Characterization of ion channel and transporter mRNA expressions in isolated gill chloride and pavement cells of seawater acclimating eels

Tse, William Ka Fai; Au, Doris W.T.; Wong, Chris K C

doi:10.1016/j.bbrc.2006.06.028

Cited by 115 publications

(90 citation statements)

References 26 publications

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“…The methodologies employed for isolation and fractionation of cells from the gills of the freshwater bivalve L. costata and the seawater clam M. mactroides were found to be effective, as previously reported for fish (Goss et al 2001;Galvez et al 2002;Tse et al 2006). Also, the application of two different approaches (mitochondria density and Na ?…”

Section: Resultssupporting

confidence: 64%

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Isolation and fractionation of gill cells from freshwater (Lasmigona costata) and seawater (Mesodesma mactroides) bivalves for use in toxicological studies with copper

Nogueira¹,

Wood

Gillis

et al. 2013

Cytotechnology

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Gills cells of the freshwater mussel Lasmigona costata and the seawater clam Mesodesma mactroides were isolated (mussel: chemical dissociation; clam: mechanical dissociation) and fractionated (Percoll gradient) into Fractions I and II. Mitochondrial dyes (DASPEI: mussel; MitoTracker Ò : clam) and Na ? , K ? -ATPase activity measurement were used to distinguish between cells of Fractions I and II. For mussel and clam, 80.5 ± 1.5 and 48.3 ± 3.2 % of cells were in Fraction II, respectively. For both species, cells of Fraction II had higher fluorescence emission and higher enzyme activity than those of Fraction I, being characterized as 'cells rich in mitochondria'. Cells of Fraction II were kept in saline solutions approximating the ionic composition of hemolymph either under control conditions (no Cu addition) or exposed (3 h) to copper (Cu: 5, 9 and 20 lg Cu/L). Cell viability and Cu and Na ? content were measured. For both species, Cu content was higher and Na ? content was lower in cells exposed to 20 lg Cu/L. Furthermore, a strong negative correlation was observed between cell Na ? and Cu content in the two bivalve species, indicating a possible competition between Cu and Na ? for ion-transporting mechanisms or binding sites at gill cells of Fraction II. Considering that Cu is an ionoregulatory toxicant in aquatic invertebrates, these preliminary toxicological data support the idea of using isolated gill cells rich in mitochondria to study the mechanisms underlying the acute toxicity of waterborne Cu in freshwater and marine bivalves.

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Section: Resultssupporting

confidence: 64%

“…, K ? -ATPase activity measurement, as earlier applied for teleost fish Kelly et al 2000;Wong and Chan 2001;Tse et al 2006). Two distinct fractions were identified, one of which (Fraction II) was rich in mitochondria and Na ?…”

Section: Introductionmentioning

confidence: 96%

Isolation and fractionation of gill cells from freshwater (Lasmigona costata) and seawater (Mesodesma mactroides) bivalves for use in toxicological studies with copper

Nogueira¹,

Wood

Gillis

et al. 2013

Cytotechnology

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“…This mechanism showed that every change in Na + , K + -ATPase activity and abundance can be affected by NKCC cotransporter (Pelis and McCormick, 2001). Increasing NKCC gene expression after acclimation to salinity was previously reported in Anguilla japonica (Tse and Wong, 2006), Dicentrarchus labrax (acclimated to 36 ppt salinity) (Lorin-Nebel et al, 2006), Anguilla Anguilla and Fundulus heteroclitus (acclimated to 35 ppt salinity) (Scott et al, 2004;Scott and Schulte, 2005). In our study, gill NKCC gene expression increased significantly in 5 ppt-acclimated fish in comparison with the freshwater group.…”

Section: Nkcc Gene Expressionsupporting

confidence: 58%

“…Whereas prolactin has been established as an important hormone for the maintenance of ionic homeostasis in freshwater, growth hormone (GH) and cortisol are recognised as the major seawater-adapting hormones (Madsen, 1990a and1990b;McCormick, 1995;Kiilerich et al, 2007). Many studies have shown that changes in salinity and hormones such as cortisol, growth hormone (GH) and prolactin (PRL) can stimulate expression and activity of different ion and electrolyte transporters in the ionocytes, such as NKA and NKCC (Pelis and McCormick, 2001;Mancera et al, 2002;Singer et al, 2003;Lin et al, 2004;Tse et al, 2006;).…”

Section: Introductionmentioning

confidence: 99%

Effects of cortisol and salinity acclimation on Na+/K+/2Cl–- cotransporter gene expression and Na+, K+-ATPase activity in the gill of Persian sturgeon, Acipenser persicus, fry

Khodabandeh¹,

Mosafer²,

Khoshnood³

2009

Sci. Mar.

