Characterization of Infectious Bursal Disease Viruses from Argentina

Remorini, Patricia; Calderón, Marina Gallo; Aguirre, Sebastián; Periolo, Osvaldo; Torre, J.L. La; Mattion, Nora

doi:10.1637/7447-092605r.1

Cited by 8 publications

(7 citation statements)

References 25 publications

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“…Accordingly, the genetic analysis cannot accurately assign this group of viruses, here denoted as dIBDVs, to the typical c, ca, va and vv strains. These Uruguayan and Argentine dIBDVs are included in an independent evolutionary lineage with previously reported viruses from other countries in America (Jackwood & Sommer, 1997;Ikuta et al, 2001;Banda et al, 2003;Remorini et al, 2006;Jackwood & Sommer-Wagner, 2007;Ojkic et al, 2007), Europe (Domanska et al, 2004) and Asia (Kwon et al, 2000) (Figure 2). The hvVP2 Figure 1.…”

Section: Discussionsupporting

confidence: 74%

“…Isolated IBDVs with different traits than the traditional strains have also been sporadically reported through the years in different parts of the world (Ikuta et al, 2001;Domanska et al, 2004;Remorini et al, 2006;Jackwood & Sommer-Wagner, 2007). These IBDVs have been generally considered atypical isolates or variants that have evolved in restricted geographic regions or during short periods of time under particular conditions.…”

Section: Introductionmentioning

confidence: 99%

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Genetic characterization of South American infectious bursal disease virus reveals the existence of a distinct worldwide-spread genetic lineage

et al. 2015

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Infectious bursal disease virus (IBDV) is one of the most concerning health problems for world poultry production. IBDVs comprise four well-defined evolutionary lineages known as classic (c), classic attenuated (ca), variant (va) and very virulent (vv) strains. Here, we characterized IBDVs from South America by the genetic analysis of both segments of the viral genome. Viruses belonging to c, ca and vv strains were unambiguously classified by the presence of molecular markers and phylogenetic analysis of the hypervariable region of the vp2 gene. Notably, the majority of the characterized viruses (9 out of 15) could not be accurately assigned to any of the previously described strains and were then denoted as distinct (d) IBDVs. These dIBDVs constitute an independent evolutionary lineage that also comprises field IBDVs from America, Europe and Asia. The hypervariable VP2 sequence of dIBDVs has a unique and conserved molecular signature (272T, 289P, 290I and 296F) that is a diagnostic character for classification. A discriminant analysis of principal components (DAPC) also identified the dIBDVs as a cluster of genetically related viruses separated from the typical strains. DAPC and genetic distance estimation indicated that the dIBDVs are one of the most genetically divergent IBDV lineages. The vp1 gene of the dIBDVs has non-vvIBDV markers and unique nucleotide and amino acid features that support their divergence in both genomic segments. The present study suggests that the dIBDVs comprise a neglected, highly divergent lineage that has been circulating in world poultry production since the early time of IBDV emergence.

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Section: Discussionsupporting

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Genetic characterization of South American infectious bursal disease virus reveals the existence of a distinct worldwide-spread genetic lineage

et al. 2015

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“…Those residues were present in four sequences reported in a previous study on Argentinean IBDV isolates [47]. In that report, the samples S002 (GenBank accession number AM084688), S003 (accession number AM084689), B641A34 (accession number AM084695), and P30903 (accession number AM084694) contained the residues characteristic of the Argentinean lineage.…”

Section: Discussionmentioning

confidence: 75%

Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage

Vera

Craig²,

Olivera³

et al. 2015

Arch Virol

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In Argentina, classical vaccines are used to control infectious bursal disease virus (IBDV); however, outbreaks of IBDV are frequently observed. This could be due to failures in the vaccination programs or to the emergence of new strains, which would be able to break through the protection given by vaccines. Hence, genetic characterization of the viruses responsible for the outbreaks that occurred in recent years is crucial for the evaluation of the control programs and the understanding of the epidemiology and evolution of IBDV. In this study, we characterized 51 field samples collected in Argentina (previously identified as IBDV positive) through the analysis of previously identified apomorphic sequences. Phylogenetic analysis of regVP2 showed that 42 samples formed a unique cluster (Argentinean lineage), seven samples were typical classical strains (one of them was a vaccine strain), and two belonged to the very virulent lineage (vvIBDV). Interestingly, when the analysis was performed on the regVP1 sequences, the field samples segregated similarly to regVP2; thus, we observed no evidence of a reassortment event in the Argentinean samples. Amino acid sequence analysis of regVP2 showed a particular pattern of residues in the Argentinean lineage, particularly the presence of T272, P289 and F296, which had not been reported before as signature sequences for any IBDV phenotype. Notably, the residue S254, characteristic of the antigenic variant, was not present in any of the Argentinean samples.

