Characterization of interleukin 5 receptors on eosinophilic sublines from human promyelocytic leukemia (HL-60) cells.

Abstract

SummaryThe T cell product interleukin 5 (IL 5) has been shown to be a key factor in the development and the maturation of the eosinophilic cell lineage . We report here on the detection of human IL5 receptors on eosinophilic sublines of the promyelocytic leukemia HL-60. Sodium butyrate, which initiates differentiation to mature eosinophils, also induces the appearance of high affinity (Kd 1-5 x 10 -11 M) IL5 binding sites on these cells. The receptors are specific for 11,5, since binding of radiolabeled ligand can only be inhibited with homologous or murine IL-5 and not by other cytokines. We further show that the receptors are functional, since 116 can stimulate the proliferation of these cells. Affinity crosslinking of surface-bound 1251 human IL-5 or 35S mouse IL5 identified two membrane polypeptides of -60 and -130 kD to which 1175 is closely associated. The presence of granuloryte/macrophage-colony-stimulating factor or tumor necrosis factor during butyrate induction decreased the expression of 11,5 binding sites compared with control cultures. The identification and characterization of human 1175 receptors on HL60 sublines should provide new insight into the role of this cytokine in eosinophil differentiation .

IL-5 Up-Regulates Cysteinyl Leukotriene 1 Receptor Expression in HL-60 Cells Differentiated into Eosinophils

“…Butyric acid is thought to activate genes by blocking the actions of deacetylases, thereby inhibiting histone binding to DNA due to increased histone acetylation (50). This may lead to the observed induction of IL-5R␣ expression during eosinophil differentiation with butyric acid (42). Signaling through IL-5R␣/IL-5R␤ leads to activation of Janus kinase 2 and subsequent phosphorylation and dimerization of STAT factors.…”

“…When they are differentiated toward the eosinophil phenotype, HL-60 cells present several markers and characteristics of mature eosinophils (39,40). Furthermore, culture in the presence of butyric acid renders HL-60 cells responsive to IL-5, owing to induction of surface IL-5 receptor expression (42). Thus, HL-60/eos cells provide a useful model with which to study the modulation of CysLT 1 R. In the present study, we set out to determine whether the cytokine IL-5 could modulate the expression of CysLT 1 R on HL-60/eos cells.…”

Ligand binding of leukocyte integrin very late antigen‐4 involves exposure of sulfhydryl groups and is subject to redox modulation

“…The HL-60 clone 15 cells were treated with 0.5 mM butyric acid for 4 days to differentiate to an eosinophil-like phenotype [48]. The differentiated HL-60 clone 15 cells expressed VLA-4 and LFA-1 integrins on the cell surface at comparable levels (MFI: 19.47 and 19.06 for VLA-4 and LFA-1, respectively).…”

Hemopoietic Mechanisms in Allergic Airway Inflammation

“…Observations we have made regarding the induction of differentiation of Eo/B in leukemic cell lines demonstrate both IgER and IL–5R expression at relatively early stages of differentiation of this lineage [28, 29, 30]. …”