Characterization of keratocalmin, a calmodulin-binding protein from human epidermis.

Fairley, Janet A.; Scott, Glynis; Jensen, Kasper; Goldsmith, Lowell A.; Díaz, Luis A.

doi:10.1172/jci115294

Cited by 17 publications

(1 citation statement)

References 38 publications

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“…Recently we have exploited immunological methods to confirm the presence of UCRP-protein conjugates within interferon-responsive cells (19,20 (Gibco) as previously described (2). In all experiments, interferon of the indicated type and concentration was added directly to the conditioned culture media 2 days after passage or medium change.…”

mentioning

confidence: 99%

Conjugates of ubiquitin cross-reactive protein distribute in a cytoskeletal pattern.

Loeb

Haas

1994

Mol. Cell. Biol.

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Ubiquitin cross-reactive protein (UCRP), a 15-kDa interferon-induced protein, is a sequence homolog of ubiquitin that is covalently ligated to intracellular proteins in a parallel enzymatic reaction and is found at low levels within cultured cell lines and human tissues not exposed to interferon. Ubiquitin and UCRP ligation reactions apparently target distinct subsets of intracellular proteins, as judged from differences in the distributions of the respective adducts revealed on immunoblots. In this study, successive passages of the human lung carcinoma line A549 in the presence of neutralizing antibodies against alpha and beta interferons had no effect on the levels of either free or conjugated UCRP, indicating that these UCRP pools are constitutively present within uninduced cells and are thus not a consequence of autoinduction by low levels of secreted alpha/beta interferon. In an effort to identify potential targets for UCRP conjugation, the immunocytochemical distribution of UCRP was examined by using affinity-purified polyclonal antibodies against recombinant polypeptide. UCRP distributes in a punctate cytoskeletal pattern that is resistant to extraction by nonionic detergents (e.g., Triton X-100) in both uninduced and interferon-treated A549 cells. The cytoskeletal pattern colocalizes with the intermediate filament network of epithelial and mesothelial cell lines. Immunoblots of parallel Triton X-100-insoluble cell extracts suggest that the cytoskeletal association largely results from the noncovalent association of UCRP conjugates with the intermediate filaments rather than direct ligation of the polypeptide to structural components of the filaments. A significant increase in the sequestration of UCRP adducts on intermediate filaments accompanies interferon induction. These results suggest that UCRP may serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments.

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mentioning

confidence: 99%

Conjugates of ubiquitin cross-reactive protein distribute in a cytoskeletal pattern.

Loeb

Haas

1994

Mol. Cell. Biol.

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The molecular biology of desmosomes and hemidesmosomes: ′What's in a name?'

1992

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Desmosomes are junctions involved in intercellular adhesion of epithelial cells and hemidesmosomes are junctions involved in adhesion of epithelia to basement membranes. Both are characterised at the ultrastructural level by dense cytoplasmic plaques which are linked to the intermediate filament cytoskeleton of the cells. The plaques strongly resemble each other suggesting a relationship between the two kinds of junctions, as implied by their names. Recent characterisation of the molecular components of the junctions shows they are, in fact, quite unrelated implying that structural similarity is fortuitous. The molecular biology raises many fascinating problems relating to their structure and function.

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Structural and Functional Regulation of Desmosomes

Getsios¹,

Godsel²,

Green³

Rise and Fall of Epithelial Phenotype

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Characterization of keratocalmin, a calmodulin-binding protein from human epidermis.

Abstract: Using affinity-purified calmodulin-binding proteins from human epidermis we have developed a monoclonal IgM antibody, ROC 129

Cited by 17 publications

References 38 publications

Conjugates of ubiquitin cross-reactive protein distribute in a cytoskeletal pattern.

Conjugates of ubiquitin cross-reactive protein distribute in a cytoskeletal pattern.

The molecular biology of desmosomes and hemidesmosomes: ′What's in a name?'

Structural and Functional Regulation of Desmosomes

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