Characterization of<i>Mesorhizobium loti</i><scp>L</scp>-Rhamnose Isomerase and Its Application to<scp>L</scp>-Talose Production

Takata, Goro; Uechi, Keiko; Taniguchi, Eriko; Kanbara, Yuka; Yoshihara, Akihide; Morimoto, Kenji; Izumori, Ken

doi:10.1271/bbb.110018

Cited by 28 publications

(17 citation statements)

References 24 publications

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“…The specific activities of the enzymes isolated from P. stutzeri, Caldicellulosiruptor saccharolyticus (Lin et al 2011), Thermotoga maritime (Park et al 2010), Thermoanaerobacterium saccharolyticum (Hung et al 2014), Bacillus pallidus (Poonperm et al 2007), M. loti (Takata et al 2011), and Bacillus subtilis (Park 2014) toward L-mannose were 81.6, 38, 15, 6, 4.52, 2.27, and 0.92 U mg −1 , respectively. In addition, L-fructose has also been produced from L-mannose with a conversion ratio of approximately 25 % by an enzyme reactor packed with cross-linked Streptomyces rubiginosus D-glucose isomerase crystals (Jokela et al 2002).…”

Section: Isomerization Between L-fructose and L-mannosementioning

confidence: 99%

“…Unfortunately, none of the reported isomerases shows a high activity toward L-hexoses. Still, some aldose isomerases have been used for Laldohexose production, including L-rhamnose isomerase (EC 5.3.1.14) (Bhuiyan et al 1997;Leang et al 2004a;Takata et al 2011), L-ribose isomerase (EC 5.3.1.B3) (Terami et al 2015), and D-arabinose isomerase (EC 5.3.1.3) (Leang et al 2003). Some aldose phosphate isomerases have also shown potential for the application of L-aldohexose production, including D-ribose-5-phosphate isomerase (EC 5.3.1.6) (Park et al 2010), D-glucose-6-phosphate isomerase (EC 5.3.1.9) ), D-mannose-6-phosphate isomerase (EC 5.3.1.8) , and D-galactose-6-phosphate isomerase (EC 5.3.1.26) (Park et al 2007).…”

Section: C-3 Epimerization Between L-ketohexosesmentioning

confidence: 99%

“…The L-rhamnose isomerase from Pseudomonas stutzeri also produces both L-talose and L-galactose from L-tagatose (Leang et al 2004b). However, the M. loti L-rhamnose isomerase only catalyzes one-step isomerization between L-tagatose and L-talose (Takata et al 2011).…”

Section: Isomerization Of L-sorbose To L-gulose and L-idosementioning

confidence: 99%

“…Production of L-talose from L-tagatose L-Talose production was performed by an M. loti L-rhamnose isomerase immobilized on Chitopearl beads BCW 2510, which converted 300 g L −1 of L-tagatose to 30 g L −1 of Ltalose without any byproducts after 12 h of reaction (Takata et al 2011). The P. stutzeri L-rhamnose isomerase immobilized on BCW 2603 beads was also used for L-talose production, and it produced L-talose from 200 g L −1 of Ltagatose, with a conversion ratio of 12 % (Bhuiyan et al 1999).…”

Section: Production Of L-tagatose From L-talosementioning

confidence: 99%

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Advances in the enzymatic production of l-hexoses

Chen

Zhang

et al. 2016

Appl Microbiol Biotechnol

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Rare sugars have recently drawn attention because of their potential applications and huge market demands in the food and pharmaceutical industries. All L-hexoses are considered rare sugars, as they rarely occur in nature and are thus very expensive. L-Hexoses are important components of biologically relevant compounds as well as being used as precursors for certain pharmaceutical drugs and thus play an important role in the pharmaceutical industry. Many general strategies have been established for the synthesis of L-hexoses; however, the only one used in the biotechnology industry is the Izumoring strategy. In hexose Izumoring, four entrances link the D- to L-enantiomers, ketose 3-epimerases catalyze the C-3 epimerization of L-ketohexoses, and aldose isomerases catalyze the specific bioconversion of L-ketohexoses and the corresponding L-aldohexoses. In this article, recent studies on the enzymatic production of various L-hexoses are reviewed based on the Izumoring strategy.

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Section: Isomerization Between L-fructose and L-mannosementioning

confidence: 99%

Section: C-3 Epimerization Between L-ketohexosesmentioning

confidence: 99%

Section: Isomerization Of L-sorbose To L-gulose and L-idosementioning

confidence: 99%

Section: Production Of L-tagatose From L-talosementioning

confidence: 99%

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Advances in the enzymatic production of l-hexoses

Chen

Zhang

et al. 2016

Appl Microbiol Biotechnol

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“…14) The same has been reported for the Lrhamnose isomerase of M. loti. 26) They are regarded as important enzymes for the production of various rare sugars by the Izumoring strategy. If one can control the expression of these two enzymes in M. loti cells, there is Table 1 a possibility that it can produce rare sugars from natural sugars by microbial fermentation alone.…”

Section: Determination Of the Substrate Specificity And Kinetic Parammentioning

confidence: 99%

Gene Cloning and Characterization ofL-Ribulose 3-epimerase fromMesorhizobium lotiand Its Application to Rare Sugar Production

Uechi

Takata

Fukai

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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A gene encoding L-ribulose 3-epimerase (L-RE) from Mesorhizobium loti, an important enzyme for rare sugar production by the Izumoring strategy, was cloned and overexpressed. The enzyme showed highest activity toward L-ribulose (230 U/mg) among keto-pentoses and keto-hexoses. This is the first report on a ketose 3-epimerase showing highest activity toward keto-pentose. The optimum enzyme reaction conditions for L-RE were determined to be sodium phosphate buffer (pH 8.0) at 60 °C. The enzyme showed of higher maximum reaction a rate (416 U/mg) and catalytic efficiency (43 M(-1) min(-1)) for L-ribulose than other known ketose 3-epimerases. It was able to produce L-xylulose efficiently from ribitol in two-step reactions. In the end, 7.2 g of L-xylulose was obtained from 20 g of ribitol via L-ribulose at a yield of 36%.

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Organic Synthesis with Isomerases

2022

Enzyme‐Based Organic Synthesis

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Characterization ofMesorhizobium lotiL-Rhamnose Isomerase and Its Application toL-Talose Production

Cited by 28 publications

References 24 publications

Advances in the enzymatic production of l-hexoses

Advances in the enzymatic production of l-hexoses

Gene Cloning and Characterization ofL-Ribulose 3-epimerase fromMesorhizobium lotiand Its Application to Rare Sugar Production

Organic Synthesis with Isomerases

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