Characterization of Staphylococcus aureus RecX protein: Molecular insights into negative regulation of RecA protein and implications in HR processes

Abstract

Homologous recombination is essential for genome stability and for maintaining genetic diversity. In eubacteria, RecA protein plays a key role during DNA repair, transcription, and homologous recombination. RecA is regulated at multiple levels, but majorly by RecX protein. Moreover, studies have shown RecX is a potent inhibitor of RecA, and thus acts as an antirecombinase. Staphylococcus aureus is a major food-borne pathogen that causes skin, bone joint, and bloodstream infections. To date RecX’s role in S. aureus has remained enigmatic. Here, we show that S. aureus RecX (SaRecX) is expressed during exposure to DNA-damaging agents and purified RecX protein directly interacts physically with RecA protein. The SaRecX is competent to bind with single strand DNA preferentially and double strand DNA feebly. Significantly, SaRecX impedes the RecA-driven displacement loop and inhibits formation of the strand exchange. Notably, SaRecX also abrogates adenosine triphosphate (ATP) hydrolysis and abolishes the LexA coprotease activity. These findings highlight the role of the RecX protein as an antirecombinase during homologous recombination, and plays a pivotal role in regulation of RecA during the DNA transactions.

Rhein against Staphylococcus xylosus by interfering with respiratory metabolism and inducing oxidative stress

“…In S . aureus , recA deficiency significantly reduced antibiotic-induced resistance ( Kiran and Patil, 2023 ). It has been shown that lexA-deficient mutant strains have a significantly reduced ability to adhere and biofilm formation ( Gotoh et al, 2010 ).…”