Characterization of IgM molecules in light-chain deficient variants of a B-cell tumor

Taya, M; Rabinovich, Efrat; Haimovich, Joseph

doi:10.1016/0165-2478(92)90044-o

Cited by 4 publications

(4 citation statements)

References 22 publications

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“…Substantial amounts of and chains are present on the 38C-13 cells as well as on several Id-negative variants. However, as previously reported (24), no chains could be detected in certain variants. Nevertheless, chains are expressed on the cell surface of these -negative cells, albeit at much lower levels than on the parental 38C-13 and -positive, Id-negative variants.…”

Section: Isolation and Characterization Of Id-negative Variants Of Thmentioning

confidence: 59%

“…Nevertheless, chains are expressed on the cell surface of these -negative cells, albeit at much lower levels than on the parental 38C-13 and -positive, Id-negative variants. The -containing molecules on the -negative variants have previously been shown to consist of chains assembled with the 5 and VpreB polypeptide chains (24).…”

Section: Isolation and Characterization Of Id-negative Variants Of Thmentioning

confidence: 99%

“…The parental 38C-13 cell line is a ϩ , ␦ Ϫ , SLC-expressing murine B cell (21,24). The DB2 and EH Id-negative variant cell lines have previously been obtained in anti-Id-treated 38C-13 tumorbearing mice (25).…”

Section: Isolation and Characterization Of Id-negative Variants Of Thmentioning

confidence: 99%

“…Given the controversy surrounding the nature of the B cells undergoing secondary V(D)J recombination and its regulation, we have analyzed the murine 38C-13 B cell line (21), which has previously been shown to undergo light chain gene replacement (22,23). The 38C-13 cells are surface IgM ϩ , IgD Ϫ and produce both and surrogate light chains (24). During studies on Id-specific immunotherapy of 38C-13 tumors, it has been observed that Id-negative variant tumors develop in surviving mice (22,25).…”

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confidence: 99%

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Secondary V(D)J Rearrangements and B Cell Receptor-Mediated Down-Regulation of Recombination Activating Gene-2 Expression in a Murine B Cell Line

Maës

Caspi

Rougeon

et al. 2000

The Journal of Immunology

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It has recently become clear that recombination of Ig genes is not restricted to B cell precursors but that secondary rearrangements can also occur under certain conditions in phenotypically immature bone marrow and peripheral B cells. However, the nature of these cells and the regulation of secondary V(D)J recombination in response to B cell receptor (BCR) stimulation remain controversial. In the present study, we have analyzed secondary light chain gene rearrangements and recombination activating gene (RAG) expression in the surface IgM+, IgD− murine B cell line, 38C-13, which has previously been found to undergo κ light chain replacement. We find that 38C-13 cells undergo spontaneous secondary Vκ-Jκ and RS rearrangements in culture, with recombination occurring on both productive and nonproductive alleles. Both 38C-13 cells and the Id-negative variants express the RAG genes, indicating that the presence of RAG does not depend on activation via the 38C-13 BCR. Moreover, BCR cross-linking in 38C-13 cells leads to a rapid and reversible down-regulation of RAG2 mRNA. Therefore, 38C-13 cells resemble peripheral IgM+, IgD− B cells undergoing light chain gene rearrangement and provide a possible in vitro model for studying peripheral V(D)J recombination.

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Section: Isolation and Characterization Of Id-negative Variants Of Thmentioning

confidence: 59%

Section: Isolation and Characterization Of Id-negative Variants Of Thmentioning

confidence: 99%

Section: Isolation and Characterization Of Id-negative Variants Of Thmentioning

confidence: 99%

mentioning

confidence: 99%

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Secondary V(D)J Rearrangements and B Cell Receptor-Mediated Down-Regulation of Recombination Activating Gene-2 Expression in a Murine B Cell Line

Maës

Caspi

Rougeon

et al. 2000

The Journal of Immunology

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Light Chain Loss and Reexpression Leads to Idiotype Switch. Surrogate Light Chains are Probably Responsible for this Process

Caspi

Taya

Hollander

et al. 1995

Current Topics in Microbiology and Immunology

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Different assembly species of IgM are directed to distinct degradation sites along the secretory pathway.

Rabinovich

Bar-Nun

Amitay

et al. 1993

Journal of Biological Chemistry

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Characterization of IgM molecules in light-chain deficient variants of a B-cell tumor

Cited by 4 publications

References 22 publications

Secondary V(D)J Rearrangements and B Cell Receptor-Mediated Down-Regulation of Recombination Activating Gene-2 Expression in a Murine B Cell Line

Secondary V(D)J Rearrangements and B Cell Receptor-Mediated Down-Regulation of Recombination Activating Gene-2 Expression in a Murine B Cell Line

Light Chain Loss and Reexpression Leads to Idiotype Switch. Surrogate Light Chains are Probably Responsible for this Process

Different assembly species of IgM are directed to distinct degradation sites along the secretory pathway.

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