Characterization of<i>LeMir</i>, a Root-Knot Nematode-Induced Gene in Tomato with an Encoded Product Secreted from the Root1

Brenner, Eric D.; Lambert, Kris N.; Kaloshian, Isgouhi; Williamson, Valerie M.

doi:10.1104/pp.118.1.237

Cited by 64 publications

(43 citation statements)

References 66 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Surprisingly, none of these TI-like genes corresponded to win3, the previously characterized poplar Kunitz TI ). The respective top BLASTX hits of PtdTI3, PtdTI4, and PtdTI5 were the wound-induced P. tremuloides PtTI3 (86% amino acid identity; AAK32691), a Theobroma cacao storage protein/TI (33%; GenBank accession P32765), and a Sesbania rostratanodule-expressed Kunitz TI (46%; AJ441323), respectively ( Table 2) (Brenner et al 1998). However, in the case of PtdTI5, only six of eight consensus residues are present (data not shown).…”

Section: Identification and Analysis Of Kunitz Ti-like Genesmentioning

confidence: 97%

Gene expression profiling of systemically wound-induced defenses in hybrid poplar

Christopher

Miranda

Major

et al. 2004

Planta

View full text Add to dashboard Cite

As part of an ongoing effort to identify genes involved in poplar defense responses, and to provide a resource for comparative analysis of woody and non-woody plant defense, we generated expressed sequence tags (ESTs) from a library constructed from systemically wounded leaves of hybrid poplar (Populus trichocarpa x P. deltoides). Partial sequences were obtained from the 5' ends of 928 individual cDNAs, which could be grouped into 565 non-overlapping sequences. Of these, 447 sequences were singletons, while the remainder fell into 118 clusters containing up to 17 partially overlapping ESTs. Approximately 81% of the EST sequences showed similarity to previously described sequences in public databases. Of these, the distribution of gene functions within the EST set indicated that approximately 11% of the ESTs encode proteins potentially involved in defense or secondary metabolism, while photosynthesis and primary metabolism accounted for 45% of the expressed genes. Two types of defense proteins, Kunitz trypsin inhibitors and chitinases, were found among the ten most abundant ESTs, indicating the significant impact of wounding on the leaf transcriptome and suggesting that these functions are important for hybrid poplar defense. In the course of this work, three new wound-inducible Kunitz trypsin inhibitor-like genes and two new chitinase-like genes were characterized. A suite of other systemically wound-induced genes were identified using northern and macroarray analysis, indicating diversity and multiplicity in the induced defense response. Overall, we demonstrate that defense-related genes of hybrid poplar have a variety of functions, and show remarkably diverse expression patterns upon wounding.

show abstract

Section: Identification and Analysis Of Kunitz Ti-like Genesmentioning

confidence: 97%

Gene expression profiling of systemically wound-induced defenses in hybrid poplar

Christopher

Miranda

Major

et al. 2004

Planta

View full text Add to dashboard Cite

show abstract

“…Hypersensitive reaction (HR) is one of the most efficient forms of plant defense against pathogens (Karrer et al, 1998). LeMir (spots 16 and 17) was secreted and concentrated in roots in response to infestation by nematodes, suggesting its involvement in plant defense against these organisms (Brenner et al, 1998). Moreover, overexpression of this protein was observed in lesions caused by hypersensitive response in tomato plants, demonstrating that LeMir plays an important role in regulating a variety of HR types (Xu et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Analysis of protein profile of tomato root infected with Fusarium oxysporum f. sp lycopersici

Silva¹,

Almeida²,

Malafaia³

et al. 2017

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Fusarium wilt caused by Fusarium oxysporum f. sp lycopersici (Fol) is one of the main diseases affecting tomatoes. The BHRS 2,3 genotype of tomato is, however, resistant to this disease. A proteomic approach was used to understand the defense mechanisms of this genotype using the tomato root, the first tissue that interacts with the fungus, as a target. Protein was extracted and separated by two-dimensional electrophoresis followed by staining with Coomassie brilliant blue. The proteins were identified by MALDI-TOF/TOF mass spectrometry. A total of 22 proteins were identified, 21 of which showed differential expression with 12 proteins being upregulated and nine being downregulated. Plants responded to the pathogen with increased expression of pathogenesis-related proteins. We noted the induction of proteins involved in hypersensitivity reaction and other defense mechanisms. The expression of proteins of primary metabolism related to energy production, however, decreased, as did the expression of two proteins related to defense against abiotic stress. These results demonstrate the presence of important mechanisms for defense against Fol in the tomato genotype BHRS 2,3.

