Characterization of inter and intra anastomosis group of Rhizoctonia spp. isolated from different crops in Peninsular Malaysia

Rashed, Osamah; Abdullah, Siti Nor Akmar; Alsultan, Wael; Misawa, Tomoo; Ahmad, Khalil; Kutawa, Abdulaziz Bashir

doi:10.1007/s40858-021-00433-5

Cited by 6 publications

(5 citation statements)

References 46 publications

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“…Other factors such as spontaneous natural mutations, genetic drift, gene flow, crop rotation, sampling year, and alternate host might have played significant role in terms of variation among the isolates of C. falcatum [70,[75][76][77][78]. Furthermore, molecular markers are more informative than rDNA-ITS sequencing in terms of detecting the diversity within the individual population but less successful in detecting the variation between different populations [79].…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic Analysis and Genetic Diversity of Colletotrichum falcatum Isolates Causing Sugarcane Red Rot Disease in Bangladesh

Hossain

Ahmad

Vadamalai

et al. 2021

Biology

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Colletotrichum falcatum Went causes red rot disease in sugarcane farming in the tropical and sub-tropical regions. This disease causes significant economic loss to the sugarcane production industry. Successful disease management strategies depend on understanding the evolutionary relationship between pathogens, genetic diversity, and population structure, particularly at the intra-specific level. Forty-one isolates of C. falcatum were collected from different sugarcane farms across Bangladesh for molecular identification, phylogeny and genetic diversity study. The four genes namely, ITS-rDNA, β-tubulin, Actin and GAPDH sequences were conducted. All the 41 C. falcatum isolates showed a 99–100% similarity index to the conserved gene sequences in the GenBank database. The phylogram of the four genes revealed that C. falcatum isolates of Bangladesh clustered in the same clade and no distinct geographical structuring were evident within the clade. The four gene sequences revealed that C. falcatum isolates from Bangladesh differed from other countries´ isolates because of nucleotides substitution at different loci. The genetic structure of C. falcatum isolates were determined using ISSR marker generated 404 polymorphic loci from 10 selected markers. The percentage of polymorphic loci was 99.01. The genetic variability at species level was slightly higher than at population level. Total mean gene diversity at the species level was 0.1732 whereas at population level it was 0.1521. The cluster analysis divided 41 isolates into four main genetic groups and the principal component analysis was consistent with cluster analysis. To the best of our knowledge, this is the first finding on characterizing C. falcatum isolates infesting sugarcane in Bangladesh. The results of this present study provide important baseline information vis a vis C. falcatum phylogeny analysis and genetic diversity study.

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Section: Discussionmentioning

confidence: 99%

Phylogenetic Analysis and Genetic Diversity of Colletotrichum falcatum Isolates Causing Sugarcane Red Rot Disease in Bangladesh

Hossain

Ahmad

Vadamalai

et al. 2021

Biology

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“…They stated that the criteria of the geographical area could be examined and mentioned the role of the anastomosis group in creating more critical diversity. Although other researchers have reported a genetic relationship and geographical location (25,26), its incompatibility can be found in other factors that cause genetic diversity. In this study, at the level of 80% similarity, the number of groups increased to 23 groups, which indicates the very high diversity observed among the isolates.…”

Section: Results Of Cluster Analysismentioning

confidence: 95%

Genetic diversity of rice sheath blight in different geographical regions of China

Li¹,

Wang²,

Li³

et al. 2022

Cell Mol Biol (Noisy-le-grand)

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“…botrytis L.), spinach (Spinacea oleracea L.), radish (Raphanus sativus L.) and cabbage (Brassica oleracea L. var. capitata L.) in Malaysia (Rashed et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Thus, studies of cross‐pathogenicity of binucleate Rhizoctonia AG‐Fb are needed to identify species and genotypes susceptible to the phytopathogen. For example, an isolate of binucleate Rhizoctonia obtained from spinach was pathogenic to cauliflower, radish and cabbage in Malaysia (Rashed et al., 2021). Additionally, it was observed that isolates of binucleate Rhizoctonia from native pastures also exhibited pathogenicity towards soybean crops (Kodati et al., 2021).…”

Section: Discussionmentioning

confidence: 99%

Characterization and report of binucleate Rhizoctonia AG‐Fb affecting soybean in Brazil

Cappellaro,

Huzar‐Novakowiski,

Chiomento

et al. 2024

Journal of Phytopathology

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Rhizoctonia root rot is a disease that can affect soybean leading to plant death usually in bare patches. Frequently, Rhizoctonia solani has been reported as the main causal agent of the disease. However, due to the great genetic diversity of the genus Rhizoctonia, there are other species that could also attack soybean plants. Thus, the objective of this study was to determine the pathogenicity and characterize an isolate of binucleate Rhizoctonia obtained from a soybean plant with symptoms of root rot cultivated in soil from southern Brazil. The isolate was identified based on the morphology and phylogenetic analysis of the internal transcribed spacer region (ITS) of the rDNA. Pathogenicity tests were then performed on seeds and roots of soybean and the optimum temperature for mycelial growth was determined. The isolate was identified as binucleate Rhizoctonia AG‐Fb and it was pathogenic to soybean seeds and roots. In the pathogenicity assay in soybean seed, the isolate reduced seed germination by 20% and caused seed rot index of 69.3. In the pathogenicity assay in soybean root, the inoculation of binucleate Rhizoctonia AG‐Fb caused 67.3 root rot severity index, and reduced the emergence speed index by 19%, seedling height by 31%, root dry mass by 25% and root volume by 43% in soybean seedlings. The optimum temperature for the mycelial growth of the isolate was 26°C. To the best of our knowledge, this is the first report of binucleate Rhizoctonia AG‐Fb associated with soybean root rot in Brazil.

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Characterization of inter and intra anastomosis group of Rhizoctonia spp. isolated from different crops in Peninsular Malaysia

Cited by 6 publications

References 46 publications

Phylogenetic Analysis and Genetic Diversity of Colletotrichum falcatum Isolates Causing Sugarcane Red Rot Disease in Bangladesh

Phylogenetic Analysis and Genetic Diversity of Colletotrichum falcatum Isolates Causing Sugarcane Red Rot Disease in Bangladesh

Genetic diversity of rice sheath blight in different geographical regions of China

Characterization and report of binucleate Rhizoctonia AG‐Fb affecting soybean in Brazil

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