Characterization of<i>Streptomyces</i>sp. LS462 with high productivity of echinomycin, a potent antituberculosis and synergistic antifungal antibiotic

Chen, Caixia; Chen, Xiangyin; Ren, Biao; Guo, Hui; Abdel‐Mageed, Wael M.; Liu, Xueting; Song, Fuhang; Zhang, Lixin

doi:10.1093/jimb/kuab079

Cited by 9 publications

(9 citation statements)

References 57 publications

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“…Echinomycin bound to DNA has been shown to inhibit transcription in bacteria and chromatin condensation and DNA replication in eukaryotes, ultimately leading to cell cycle arrest (Fok & Waring, 1972; Krasinska & Fisher, 2009; May et al, 2004; Sato et al, 1967; Ward et al, 1965; Waring & Makoff, 1974). Quinomycins have been shown to possess antimicrobial, antiviral, and antitumor activities (Carter et al, 1954; Chen et al, 2021; Dolma et al, 2003; Sato et al, 1969; Yoshida et al, 1961).…”

Section: Introductionmentioning

confidence: 99%

The UvrA‐like protein Ecm16 requires ATPase activity to render resistance against echinomycin

Mera

2022

Molecular Microbiology

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Bacteria use various strategies to become antibiotic resistant. The molecular details of these strategies are not fully understood. We can increase our understanding by investigating the same strategies found in antibiotic‐producing bacteria. In this work, we characterize the self‐resistance protein Ecm16 encoded by echinomycin‐producing bacteria. Ecm16 is a structural homolog of the nucleotide excision repair protein UvrA. Expression of ecm16 in the heterologous system Escherichia coli was sufficient to render resistance against echinomycin. Ecm16 binds DNA (double‐stranded and single‐stranded) using a nucleotide‐independent binding mode. Ecm16’s binding affinity for DNA increased by 1.7‐fold when the DNA is intercalated with echinomycin. Ecm16 can render resistance against echinomycin toxicity independently of the nucleotide excision repair system. Similar to UvrA, Ecm16 has ATPase activity, and this activity is essential for Ecm16’s ability to render echinomycin resistance. Notably, UvrA and Ecm16 were unable to complement each other's function. Together, our findings identify new mechanistic details of how a refurbished DNA repair protein Ecm16 can specifically render resistance to the DNA intercalator echinomycin.

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Section: Introductionmentioning

confidence: 99%

The UvrA‐like protein Ecm16 requires ATPase activity to render resistance against echinomycin

Mera

2022

Molecular Microbiology

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“…Therefore, microbial pigments are preferred over their chemical counterparts. Added advantages are microbial pigment's ease of production and processing supplemented with diversified biological activities, such as antimicrobial, anticancer, antioxidant, and antituberculosis (Chatragadda & Dufossé, 2021 ; Chen et al., 2021 ; Silva et al., 2021 ). Different bacterial pigments with potential bioactivities have been summarized elsewhere (Venil et al., 2020 ; Celedón and Díaz, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Microbial pigments: Learning from Himalayan perspective to industrial applications

Kumar

Ambika

et al. 2022

Journal of Industrial Microbiology and Biotechnology

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Pigments are an essential part of life on earth, ranging from microbes to plants and humans. The physiological and environmental cues induce microbes to produce a broad spectrum of pigments, giving them adaptation and survival advantages. Microbial pigments are of great interest due to their natural origin, diverse biological activities, and wide applications in the food, pharmaceutical, cosmetics, and textile industries. Despite noticeable research on pigment-producing microbes, commercial successes are scarce, primarily from higher, remote, and inaccessible Himalayan niches. Therefore, substantial bioprospection integrated with advanced biotechnological strategies is required to commercialize microbial pigments successfully. The current review elaborates on pigment-producing microbes from a Himalayan perspective, offering tremendous opportunities for industrial applications. Additionally, it illustrates the ecological significance of microbial pigments and emphasizes the current status and prospects of microbial pigments production above the test tube scale.

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“…The results showed that the Streptomyces strains that produced the anti-Candida derivatives came from the most diverse environments. These included 16 studies collecting strains from distinct soil sediment samples [43][44][45][46][47][48][49][50][51][52][53][54][55][56][57][58], 11 studies obtaining strains from lakes, seas and oceans [59][60][61][62][63][64][65][66][67][68][69][70], and 5 studies isolating them from marine sponges. Additionally, one study derived strains from marine corals [71], while insects such as wasps, ants and cockroaches contributed isolates in five studies [72][73][74][75][76].…”

Section: Streptomyces-derived Compounds With Anti-candida Activity De...mentioning

confidence: 99%

Streptomyces: a natural source of anti-Candida agents

Martins,

Rodrigues da Silva,

Ratcliffe

et al. 2023

Journal of Medical Microbiology

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Introduction. There is an urgent need to source new compounds that can combat the current threat of serious infection caused by Candida spp. and contend with the problem of antimicrobial resistance. Gap. A synthesis of the evidence available from the current literature is needed to identify promising antifungal chemotherapeutics. Aim. To highlight anti-Candida compounds derived from Streptomyces spp. (a well-known source of antimicrobial compounds) that could translate to potential candidates for future clinical practice. Methodology. A comprehensive review was conducted across three scientific literature databases spanning a 13-year period. Results. We identified 151 compounds with anti-Candida activity. Amongst these, 40 were reported with very strong inhibitory activity, having minimum inhibitory concentrations (MICs) against Candida spp. of <3.5 µg ml−1, 66 compounds were considered strong inhibitors and 45 compounds exhibited moderate inhibitory potential. From an analysis of the MICs, we deduced that the actinomycin-like compounds RSP01 and RSP02 were probably the most promising anti-Candida compounds. Other antifungals of note included filipin-like compounds, which demonstrated superior inhibition to amphotericin B and activity against Candida glabrata and Candida krusei, and bafilomycin derivatives, which had substantial inhibition against Candida parapsilosis. Conclusion. It is essential to recognize the limitations inherent in the quest for new antifungals, which encompass toxicity, in vivo effectiveness and constraints associated with limited data access. However, further investigation through in-depth study and emerging technologies is of paramount importance, given that there are still many more compounds to discover. This review highlights the importance of antifungal compounds derived from Streptomyces , which demonstrate robust inhibition, and, in many cases, low toxicity, making them promising candidates for the development of novel antifungal agents.

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Characterization ofStreptomycessp. LS462 with high productivity of echinomycin, a potent antituberculosis and synergistic antifungal antibiotic

Cited by 9 publications

References 57 publications

The UvrA‐like protein Ecm16 requires ATPase activity to render resistance against echinomycin

The UvrA‐like protein Ecm16 requires ATPase activity to render resistance against echinomycin

Microbial pigments: Learning from Himalayan perspective to industrial applications

Streptomyces: a natural source of anti-Candida agents

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