Characterization of laboratory pyrimethanil-resistant mutants of Aspergillus flavus from groundnut in China

Liu, Ye; Liu, Zunyong; Hamada, M.; Yin, Yanni; Ma, Zhong H.

doi:10.1016/j.cropro.2014.02.004

Cited by 9 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mycelial plugs of activated strains 5 mm in diameter were used to inoculate the center of the PDA plates. After 8 d incubating at 25 °C in the dark, the colony diameter of each plate was measured in two perpendicular directions, subtracting the original inoculation plug (5 mm) . The 50% effective concentration (EC 50 ) was estimated from a regression curve between the percentage of inhibition and the log 10 -transfomed concentration of the fungicide …”

Section: Methodsmentioning

confidence: 99%

“…After 8 d incubating at 25 °C in the dark, the colony diameter of each plate was measured in two perpendicular directions, subtracting the original inoculation plug (5 mm). 21 The 50% effective concentration (EC 50 ) was estimated from a regression curve between the percentage of inhibition and the log 10 -transfomed concentration of the fungicide. 22 Effects of LNT Seed Dressing on Wheat Germination and Seedling Growth.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Use of Lentinan To Control Sharp Eyespot of Wheat, and the Mechanism Involved

Zhang¹,

Wang

Wang³

et al. 2017

J. Agric. Food Chem.

View full text Add to dashboard Cite

Lentinan (LNT), a complex polysaccharide with a β-(1→3)-linked backbone of d-glucose residues, has been reported to inhibit plant diseases. Our objective was to explore the efficacy and action mechanism of LNT used as a seed dressing to control sharp eyespot of wheat. Seed dressing promoted wheat growth. At control germination rates of 50%, 8 g of LNT/100 kg of seeds of the Jimai 22, Shannong 23, and Luyuan 502 cultivars significantly increased seed germination to 54%, 52%, and 51%, respectively. Seven days after emergence, the heights and root activity of wheat treated with LNT were significantly greater than those of controls. These effects were dose-dependent. At this time, the plant heights of Jimai 22, Shannong 23, and Luyuan 502 cultivars were 9.52, 8.52, and 10.52 cm, respectively, significantly higher than that of the controls. LNT prevented the development of wheat sharp eyespot. In the highly susceptible Jimai 22 cultivar, sharp eyespot development was reduced by 33.7%, 31.9%, and 30.4% at 7, 14, and 21 days after germination. LNT somewhat increased phenylalanine ammonia-lyase, peroxidase, and superoxide dismutase activity; reduced the malondialdehyde content; increased chlorophyll a and b levels; and enhanced the root vigor of wheat. These effects peaked 7 days after germination. LNT increased transcription of the genes encoding alternative oxidase (AOX) and β-1,3-glucanase (GLU), the salicylic acid signaling pathway-related gene NbPR1a, and the sharp eyespot resistance-related gene RS33. A significant dose-effect relationship was evident in terms of AOX transcription; we thus speculate that AOX may be the target gene.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Use of Lentinan To Control Sharp Eyespot of Wheat, and the Mechanism Involved

Zhang¹,

Wang

Wang³

et al. 2017

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…flavus and A. parasiticus infect peanut seeds and produce aflatoxins, which are harmful to both domestic animals and humans (Waliyar et al, 2015;Singh et al, 2015a,b;Liu et al, 2014). The progress in peanut breeding for resistance to aflatoxin is slow due to the lack of a cost-effective method for resistance identification in breeding materials or segregating progeny (Lei et al, 2006).…”

Section: Soil-borne Fungal Diseases and Associated Markersmentioning

confidence: 97%

Advancements in molecular marker development and their applications in the management of biotic stresses in peanuts

et al. 2015

View full text Add to dashboard Cite

“…Each treatment was repeated three times, and the culture was grown at 25 • C in dark. When the colony diameter of the control group reached approximately 70 mm, the diameter of the colonies was determined using the cross method (measuring the colony diameter in two vertical directions), and the average value was used to calculate the inhibition rate (%) of each concentration of the agent [31]. The data obtained were analyzed using SPSS 22.0 to calculate the EC 50 value of the agent against various pathogens.…”

Section: Fungicide Amended-agar Assaymentioning

confidence: 99%

A Polysaccharide of Ganoderma lucidum Enhances Antifungal Activity of Chemical Fungicides against Soil-Borne Diseases of Wheat and Maize by Induced Resistance

et al. 2022

View full text Add to dashboard Cite

Ganoderma lucidum polysaccharide (GLP), which is the primary active ingredient in G. lucidum, has been widely used in functional food and clinical medicine. However, it is rarely reported in the prevention and control of plant diseases. In this study, we found that the GLP can increase the germination rates and seedling heights of maize and wheat. We also found that the combination of GLP and chemical fungicides as a seed coating chemical compound has a control effect of more than 75% on the primary soil-borne diseases of the wheat and maize growing areas in both greenhouse and field trials. Furthermore, the combination of GLP and chemical fungicides prolongs the lasting period and reduces the application dosage of the chemical fungicides by half. In addition, GLP seed dressing could increase the resistance-related gene expression of the TPS and WRKY53 in maize and WMS533, NbPR1a, and RS33 in wheat. The combination of GLP and low-dose chemical fungicides proved to be an effective way to effectively prevent wheat sharp eyespot, root rot, and maize stalk rot in the wheat and maize continuous cropping areas in the North China Plain and to reduce pesticide use and increase crop yield.

show abstract

Characterization of laboratory pyrimethanil-resistant mutants of Aspergillus flavus from groundnut in China

Cited by 9 publications

References 31 publications

Use of Lentinan To Control Sharp Eyespot of Wheat, and the Mechanism Involved

Use of Lentinan To Control Sharp Eyespot of Wheat, and the Mechanism Involved

Advancements in molecular marker development and their applications in the management of biotic stresses in peanuts

A Polysaccharide of Ganoderma lucidum Enhances Antifungal Activity of Chemical Fungicides against Soil-Borne Diseases of Wheat and Maize by Induced Resistance

Contact Info

Product

Resources

About