Characterization of intraperitoneal cytokines and nitrites in women with severe ovarian hyperstimulation syndrome

Revel, Ariel; Barak, V.; Lavy, Yuval; Anteby, Eyal Y.; Abramov, Yoram; Schenker, Joseph; Amit, Ami; Finci-Yeheskel, Z; Mayer, Michael; Simon, Alex; Laufer, Neri; Hurwitz, Arye

doi:10.1016/s0015-0282(16)58389-7

Cited by 61 publications

(6 citation statements)

References 21 publications

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“…Our clinical data which showed that melatonin FF concentrations positively correlated with serum E2 levels measured on the day of hCG injection as well as the OPU day also supported this point. It was widely accepted that both high level of VEGF and low level of NO can increase the permeability of cell membrane, resulting in the occurrence of OHSS [34, 35]. Previous studies have also confirmed NO can inhibit the production of estrogen, and iNOS can regulate vascular immune inflammation [20, 36].…”

Section: Discussionmentioning

confidence: 99%

Higher melatonin in the follicle fluid and MT2 expression in the granulosa cells contribute to the OHSS occurrence

Fang

et al. 2019

Reprod Biol Endocrinol

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Background Ovarian hyperstimulation syndrome (OHSS) is a common and severe complication for patients undergoing IVF/ICSI-ET. Melatonin widely participates in the regulation of female reproductive endocrine activity. However, whether melatonin participates in the progression of OHSS is largely unknown. This study aims to identify the predictive value of follicular fluid (FF) melatonin for OHSS establishment and the underlying mechanism. Methods All participants of this case-control study were enrolled at the Reproductive Medicine Center located in the First Affiliated Hospital of Zhengzhou University in China from January to October in 2017. Quantitative real-time PCR and western blot were used to examine the mRNA and protein levels. Primary granulosa cells were extracted and cultured for in vitro studies. Melatonin concentration was measured by ELISA. Logistic analysis and receiver-operating characteristic (ROC) curves were used to evaluate the predicting value of melatonin on OHSS occurrence. Main outcome measures The expression level of melatonin receptor 2 (MT2), P450 aromatase cytochrome (aromatase), vascular endothelial growth factor (VEGF), and inducible nitric oxide synthase (iNOS) mRNA in human primary granulosa cells. The concentration of melatonin in FF. The predicting value of melatonin on OHSS and the cut-off value of the prediction. Results FF melatonin concentrations were significantly higher in patients with OHSS compared to non-OHSS group (35.94 ± 10.18 ng/mL vs 23.93 ± 10.94 ng/mL, p <0.001). The expression of MT2 mRNA ( p = 0.0459) and protein in granulosa cells was also significantly higher in the OHSS group. When using a cut-off level of 27.52 ng/ml, the sensitivity and specificity of FF melatonin to predict OHSS was 84.6 and 74.0%, respectively ( p < 0.0001). We also found that melatonin could up-regulates aromatase mRNA, VEGF mRNA expression and down-regulates iNOS mRNA expression in the granulosa cells . Conclusion OHSS patients have higher melatonin in the FF as well as higher MT2 expression in the granulosa cells. The melatonin in FF might be used as an effective predictor for the occurrence of OHSS.

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Section: Discussionmentioning

confidence: 99%

Higher melatonin in the follicle fluid and MT2 expression in the granulosa cells contribute to the OHSS occurrence

Fang

et al. 2019

Reprod Biol Endocrinol

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“…IL-1 and IL-6 have been shown to induce changes in vascular permeability (12,36). It may be speculated that the elevated levels of these cytokines in endometriotic tissue may contribute to increased vascular permeability at the adhesion site, thus increasing the inflammatory reaction.…”

Section: Discussionmentioning

confidence: 99%

“…Dysmenorrhoea is referred to as a pronounced local hypertension in the uterine cavity due to a hypercontraction of the uterine muscle. This is probably stimulated by prostaglandins and has been shown to cause ischemia in the myometrium (36). IL-1␤ stimulates PGE2 production by endometrial epithelial cells (31), and a local release of IL-1␤ may contribute to this reaction.…”

Section: Discussionmentioning

confidence: 99%

Interleukin 1β, interleukin-6, and tumor necrosis factor-α in endometriotic tissue and in endometrium

Bergqvist

Bruse²,

Carlberg³

et al. 2001

Fertility and Sterility

166

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“…Several cytokines (TNF-␣, interleukin-1 and -6) that have been shown to play a role in the pathogenesis of increased vascular permeability are elevated in OHSS patients (122). Leptin is also linked to the cytokine family and a recent study investigated serum leptin concentrations in patients with severe OHSS during IVF-ET treatment (123).…”

Section: Mantzoros Et Al (mentioning

confidence: 99%

Interactions of the hormones leptin, ghrelin, adiponectin, resistin, and PYY3-36 with the reproductive system

Budak

Sánchez

Bellver

et al. 2006

Fertility and Sterility

200

152

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Characterization of intraperitoneal cytokines and nitrites in women with severe ovarian hyperstimulation syndrome

Cited by 61 publications

References 21 publications

Higher melatonin in the follicle fluid and MT2 expression in the granulosa cells contribute to the OHSS occurrence

Higher melatonin in the follicle fluid and MT2 expression in the granulosa cells contribute to the OHSS occurrence

Interleukin 1β, interleukin-6, and tumor necrosis factor-α in endometriotic tissue and in endometrium

Interactions of the hormones leptin, ghrelin, adiponectin, resistin, and PYY3-36 with the reproductive system

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