show abstract

“…LeMir, a putative miraculin gene in tomato, was induced by a rootknot nematode. The encoded protein product might help inhibit penetration of microorganisms into tomato roots (Brenner et al 1998). Transgenic tobacco plants expressing a cowpea trypsin inhibitor gene showed enhanced levels of insect resistance to a variety of insect pests (Boulter et al 1989).…”

Section: Tumor-related Proteinmentioning

confidence: 99%

Down-regulated gene expression of cold acclimated Poncirus trifoliata

Zhang¹,

Lang²,

Ebel³

et al. 2005

Can. J. Plant Sci.

View full text Add to dashboard Cite

. 2005. Down-regulated gene expression of cold acclimated Poncirus trifoliata. Can. J. Plant Sci. 85: 417-424. Citrus sp. are important commercial fruit crops throughout the world that are occasionally devastated by subfreezing temperatures. Poncirus trifoliata (maximum freeze tolerance of -26°C) is a close relative of commercial Citrus sp. (maximum freeze tolerance of -10°C) that has been used in breeding programs to develop more cold-hardy genotypes and as a rootstock to enhance freeze tolerance of the scion. Species with greater freeze tolerance vary in gene expression during cold acclimating temperatures. mRNA differential display (DDRT-PCR) and quantitative relative RT-PCR were used to study down regulation of gene expression in intact P. trifoliata exposed to a gradual cold acclimation regime to enhance our understanding of the mechanism that makes this specie so freeze tolerant. Six down-regulated genes were isolated and sequenced. These down-regulated genes showed high homology to the following known genes: chlorophyll a/b binding protein, photosystem II OEC 23, carbonic anhydrase, tumor related protein, pyrrolidone-carboxylate peptidase and β-galactosidase. Photoprotection and the global control of gene expression related to photosynthesis appear to be important mechanisms for cold acclimation of P. trifoliata. Poncirus trifoliata (tolérance maximale au gel de -26°C) est un proche parent du genre Citrus (tolérance maximale au gel de -10°C) employé dans les programmes d'hybridation pour produire des génotypes plus rustiques et des porte-greffe qui accroîtront la tolérance au gel du greffon. L'expression des gènes des espèces qui tolèrent bien le froid varie lors des températures conduisant à l'acclimatation. Les auteurs ont recouru au criblage différentiel de l'ARNm (DDRT-PCR) et à la PCR-CDNA quantitative relative pour étudier la régulation en aval de l'expression des gènes chez les plants de P. trifoliata restés intacts après une acclimatation progressive au froid afin de nous aider à mieux comprendre le mécanisme par lequel cette espèce réussit à survivre si bien au gel. Ils ont ainsi identifié et séquencé six gènes régulés en aval. Ces gènes se caractérisent par une grande homologie avec les gènes connus suivants : protéine a/b de liaison à la chlorophylle, photosystème II OEC 23, anhydrase carbonique, protéine associée aux tumeurs, pyrrolidone-carboxylate peptidase et β-galactosidase. La photoprotection et le contrôle général de l'expression des gènes associés à la photosynthèse semblent jouer un rôle important dans l'adaptation de P. trifoliata au froid.

show abstract

Characterization ofLeMir, a Root-Knot Nematode-Induced Gene in Tomato with an Encoded Product Secreted from the Root1

Cited by 64 publications

References 66 publications

Gene expression profiling of systemically wound-induced defenses in hybrid poplar

Gene expression profiling of systemically wound-induced defenses in hybrid poplar

Analysis of protein profile of tomato root infected with Fusarium oxysporum f. sp lycopersici

Down-regulated gene expression of cold acclimated Poncirus trifoliata

Contact Info

Product

Resources

About