Characterization of Japanese eel immunoglobulin M and its level in serum

Uchida, Daisuke; Chang, Pei Kan; Aranishi, Futoshi; Hirayabu, Eiji; Mano, Nobuhiro; Mitsuya, T; Prayitno, Slamet Budi; Natori, Masahiko

doi:10.1016/s0305-0491(00)00290-x

Cited by 30 publications

(15 citation statements)

References 30 publications

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“…The soluble form is secreted from B cells and is present in the blood and other fluids, where it plays a role as an immune effector molecule (Ross et al 1998). Measurements of IgM levels in serum from several fish species have been surveyed and the results point to a range between 0.25 and 23.5 mg/ml (Israelson et al 1991;Magnadottir and Gudmundsdottir, 1992;Uchida et al 2000;Shelby et al 2002). The results of our study are in agreement with the results of previous investigations.…”

Section: Discussionsupporting

confidence: 83%

Immune Response of Rainbow Trout (Oncorhynchus mykiss) to Selected Antigens of Yersinia ruckeri

et al. 2009

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In this study, effects of Yersinia ruckeri antigens on the immune mechanisms of rainbow trout (Oncorhynchus mykiss) were examined. The weight of the 120 fish used in this study was 20-30 g. After injecting 1 mg of formalin-inactivated whole cells (FKC) and O-antigen (Ag-O) intraperitoneally, blood was taken from the caudal vein of anaesthetized fish and metabolic activity of leukocytes (Nitroblue tetrazolium (NBT) activities), phagocytic activity (PA), phagocytic index (PI), serum protein and serum total immunoglobulin (TIg) levels were determined on day 30 after the first immunization. The same procedure was conducted in the control group. In all the experimental groups, considerable increases in the immune indicators were found and significant differences detected between the control and experimental groups (p < 0.05). Metabolic activity of leukocytes decreased significantly (p < 0.05) during the following treatment with antigens compared to the control fish group.

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Section: Discussionsupporting

confidence: 83%

Immune Response of Rainbow Trout (Oncorhynchus mykiss) to Selected Antigens of Yersinia ruckeri

et al. 2009

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“…This form of structural diversity, which has also been reported in rat IgA (8) and human IgG 4 (9), can be easily observed when performing denaturing, nonreducing electrophoresis. Under these conditions whole native tetramers (teleost IgM), dimers (rat IgA), or monomers (human IgG 4 ) can be resolved into smaller disulfide polymerized subunits (1)(2)(3)(4)(5)(6)(7)(8)(9). In contradistinction with past work on mammalian IgM (10), it would appear that stringency in the complete disulfide polymerization of IgM is obviously not as critical for teleosts as it may be in mammals.…”

mentioning

confidence: 62%

“…V ariability in the degree of interheavy chain disulfide polymerization within the tetrameric teleost IgM molecule has been observed in .15 species (1)(2)(3)(4)(5)(6)(7). This form of structural diversity, which has also been reported in rat IgA (8) and human IgG 4 (9), can be easily observed when performing denaturing, nonreducing electrophoresis.…”

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confidence: 99%

The Strength of B Cell Interaction with Antigen Determines the Degree of IgM Polymerization

Bromage

Kaattari

2009

The Journal of Immunology

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The induction of variable disulfide polymerization of IgM in the trout (Oncorhynchus mykiss) and its effect on its half-life were examined. An association between greater Ab affinity and increased disulfide polymerization was first indicated by the observation of this increased IgM disulfide polymerization during the process of affinity maturation. A direct association between Ab affinity and disulfide polymerization was then established by the fractionation of individual sera into high- and low-affinity subpopulations, which also resulted in the partitioning of high and low degrees of disulfide polymerization. The ability of high-affinity B cells to produce more highly polymerized Abs upon Ag induction was demonstrated by in vitro Ag-driven selection. Low Ag concentrations, which elicited only high-affinity Abs, also possessed the highest degree of polymerization, whereas higher concentrations of Ag elicited a broader array of Ab affinities, yielding a lower average affinity and degree of polymerization. Half-life studies revealed that the high-affinity, highly polymerized Abs possessed longer half-lives than the lower-affinity, lightly polymerized Abs. Finally, although the affinity for Ag is associated with elevated levels of polymerization, analysis of naive Ig revealed that the degree of polymerization alone, not affinity, appears sufficient to prolong Ig half-life.

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“…In this context, adequate knowledge about the structure and character of the immunoglobulin of IMC provides a strong base for development of diagnostics and vaccines. There are several studies on characterization of the immunoglobulin (Ig) of commercial fishes such as Japanese eel [7], Snapper [8], Rainbow trout [9], European Sea bass [10], Common carp [11,12], Turbot [13], African catfish [14], Cod [15], Atlantic salmon [16], Tilapia [17], Channel catfish [18] and Brown bullhead [19]. The predominant antibody type in fish is high molecular weight Ig often referred to as IgM or IgM like because this molecule has a heavy chain isotype similar to the mammalian m chain [20].…”

mentioning

confidence: 99%

Isolation and characterization of immunoglobulin of the Indian major carp, rohu [Labeo rohita (Ham.)]

Babu

Shankar

Honnananda

et al. 2008

Fish & Shellfish Immunology

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Characterization of Japanese eel immunoglobulin M and its level in serum

Cited by 30 publications

References 30 publications

Immune Response of Rainbow Trout (Oncorhynchus mykiss) to Selected Antigens of Yersinia ruckeri

Immune Response of Rainbow Trout (Oncorhynchus mykiss) to Selected Antigens of Yersinia ruckeri

The Strength of B Cell Interaction with Antigen Determines the Degree of IgM Polymerization

Isolation and characterization of immunoglobulin of the Indian major carp, rohu [Labeo rohita (Ham.)]

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