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“…By analysing the hvVP2 sequences available in the GenBank, we inferred that more than 10% of the IBDV sequences correspond to dIBDVs, suggesting a high frequency of this lineage in the global virus population. Countries such as Argentina, Canada and Uruguay have reported a high prevalence of this lineage circulating in the poultry production, while in countries such as Brazil, Colombia, Hungary, Poland, Puerto Rico, Russia, South Korea and the United States, there are only sporadic reports of dIBDVs (Shcherbakova et al, 1998;Kwon et al, 2000;Ikuta et al, 2001;Jackwood et al, 2001;Smiley & Jackwood, 2001;Domanska et al, 2004;Remorini et al, 2006;Jackwood & Sommer-Wagner, 2007;Ojkic et al, 2007;Hernández et al, 2015;Tomás et al, 2015;Vera et al, 2015). This uneven prevalence among different countries needs to be confirmed by performing more extensive studies with a specific diagnostic method, taking into consideration that dIBDVs can be easily ignored during routine surveillance due to the apparent lack of differential clinical signs (Ikuta et al, 2001;Domanska et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…This lineage is resolved in a well-supported clade and has a unique four-aminoacid signature (T272, P289, I290, F296). Most isolates that are now classified as dIBDV were initially considered atypical classic or variant strains that harboured unique nucleotide and amino acid changes as a consequence of local differentiation (Kwon et al, 2000;Ikuta et al, 2001;Domanska et al, 2004;Remorini et al, 2006;Jackwood & Sommer-Wagner, 2007;Ojkic et al, 2007). Some dIBDV isolates have exhibited mild clinical signs and antigenic differences (Ikuta et al, 2001;Domanska et al, 2004;Vera et al, 2015) but further phenotypic studies are needed to understand the epidemiological and sanitary relevance of this lineage that contains part of the genetic variability of the virus (Hernández et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Development of an RT-qPCR assay for the specific detection of a distinct genetic lineage of the infectious bursal disease virus

et al. 2016

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The infectious bursal disease virus (IBDV) is a major health threat to the world's poultry industry despite intensive controls including proper biosafety practices and vaccination. IBDV (Avibirnavirus, Birnaviridae) is a non-enveloped virus with a bisegmented double-stranded RNA genome. The virus is traditionally classified into classic, variant and very virulent strains, each with different epidemiological relevance and clinical implications. Recently, a novel worldwide spread genetic lineage was described and denoted as distinct (d) IBDV. Here, we report the development and validation of a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay for the specific detection of dIBDVs in the global poultry industry. The assay employs a TaqMan-MGB probe that hybridizes with a unique molecular signature of dIBDV. The assay successfully detected all the assessed strains belonging to the dIBDV genetic lineage, showing high specificity and absence of cross-reactivity with non-dIBDVs, IBDV-negative samples and other common avian viruses. Using serial dilutions of in vitro-transcribed RNA we obtained acceptable PCR efficiencies and determination coefficients, and relatively small intra- and inter-assay variability. The assay demonstrated a wide dynamic range between 10 and 10 RNA copies/reaction. This rapid, specific and quantitative assay is expected to improve IBDV surveillance and control worldwide and to increase our understanding of the molecular epidemiology of this economically detrimental poultry pathogen.

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Characterization of Infectious Bursal Disease Viruses from Argentina

Cited by 8 publications

References 25 publications

Genetic characterization of South American infectious bursal disease virus reveals the existence of a distinct worldwide-spread genetic lineage

Genetic characterization of South American infectious bursal disease virus reveals the existence of a distinct worldwide-spread genetic lineage

Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage

Development of an RT-qPCR assay for the specific detection of a distinct genetic lineage of the infectious bursal disease virus

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