Characterization ofLpSK6: ALycopersiconGroup Three GSK-3/SHAGGY-Like Protein Kinase

Abstract

Members of the glycogen synthase kinase 3/SHAGGY-like protein kinase (GSK) family in plants are important regulators of stress response, development, and brassinosteroid signaling, and are highly homologous to animal GSKs. Several of the plant kinases are preferentially expressed during pollen development. We report the characterization of LpSK6, a member of the GSK family from the genus Lycopersicon. LpSK6 shows high sequence homology to members of group III, which contain a putative mitochondrial targeting peptide. LpSK6 is expressed 3 to 5 fold higher in anthers than in roots and stems, and is the first GSK gene isolated from genus Lycopersicon.

A Sugar-Inducible Protein Kinase, VvSK1, Regulates Hexose Transport and Sugar Accumulation in Grapevine Cells

“…Indeed, VvSK1 shared highest similarities with GSK3s from Solanaceae proteins NSK91, NSK59, and LpSK6 (87%, 87%, 86.6%), PSK6 and PSK7 (87% and 84.3%), and Arabidopsis ASK2 and ASK8 (78% and 86%). These GSK3s, which belong to the same clade (group III), are preferentially expressed in flowers (Tichtinsky et al, 1998;Charrier et al, 2002;Wilson et al, 2004). Among these, ASK (AtSK3-2) was shown to be involved in the control of cell elongation in flower development and in response to light (Claisse et al, 2007).…”