Characterization of intercellular junctions in the preimplantation mouse embryo by freeze-fracture and thin-section electron microscopy

Magnuson, Terry; Demsey, Anthony; Stackpole, Christopher W.

doi:10.1016/0012-1606(77)90296-2

Cited by 129 publications

(31 citation statements)

References 25 publications

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“…Compaction is accompanied by the first occurrence of epithelial structures with E-cadherin [Vestweber et al, 1987;Johnson and Ziomek, 1981] and tight junctions [Magnuson et al, 1977], and by the establishment of a basal-apical polarization of the outer cells [Lehtonen, 1980] which leads to the formation of the presumptive inner cell mass and an outer cell layer, the trophectoderm [Mü ntener and Hsu, 1977;Fleming, 1986]. At the time of compaction, an induction of direct intercellular communication is first detected in correlation with gap junctional plaques [Lo and Gilula, 1979;Lee et al, 1987].…”

Section: Introductionmentioning

confidence: 99%

Expression of connexin31 and connexin43 genes in early rat embryos

Reuss¹,

Hellmann²,

Traub³

et al. 1997

Dev. Genet.

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Section: Introductionmentioning

confidence: 99%

Expression of connexin31 and connexin43 genes in early rat embryos

Reuss¹,

Hellmann²,

Traub³

et al. 1997

Dev. Genet.

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“…GJs are also expressed during the early blastula stage (Dorresteijn et al, 1982;Magnuson et al, 1977), and persist throughout embryogenesis (Bennett et al, 1981). GJs significantly play an important role in preparing the uterus for embryo implantation as well as in the control of trophoblast invasion (Grummer et al, 1996).…”

Section: The Involvement Of Gap Junction Proteins In Embryogenesismentioning

confidence: 99%

Structure and function of gap junction proteins: role of gap junction proteins in embryonic heart development

Ahir¹,

Pratten²

2014

Int. J. Dev. Biol.

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Intercellular (cell-to-cell) communication is a crucial and complex mechanism during embryonic heart development. In the cardiovascular system, the beating of the heart is a dynamic and key regulatory process, which is functionally regulated by the coordinated spread of electrical activity through heart muscle cells. Heart tissues are composed of individual cells, each bearing specialized cell surface membrane structures called gap junctions that permit the intercellular exchange of ions and low molecular weight molecules. Gap junction channels are essential in normal heart function and they assist in the mediated spread of electrical impulses that stimulate synchronized contraction (via an electrical syncytium) of cardiac tissues. This present review describes the current knowledge of gap junction biology. In the first part, we summarise some relevant biochemical and physiological properties of gap junction proteins, including their structure and function. In the second part, we review the current evidence demonstrating the role of gap junction proteins in embryonic development with particular reference to those involved in embryonic heart development. Genetics and transgenic animal studies of gap junction protein function in embryonic heart development are considered and the alteration/disruption of gap junction intercellular communication which may lead to abnormal heart development is also discussed.

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“…Ultrastructural analysis of embryos indicates that tight junctions begin to form at compaction; however, this process is not completed until the late morula stage (16-to 32-cell stage), up to 24 hours later (Ducibella and Ander son, 1975;Magnuson et al, 1977;Pratt, 1985). Once completed, vectorial transport and blas tocoel accumulation can begin, dependent upon the segre gation of Na+,K+-ATPase to basolateral trophectoderm membranes (Watson and Kidder, 1988).…”

Section: Tight Junction Constructionmentioning

confidence: 99%

“…Desmosomes form relatively late in the process of trophectoderm differentiation, at the 32 cell stage once blastocoel formation has begun Magnuson et al, 1977;Jackson et al, 1980;. In contrast to the tight junction, the first desmosomes do not appear to form gradually, but rather display a mature morphology and molecular composition.…”

Section: Desmosome Formationmentioning

confidence: 99%

Biogenesis of structural intercellular junctions during cleavage in the mouse embryo

Fleming

Javed

Collins

et al. 1993

Journal of Cell Science

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SUMMARYThe preimplantation embryo differentiates the trophectoderm epithelium which, from the 32-cell stage, gener ates the blastocoel of the blastocyst and, after implan tation, gives rise to most extraembryonic lineages of the conceptus. Trophectoderm differentiation begins at compaction (8-cell stage) when cell-cell adhesion, medi ated by uvomorulin, and epithelial cell polarisation first occur. Here, we review our work on the biogenesis of tight junctions and desmosomes during epithelial differ entiation. Tight junction construction begins at com paction and appears to be a gradual process, both at morphological and molecular levels. This maturation pattern may be due in part to sequential expression of tight junction constituents from the embryonic genome. Tight junction formation is dependent upon uvomorulin adhesion but can be inhibited by different means with out apparently disturbing cell adhesion or polarisation. Cell interactions appear to regulate tight junction tissue specificity, in part by controlling the level of synthesis of constituents. Desmosome formation begins at the 32 cell stage, particularly as the embryo initiates blastocoel accumulation, and, in contrast with tight junction for mation, does not appear to be a gradual process. Thus, nascent desmosomes appear mature in terms of their molecular composition. Desmosomal proteins are syn thesised well in advance of desmosome formation but the synthesis of the principal glycoprotein components begins at the blastocyst stage and may regulate the timing of junction assembly. Implications of these dif fering patterns of biogenesis for the embryo are dis cussed.

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Characterization of intercellular junctions in the preimplantation mouse embryo by freeze-fracture and thin-section electron microscopy

Cited by 129 publications

References 25 publications

Expression of connexin31 and connexin43 genes in early rat embryos

Expression of connexin31 and connexin43 genes in early rat embryos

Structure and function of gap junction proteins: role of gap junction proteins in embryonic heart development

Biogenesis of structural intercellular junctions during cleavage in the mouse embryo

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