Characterization of integrons in multiple antimicrobial resistant Escherichia coli isolates from bovine endometritis

Zhao, Hongxia; Shen, Jianzhong; An, Xiaoping; Fan, Hong-Liang; Cao, Jinshan; Li, Peifeng

doi:10.1016/j.rvsc.2010.09.004

Cited by 13 publications

(10 citation statements)

References 6 publications

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“…Furthermore, the pooled prevalence of class 1 integrons was calculated as 33%. The prevalence of class 1 integrons in E. coli isolates has been variable in different reports ranging from > 50% in studies conducted in Chile (37.3% in Poultry), Korea (31.7% in poultry and Swine), China (41.94% in chicken farm), China (36.8% in bovine), and Norwegian origin (12% in swine and poultry meat), being partially consistent with our findings [15,[32][33][34][35]. Compared to our study, Su et al investigated the occurrence of integrons in Enterobacteriaceae isolated from integrated fish farms.…”

Section: Discussionsupporting

confidence: 91%

“…Additionally, newly extended spectrum beta lactamases (ESBL)-encoding genes are usually located on integronlike structures (such as blaCTX-M, blaGES, or blaVEB-1) [14]. In this regard, since antibiotics are widely used in animal sources, the presence of integrons and antibiotic resistant gene in animal products has become a widespread concern [15].…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of class 1 integron in Escherichia coli isolated from animal sources in Iran: a systematic review and meta-analysis

2020

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Background: Among the genetic elements, integrons may contribute to the widespread incidence and spreading of antibiotic resistance among Escherichia coli isolates. Accordingly, this review aims to investigate the prevalence of class 1 integron in E. coli isolated from animal sources in Iran. Methods: This systematic literature search was performed from January 1, 2000 to the end of May 1, 2019. Then, publications that met our inclusion criteria were selected for data extraction and analysis. Also, the quality of included studies was independently assessed by two researchers based on the Joanna Briggs Institute. Metaanalysis was performed by the Comprehensive Meta-Analysis (CMA) software using the random effects model, Cochran's Q, and I 2 tests. Publication bias was estimated by funnel plot and Egger's linear regression test. Results: Based on inclusion criteria, five studies were included to meta-analysis. From those studies, the pooled prevalence of integrons was 33% (95% CI, 23.8-43.7%) ranging from 23.8 to 52.4%. There was a significant heterogeneity among the 5 studies (χ 2 = 11.73; p < 0.019; I 2 = 65.91%). Additionally, Begg's and Egger's tests were performed to quantitatively evaluate the publication biases. According to the results of Begg's test (z = 1.22, p = 0.22) and Egger's test (t = 3.03, p = 0.056), a significant publication bias was not observed. Conclusions: Our finding revealed the relatively high prevalence of class 1 integrons among E. coli isolates. Moreover, there was a significant heterogeneity among studies and subgroup analysis also showed that there was no difference about prevalence of class 1 integrons among different sample source.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Prevalence of class 1 integron in Escherichia coli isolated from animal sources in Iran: a systematic review and meta-analysis

2020

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“…The occurrences of class 1 integrons in the E. coli population tested are close to the results generally reported in WWTP effluents or in aquatic environments contaminated by these effluents (Ferreira da Silva et al ., ; Laroche et al ., ). Most of these integrons were detected in the multiresistant subpopulation, which is consistent with many studies that have shown the correlation between the carriage of integrons and the multiresistance of isolates (Zhang et al ., ; Zhao et al ., ). Nevertheless, in the variable region of these integrons, we found dfrA1 and aadA1 gene cassettes, which explain only the phenotypes of resistance to streptomycin and trimethoprim.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic-resistant Escherichia coli in karstic systems: a biological indicator of the origin of fecal contamination?

Ribeiro

Laroche²,

Hanin³

et al. 2012

FEMS Microbiol Ecol

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Occurrences of antibiotic‐resistant Escherichia coli in two springs of a karstic system (NW France) providing drinking water were determined to study the role of aquifers in the dissemination of the resistance genes. Water samples were collected during wet and dry periods and after a heavy rainfall event to investigate E. coli density, antibiotic resistance patterns, and occurrences of class 1, 2, and 3 integrons. By observing patterns of the resistant isolates (i.e. number and type of resistances) and their occurrences, we were able to define two resistant subpopulations, introduced in the aquifer via surface water: (1) R1‐2, characterized by one or two resistance(s), essentially to chloramphenicol and/or tetracycline (96.5%), was always found during the heavy rainfall event; (2) R3‐10, characterized by three or more resistances, mostly resistant to tetracycline (94.1%) and beta‐lactams (86%), was found transiently. Class 1 and 2 integrons were detected, mostly in the R3‐10 subpopulation for class 1 integrons. The characteristics of these two subpopulations strongly suggest that the contamination originates from pasture runoff for the R1‐2 subpopulation and from wastewater treatment plant effluents for the R3‐10 subpopulation. These two subpopulations of E. coli could be used as biological indicators to determine the origin of groundwater contamination.

