination are not a recent phenomenon, the concentration of metals and the exposure to populations remain major issues despite efforts at remediation. The ability to prevent and manage this problem is still a subject of much debate, with many technologies ineffective and others too expensive for practical large-scale use, especially for developing nations where major pollution occurs. This has led researchers to seek alternative solutions for decontaminating environmental sites and humans themselves. A number of environmental microorganisms have long been known for their ability to bind metals, but less well appreciated are human gastrointestinal bacteria. Species such asLactobacillus, present in the human mouth, gut, and vagina and in fermented foods, have the ability to bind and detoxify some of these substances. This review examines the current understanding of detoxication mechanisms of lactobacilli and how, in the future, humans and animals might benefit from these organisms in remediating environmental contamination of food.

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“…The metal(loid) removal percentage and removal efficiency were calculated using the equations described by Bhakta et al (2014) and Bhakta et al (2012b), respectively.…”

Section: Metal(loid) Biosorption Of Selected Resistant Isolatesmentioning

confidence: 99%

“…The metal(loid) biosorption study of the selected isolates was performed by measuring the Cd and As removal by the bacterial cells using methods described by Pazirandeh et al (1998) and Bhakta et al (2012b). Fresh bacterial cultures (24 h) were harvested in 2-ml centrifuge tubes by centrifugation (13,000g/min), and the cells were washed three times with sterilized Milli-Q (MQ) water.…”

Section: Metal(loid) Biosorption Of Selected Resistant Isolatesmentioning

confidence: 99%

Isolation and Characterization of Cadmium- and Arsenic-Absorbing Bacteria for Bioremediation

Bhakta

Munekage

Ohnishi

et al. 2014

Water Air Soil Pollut

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The contamination of hazardous metal(loid) is one of the serious environmental and human health risks. This study isolated a total of 40 cadmium (Cd)-and arsenic (As)-resistant bacterial isolates from coastal sediments by pour plate technique using tryptic soy agar supplemented with Cd or As (50 mg l −1 ) for use as metal(loid) bioremediation agents. Out of 40, 4 isolates, RCd3, RCd6, RAs7, and RAs10, showed a relatively higher growth rate in Cd-or As-supplemented culture media which were selected for further study. The selected isolates showed a high minimum inhibitory concentration (60-400 mg l −1 for Cd and 400-2200 mg l −1 for As), which demonstrated their remarkable Cd and As resistance capabilities. The metal(loid) removal efficiencies (0.032-0.268 μg Cd h −1 mg −1 and 0.0003-0.0172 μg As h −1 mg −1 [wet weight cell]) of selected isolates indicated their greater magnitude in absorbing Cd compared to As from water. Phylogenetic analysis of the 16S rDNA sequences revealed that isolates RCd3, RCd6, RAs7, and RAs10 were closely related to Acinetobacter brisouii, Pseudomonas abietaniphila, Exiguobacterium aestuarii, and Planococcus rifietoensis, respectively. Because of high Cd and As resistance and removal efficiency, the selected isolates can survive in a high metal(loid)-contaminated environment and could be a potential tool for bioremediation of high metal(loid)-contaminated effluents to protect the aquatic environment.

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Characterization of lactic acid bacteria-based probiotics as potential heavy metal sorbents

Cited by 159 publications

References 43 publications

Probiotic Lactobacillus rhamnosus Reduces Organophosphate Pesticide Absorption and Toxicity to Drosophila melanogaster

Probiotic Lactobacillus rhamnosus Reduces Organophosphate Pesticide Absorption and Toxicity to Drosophila melanogaster

Bioremediation and Tolerance of Humans to Heavy Metals through Microbial Processes: a Potential Role for Probiotics?

Isolation and Characterization of Cadmium- and Arsenic-Absorbing Bacteria for Bioremediation

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