Characterization of Jordanian olive cultivars (Olea europaea L.) using RAPD and ISSR molecular markers

Brake, Mohammad; Migdadi, Hussein M.; Al-Gharaibeh, Moath; Ayoub, Salam; Haddad, Nizar; Oqlah, Ahmad El

doi:10.1016/j.scienta.2014.07.012

Cited by 32 publications

(22 citation statements)

References 37 publications

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“…Therefore, there is a great need for wholesalers, retailers and consumers to know the genetic relationship of C. nutans in different populations. Besides that, information on genetic diversity of C. nutans can be useful in developing agricultural practices that can avoid genetic erosion and can offer conservation strategies for the long-term vigour of the species (Brake et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of genetic diversity of Clinacanthus nutans (Acanthaceaea) using RAPD, ISSR and RAMP markers

Ismail

Arsad

Samian

et al. 2016

Physiol Mol Biol Plants

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Three polymerase chain reaction (PCR) techniques were compared to analyse the genetic diversity of Clinacanthus nutans eight populations in the northern region of Peninsular Malaysia. The PCR techniques were random amplified polymorphic deoxyribonucleic acids (RAPD), inter-simple sequence repeats (ISSR) and random amplified microsatellite polymorphisms (RAMP). Leaf genomic DNA was PCR amplified using 17 RAPD, 8 ISSR and 136 RAMP primers . However, only 10 RAPD primers, 5 ISSR primers and 37 RAMP primers produced reproducible bands. The results were evaluated for polymorphic information content (PIC), marker index (MI) and resolving power (RP). The RAMP marker was the most useful marker compared to RAPD and ISSR markers because it showed the highest average value of PIC (0.25), MI (11.36) and RP (2.86). The genetic diversity showed a high percentage of polymorphism at the species level compared to the population level. Furthermore, analysis of molecular variance revealed that the genetic diversity was higher within populations, as compared to among populations of C. nutans. From the results, the RAMP technique was recommended for the analysis of genetic diversity of C. nutans.

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Section: Introductionmentioning

confidence: 99%

Evaluation of genetic diversity of Clinacanthus nutans (Acanthaceaea) using RAPD, ISSR and RAMP markers

Ismail

Arsad

Samian

et al. 2016

Physiol Mol Biol Plants

View full text Add to dashboard Cite

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“…The use of RAPD markers has been extremely common, alone or in combination with other markers (AFLPs, ISSRs, SSRs), to analyse the extent of genetic variability present in the germplasm of varieties of countries characterised by an old history of olive cultivation and oil production: Malta (Mazzitelli et al 2014), Jordan (Brake et al 2014), Greece (Linos et al 2014), Egypt (Elsheikh et al 2014), Iran (Sheidai et al 2014), Pakistan (Awan et al 2011), and Turkey (Coskun and Parlak 2013). In other papers, RAPD markers have been considered to study the potential presence of intra-cultivar variability (Ç elikkol Akçay et al 2014;Figueiredo et al 2013;Leva and Petruccelli 2012), the genetic relationships between cultivars (Gomes et al 2012), the genetic characterisation of minor cultivars in local regions (Parra-Lobato et al 2012), the results of in vitro mutagenesis, and the detection of possible mutants in olive calli caused by the use of sodium azide (Alborzian Deh Sheikh and Moradnejad 2014).…”

Section: Rapds Issrs and Aflpsmentioning

confidence: 99%

“…In addition to other markers, ISSR have been used to study and characterise the genetic variability in local olive germplasm (Zhan et al 2015;Brake et al 2014;Elsheikh et al 2014;Linos et al 2014;Leva and Petruccelli 2012;Noormohammadi et al 2012) Further, Kaya and Yilmaz-Gokdogan (2016) have used ISSRs in addition to two retrotransposon-based marker systems (IRAPinter-retrotransposon amplified polymorphism and REMAPretrotransposon-microsatellite amplified polymorphism) for the molecular characterisation of olive cultivars.…”

Section: Rapds Issrs and Aflpsmentioning

confidence: 99%

Recent developments in olive (Olea europaea L.) genetics and genomics: applications in taxonomy, varietal identification, traceability and breeding

Sebastiani

Busconi

2017

Plant Cell Rep

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The latest results in DNA markers application and genomic studies in olive. Olive (Olea europaea L.) is among the most ancient tree crops worldwide and the source of oil beneficial for human health. Despite this, few data on olive genetics are available in comparison with other cultivated plant species. Molecular information is mainly linked to molecular markers and their application to the study of DNA variation in the Olea europaea complex. In terms of genomic research, efforts have been made in sequencing, heralding the era of olive genomic. The present paper represents an update of a previous review work published in this journal in 2011. The review is again mainly focused on DNA markers, whose application still constitutes a relevant percentage of the most recently published researches. Since the olive genomic era has recently started, the latest results in this field are also being discussed.

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“…102 Either seven ISSR or 12 RAPD primers were able to distinguish 38 cultivars, mainly from Portugal. 102 The combination of ISSRs and RAPDs also allowed to characterize Jordanian cultivars 103 and to monitor cultivar identity in micro-propagated olive plants (cv. Maurino), 104 as well as to point out the relationships between olive accessions from Madeira and the Canary Islands.…”

Section: Combined Use Of Different Dna Markersmentioning

confidence: 99%

Evolution and perspectives of cultivar identification and traceability from tree to oil and table olives by means of DNA markers

et al. 2016

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In recent years, an increasing number of typicality marks has been awarded to high-quality olive oils produced from local cultivars. In this case, quality control requires effective varietal checks of the starting materials. Moreover, accurate cultivar identification is essential in vegetative-propagated plants distributed by nurseries and is a pre-requisite to register new cultivars. Food genomics provides many tools for cultivar identification and traceability from tree to oil and table olives. The results of the application of different classes of DNA markers to olive with the purpose of checking cultivar identity and variability of plant material are extensively discussed in this review, with special regard to repeatability issues and polymorphism degree. The characterization of olive germplasm from all countries of the Mediterranean basin and from less studied geographical areas is described and innovative high-throughput molecular tools to manage reference collections are reviewed. Then the transferability of DNA markers to processed products - virgin olive oils and table olives - is overviewed to point out strengths and weaknesses, with special regard to (i) the influence of processing steps and storage time on the quantity and quality of residual DNA, (ii) recent advances to overcome the bottleneck of DNA extraction from processed products, (iii) factors affecting whole comparability of DNA profiles between fresh plant materials and end-products, (iv) drawbacks in the analysis of multi-cultivar versus single-cultivar end-products and (v) the potential of quantitative polymerase chain reaction (PCR)-based techniques. © 2016 Society of Chemical Industry.

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Characterization of Jordanian olive cultivars (Olea europaea L.) using RAPD and ISSR molecular markers

Cited by 32 publications

References 37 publications

Evaluation of genetic diversity of Clinacanthus nutans (Acanthaceaea) using RAPD, ISSR and RAMP markers

Evaluation of genetic diversity of Clinacanthus nutans (Acanthaceaea) using RAPD, ISSR and RAMP markers

Recent developments in olive (Olea europaea L.) genetics and genomics: applications in taxonomy, varietal identification, traceability and breeding

Evolution and perspectives of cultivar identification and traceability from tree to oil and table olives by means of DNA markers

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