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SUMMARY: Na + , K + -ATPase activity and Na + /K + /2Cl -cotransporter (NKCC) gene expression in the gills of Persian sturgeon, Acipenser persicus, fry (2-3 g, 3.30-8.12 cm total body length) in freshwater (control group), diluted Caspian Sea water (5 ppt) and after treatment with cortisol in freshwater were studied. Na + , K + -ATPase activity was lower in the 5 pptacclimated fish (1.07±0.05 µmol P i /mg protein/h) than in the control fish (1.19±0.05 µmol P i /mg protein/h) but this difference was not significant. NKCC gene expression in the 5 ppt-acclimated fish (1.6±0.07) was significantly higher than in the control fish (0.8±0.00). In the cortisol treated fish, Na + , K + -ATPase activity (1.91±0.05 µmol P i /mg protein/h) and NKCC gene expression (3.2±0.1) were significantly higher than in the control group. Our results show that Persian sturgeon fry (2-3 g) can tolerate 5 ppt salinity by changing their enzymatic content and activity, and that exogenous cortisol application can increase the osmoregulatory capacity of fry before release into brackish water and can reduce their mortality.Keywords: cortisol, Na + , K + -ATPase, NKCC, Persian sturgeon, salinity. -Se estudió la actividad Na + , K + -ATPasa y el cotransportador de expresión génica (NKCC) Na + /K + /2Cl -en las branquias de juveniles de esturión de Persia, Acipenser persicus, (2-3 g, 3.30-8.12 cm de longitud total) en agua dulce (grupo control), agua diluida del mar Caspio (5 ppt) y posterior tratamiento con cortisol en agua dulce. La actividad Na + , K + -ATPasa fue menor en los peces aclimatados en 5 ppt (1.07±0.05 µmol P i /mg proteína/h) que en los peces (1.19±0.05 µmol P i /mg proteína/h), pero esta diferencia no fue significativa. La expresión génica NKCC en peces desde 5 ppt de salinidad (1.6±0.07) fue significativamente más alta que en el grupo (0.8±0.00). En los peces tratados con cortisol la actividad Na + , K + -ATPasa (1.91±0.05 µmol P i /mg proteína/h) y la expresión génica NKCC (3.2±0.1) incrementaron significativamente en comparación con el grupo control. Nuestros resultados mostraron que los juveniles de esturión de Persia (2-3 g) pueden tolerar 5 ppt de salinidad y esta capacidad se consiguió cambiando su contenido y actividad enzimática; -la aplicación de cortisol exógeno puede incrementar la capacidad osmoregulatoria de los juveniles antes de soltarlos a aguas salobres y puede reducir su mortalidad. RESUMEN: EFECTOS DE

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“…A number of key hormones (growth hormone, cortisol, prolactin, natriuretic peptides, and stanniocalcin 1 (STC1)) have been identified that are essential in coordinating gill activity. Over the past decade, a considerable number of studies have investigated how ion-osmoregulatory function associates with changes in plasma hormone levels and with the regulation of the expression of different ion transporters/channels in gill cells (Perry 1997, Wong & Chan 1999a,b, Evans 2002, Evans et al 2005, Tse et al 2006. Different models of ion transport in gill cells of both freshwater (FW) and seawater fish and the functional roles of the respective hormones have been proposed and summarized in recent reviews (Hwang et al 2011, Takei et al 2014.…”

Section: Introductionmentioning

confidence: 99%

Characterization of stanniocalcin 1 binding and signaling in gill cells of Japanese eels

Gu¹,

Law²,

Yeung³

et al. 2015

Journal of Molecular Endocrinology

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Stanniocalcin 1 (STC1) is a hypocalcemic hormone that is known to play an important role in calcium metabolism in teleost fish. An increase in blood Ca 2C levels stimulates its synthesis and release. . Our results indicated that STC1 exerted its inhibitory action via a cAMP pathway to lower intracellular Ca 2C levels.Intriguingly, we were able to block the action of STC1 using an inhibitor, NS-398, of cyclooxygenase-2 (COX-2), which is known to stimulate the activity of sarcoplasmic and endoplasmic reticulum Ca 2C -ATPase (SERCA). A follow-up experiment in which gill cells were incubated with STC1 revealed a downregulation of the epithelial Ca 2C channel (ecacl) but an upregulation of cox-2 expression. The ECaCl is a gatekeeper for Ca 2C entry, whereas COX-2 mediates an activation of SERCA. Taking these results together, the present study is, to our knowledge, the first to provide evidence of STC1 binding and signaling as well as the first to decipher the mechanism of the effect of STC1 on fish gills.

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Characterization of ion channel and transporter mRNA expressions in isolated gill chloride and pavement cells of seawater acclimating eels

Cited by 115 publications

References 26 publications

Isolation and fractionation of gill cells from freshwater (Lasmigona costata) and seawater (Mesodesma mactroides) bivalves for use in toxicological studies with copper

Isolation and fractionation of gill cells from freshwater (Lasmigona costata) and seawater (Mesodesma mactroides) bivalves for use in toxicological studies with copper

Effects of cortisol and salinity acclimation on Na<sup>+</sup>/K<sup>+</sup>/2Cl<sup>–</sup>- cotransporter gene expression and Na<sup>+</sup>, K<sup>+</sup>-ATPase activity in the gill of Persian sturgeon, <i>Acipenser persicus</i>, fry

Characterization of stanniocalcin 1 binding and signaling in gill cells of Japanese eels

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