Characterization of ion exchange stationary phases via pH transition profiles

Lendero, Nika; Vidič, Jana; Brne, Peter; Frankovič, Vida; Štrancar, Aleš; Podgornik, Aleš

doi:10.1016/j.chroma.2008.01.023

Cited by 36 publications

(21 citation statements)

References 15 publications

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“…This method is based on the titration of ion‐exchange functional groups immobilized on the monolith pore surface with two buffers having the same pH value but different salt concentrations as commonly implemented in IEC. A stepwise change in the buffer results in a pH transient curve, the duration of which depends on the amount of functional groups on the matrix (ion‐exchange capacity) . Typical pH transient curves are shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Effect of pore size on performance of monolithic tube chromatography of large biomolecules

et al. 2017

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Effect of pore size on the performance of ion-exchange monolith tube chromatography of large biomolecules was investigated. Radial flow 1 mL polymer based monolith tubes of different pore sizes (1.5, 2, and 6 μm) were tested with model samples such as 20 mer poly T-DNA, basic proteins, and acidic proteins (molecular weight 14 000-670 000). Pressure drop, pH transient, the number of binding site, dynamic binding capacity, and peak width were examined. Pressure drop-flow rate curves and dynamic binding capacity values were well correlated with the nominal pore size. While duration of the pH transient curves depends on the pore size, it was found that pH duration normalized on estimated surface area was constant, indicating that the ligand density is the same. This was also confirmed by the constant number of binding site values being independent of pore size. The peak width values were similar to those for axial flow monolith chromatography. These results showed that it is easy to scale up axial flow monolith chromatography to radial flow monolith tube chromatography by choosing the right pore size in terms of the pressure drop and capacity.

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Section: Resultsmentioning

confidence: 99%

Effect of pore size on performance of monolithic tube chromatography of large biomolecules

et al. 2017

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“…For cationic systems, a = 1.57 and b = 0.5. These constants were previously calculated by Nika Lendero et al [32] for similar systems.…”

Section: Determination Of the Ionic Capacitymentioning

confidence: 97%

“…The time interval t(pH) recorded between the switching point of the mobile phase and the point at which 50% of the maximum reachable pH value was actually obtained was recorded. The ionic capacity was calculated from the relative elution volumes, normalized by column volume, as described [31,32] with the following expression:…”

Section: Determination Of the Ionic Capacitymentioning

confidence: 99%

Preparation, characterization, and process performance of composite fibrous adsorbents as cation exchangers for high throughput and high capacity bioseparations

Gavara

Cabrera

Vennapusa

et al. 2012

Journal of Chromatography B

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“…The time interval ∆t (pH) was determined between the switching point of the mobile phase and the point at which 50% of the maximum pH value was achieved. The phosphate capacity was calculated using Equation (3), where the volumetric flow rate (ϕv), concentration of elution buffer solution (C2), and column volume (Vc), as well as time interval (∆t) are used in the expression [50,51]. Q Sepharose FF beads were utilized as a control ion exchange sample.…”

Section: Determination Of the Ionic Capacitymentioning

confidence: 99%

Chromatographic Characterization and Process Performance of Column-Packed Anion Exchange Fibrous Adsorbents for High Throughput and High Capacity Bioseparations

et al. 2015

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Fibrous materials are prominent among novel chromatographic supports for the separation and purification of biomolecules. In this work, strong anion exchange, quaternary ammonium (Q) functional fibrous adsorbents were evaluated with regards to their physical and functional characteristics. A column packed with Q fibrous adsorbent illustrated the good column packing efficiency of theoretical plate height (H) values and higher permeability coefficients (>0.9 × 10 −7 cm 2 ) than commercial adsorbents. For pulse experiments with acetone and lactoferrin as tracers under nonbinding conditions, the total porosity (for acetone) and the interstitial porosity (for lactoferrin) measured 0.97 and 0.47, respectively. The total ionic capacity of the chemically-functionalized Q fiber was 0.51 mmol/mL. The results indicated that the Q fiber had a static binding capacity of 140 mg/mL and a dynamic binding capacity (DBC) of 76 mg/mL for bovine serum albumin (BSA) and showed a linearly-scalable factor (~110 mL) for a column volume with high capacity and high throughput. Furthermore, this adsorptive material had the ability to bind OPEN ACCESSProcesses 2015, 3 205 the high molecular weight protein, thyroglobulin, with a capacity of 6 mg/mL. This work demonstrated the column-packed Q fibrous adsorption system as a potential chromatography support that exhibits high capacity at higher flow rates.

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Characterization of ion exchange stationary phases via pH transition profiles

Cited by 36 publications

References 15 publications

Effect of pore size on performance of monolithic tube chromatography of large biomolecules

Effect of pore size on performance of monolithic tube chromatography of large biomolecules

Preparation, characterization, and process performance of composite fibrous adsorbents as cation exchangers for high throughput and high capacity bioseparations

Chromatographic Characterization and Process Performance of Column-Packed Anion Exchange Fibrous Adsorbents for High Throughput and High Capacity Bioseparations

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