Characterization of Japanese flounder (Paralichthys olivaceus) NK-lysin, an antimicrobial peptide

Hirono, Ikuo; Kondo, Hidehiro; Koyama, Takashi; Arma, Nur Rahmawaty; Hwang, Jee Youn; Nozaki, Reiko; Midorikawa, Naoko; Aoki, Takashi

doi:10.1016/j.fsi.2006.08.003

Cited by 59 publications

(37 citation statements)

References 29 publications

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“…The gene expression characteristics as well as the antibacterial activities of its synthetic peptides (Lc-NK-lysin-1 and Lc-NK-lysin-2) were also tested. The DNA sequence of Lc-NK-lysin gene contained five exons and four introns, which was accord with the feature of all reported NK-lysin genes in fishes [11,13,21], and it may suggest that the NK-lysin genes have common structure. The Saposin B domain and the six well conserved cysteine residues were the typical characteristic of NKlysin peptides, which were speculated related with its biological activities [13].…”

Section: Lc-nk-lysin: a Typical Member Of The Nk-lysin Familysupporting

confidence: 82%

“…The three types of NK-lysin gene found in channel catfish were mainly expressed in gill, head kidney and spleen, while the expressions were extremely low or not detected in skin, stomach, and muscle [21]. The NK-lysin gene from Japanese flounder was highly expressed in gill, heart, head kidney and spleen, slightly expressed or no expression in liver, muscle, brain and stomach, which is similar to the expression pattern in mammals [11]. The NK-lysin gene from the half-smooth tongue sole was also most detectable in head kidney and spleen, but not detected in muscle [13].…”

Section: Expression Patterns Of Lc-nk-lysin Genementioning

confidence: 50%

“…Based on the secondary structure of NK-lysin mature polypeptide sequences in reported fishes [11,13,21], two peptides (Lc-NK-lysin-1 and Lc-NK-lysin-2) from the core region of Lc-NK-lysin mature polypeptide were identified and synthesized. The antibacterial activities assay showed that the two peptides exhibited antibacterial activities to some of the bacteria, especially to the non-marine species, and the antibacterial activities of Lc-NK-lysin-1 were slightly stronger than Lc-NK-lysin-2.…”

Section: Antibacterial Activities Of Lc-nk-lysin Peptidesmentioning

confidence: 99%

“…In teleosts, NK-lysin was found in channel catfish (Ictalurus punctatus) [10], Japanese flounder (Paralichthys olivaceus) [11], halfsmooth tongue sole (Cynoglossus semilaevis) [13] and zebrafish (Danio rerio) [18]. Even though NK-lysin has been identified and described in teleosts, the studies on its immune responses and antibacterial activities were still insufficient.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Identification, expression and antibacterial activities of an antimicrobial peptide NK-lysin from a marine fish Larimichthys crocea

Zhou

Wang

Liu

et al. 2016

Fish & Shellfish Immunology

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Section: Lc-nk-lysin: a Typical Member Of The Nk-lysin Familysupporting

confidence: 82%

Section: Expression Patterns Of Lc-nk-lysin Genementioning

confidence: 50%

Section: Antibacterial Activities Of Lc-nk-lysin Peptidesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification, expression and antibacterial activities of an antimicrobial peptide NK-lysin from a marine fish Larimichthys crocea

Zhou

Wang

Liu

et al. 2016

Fish & Shellfish Immunology

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“…However, there are only few reports on the antibacterial activity of NK-lysins from aquatic species. Only the Japanese flounder NK-lysin proteins have been identified to have antibacterial activity against various Gram-negative bacteria [29]. In the current study, although three distinct mNKLs all consisted of 76 amino acids, they showed diversity in amino acid sequences.…”

Section: Discussionmentioning

confidence: 57%

Expression, Purification and Antibacterial Activity of NK-Lysin Mature Peptides from the Channel Catfish (Ictalurus punctatus)

Cai

Wang

et al. 2016

Applied Sciences

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Antimicrobial peptides (AMPs) are small peptides and play important roles in host innate immune response against microbial invasion. Aquatic animals secrete different kinds of antimicrobial peptides which have antimicrobial activity towards microorganisms. NK-lysins, mature peptides produced by cytotoxic T lymphocytes and natural killer cells, are comprised of 74-78 amino acid residues, demonstrating broad-spectrum antimicrobial activity against bacteria, fungi, protozoa, and parasites. In this study, three distinct NK-lysin mature peptide (mNKLs), transcripts (76 amino acid residues) cloned from the channel catfish (Ictalurus punctatus) head kidney were ligated into plasmid vector pET-32a(+) to express the mNKLs fusion protein. The fusion protein was successfully expressed in E. coli Rosetta (DE3) under optimized conditions. After purification by affinity column chromatography, the fusion protein was successfully cleaved by enterokinase and released the peptide mNKLs. Tricine-SDS-PAGE results showed that mNKLs (approximately 8.6 kDa) were successfully expressed. The purified peptide mNKLs exhibited antibacterial activity against Staphylococcus aureus and E. coli.

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Expression profiles of four Nile Tilapia innate immune genes during early stages of Aeromonas veronii infection

Bakry,

Emeish,

Embark

et al. 2024

J Aqua Anim Hlth

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ObjectiveDuring Egypt's hot summer season, Aeromonas veronii infection causes catastrophic mortality on Nile Tilapia Oreochromis niloticus farms. Egypt is ranked first in aquaculture production in Africa, sixth in aquaculture production worldwide, and third in global tilapia production. This study aimed to investigate, at the molecular level, the early innate immune responses of Nile Tilapia to experimental A. veronii infection.MethodsThe relative gene expression, co‐expression clustering, and correlation of four selected immune genes were studied by quantitative real‐time polymerase chain reaction in four organs (spleen, liver, gills, and intestine) for up to 72 h after a waterborne A. veronii challenge. The four genes studied were nucleotide‐binding oligomerization domain 1 (NOD1), lipopolysaccharide‐binding protein (LBP), natural killer‐lysin (NKL), and interleukin‐1 beta (IL‐1β).ResultThe four genes showed significant transcriptional upregulation in response to infection. At 72 h postchallenge, the highest NOD1 and IL‐1β expression levels were recorded in the spleen, whereas the highest LBP and NKL expression levels were found in the gills. Pairwise distances of the data points and the hierarchical relationship showed that NOD1 clustered with IL‐1β, whereas LBP clustered with NKL; both genes within each cluster showed a significant positive expression correlation. Tissue clustering indicated that the responses of only the gill and intestine exhibited a significant positive correlation.ConclusionThe results suggest that NOD1, LBP, NKL, and IL‐1β genes play pivotal roles in the early innate immune response of Nile Tilapia to A. veronii infection, and the postinfection expression profile trends of these genes imply tissue‐/organ‐specific responses and synchronized co‐regulation.

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Characterization of Japanese flounder (Paralichthys olivaceus) NK-lysin, an antimicrobial peptide

Cited by 59 publications

References 29 publications

Identification, expression and antibacterial activities of an antimicrobial peptide NK-lysin from a marine fish Larimichthys crocea

Identification, expression and antibacterial activities of an antimicrobial peptide NK-lysin from a marine fish Larimichthys crocea

Expression, Purification and Antibacterial Activity of NK-Lysin Mature Peptides from the Channel Catfish (Ictalurus punctatus)

Expression profiles of four Nile Tilapia innate immune genes during early stages of Aeromonas veronii infection

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