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“…In the present study, we reported the frequency of MDR and the incidence of class 1 integrons from nonclinical swine and were 69.7 and 74.6% in commensal E. coli , which was lower than those that had been reported among swine origin nonclinical E. coli in several provinces of China, such as Sichuan (99.23, 87.69%; Lin et al, 2014 ), Xiamen (100, 92.2%; Liu et al, 2015 ), Harbin (100, 91.67%; Zhang et al, 2013 ), Hulunbeir (77.8, 95.59%; Qiu, 2015 ), Liaoning (92.31, 71.43%; Zhao et al, 2017 ), and Yunnan (96.15, 84.62%; Li et al, 2019 ) between 2010 and 2019. This indicates that there is a clear difference in prevalence between the previous investigated districts and the four provinces.…”

Section: Discussionmentioning

confidence: 71%

Diverse Gene Cassette Arrays Prevail in Commensal Escherichia coli From Intensive Farming Swine in Four Provinces of China

Zhang

Wang

et al. 2020

Front. Microbiol.

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Multiple-drug resistance bacteria containing antimicrobial resistance genes (ARGs) are a concern for public health. Integrons are bacterial genetic elements that can capture, rearrange, and express mobile gene cassettes responsible for the spread of ARGs. Few studies link genotype and phenotype of swine-related ARGs in the context of mobile gene cassette arrays among commensal Escherichia coli ( E. coli ) in nonclinical livestock isolates from intensive farms. In the present study, a total of 264 isolates were obtained from 330 rectal swabs to determine the prevalence and characteristics of antibiotic-resistant gene being carried by commensal E. coli in the healthy swine from four intensive farms at Anhui, Hebei, Shanxi, and Shaanxi, in China. Antimicrobial resistance phenotypes of the recovered isolates were determined for 19 antimicrobials. The E. coli isolates were commonly nonsusceptible to doxycycline (75.8%), tetracycline (73.5%), sulfamethoxazole-trimethoprim (71.6%), amoxicillin (68.2%), sulfasalazine (67.1%), ampicillin (58.0%), florfenicol (56.1%), and streptomycin (53.0%), but all isolates were susceptible to imipenem (100%). Isolates [184 (69.7%)] exhibited multiple drug resistance with 11 patterns. Moreover, 197 isolates (74.6%) were detected carrying the integron-integrase gene ( intI 1) of class 1 integrons. A higher incidence of antimicrobial resistance was observed in the intI 1-positive E. coli isolates than in the intI 1-negative E. coli isolates. Furthermore, there were 17 kinds of gene cassette arrays in the 70 integrons as detected by sequencing amplicons of variable regions, with 66 isolates (94.3%) expressing their gene cassettes encoding for multiple drug resistance phenotypes for streptomycin, neomycin, gentamicin, kanamycin, amikacin, sulfamethoxazole-trimethoprim, sulfasalazine, and florfenicol. Notably, due to harboring multiple, hybrid, and recombination cassettes, complex cassette arrays were attributed to multiple drug resistance patterns than simple arrays. In conclusion, we demonstrated that the prevalence of multiple drug resistance and the incidence of class 1 integrons were 69.7 and 74.6% in commensal E. coli isolated from healthy swine, which were lower in frequency than that previously reported in China.

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Characterization of integrons in multiple antimicrobial resistant Escherichia coli isolates from bovine endometritis

Cited by 13 publications

References 6 publications

Prevalence of class 1 integron in Escherichia coli isolated from animal sources in Iran: a systematic review and meta-analysis

Prevalence of class 1 integron in Escherichia coli isolated from animal sources in Iran: a systematic review and meta-analysis

Antibiotic-resistant Escherichia coli in karstic systems: a biological indicator of the origin of fecal contamination?

Diverse Gene Cassette Arrays Prevail in Commensal Escherichia coli From Intensive Farming Swine in Four Provinces of China

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