Characterization of in vitro cultured bone marrow and adipose tissue‐derived mesenchymal stem cells and their ability to express neurotrophic factors

Abstract

MSCs (mesenchymal stem cells) have attracted attention as a promising tool for regenerative medicine and transplantation therapy. MSCs exert neuroprotective effects by secreting a number of factors in vitro and in vivo. Similar characteristics are found in ADSCs (adipose-derived stem cells) and BMSCs (bone marrow stromal cells). Multipotent capability, easy accessibility and rapid proliferation of ADSCs have been established. Our main objective was to compare cell viability, growth rate, expression of neurotrophic factors and nestin genes in ADSCs and BMSCs. Cell doubling time and proliferation rate indicate that ADSCs has a higher proliferation rate than BMSCs. ADSCs and BMSCs express a similar pattern of CD71 and CD90 markers. Nestin immunostaining showed that ADSCs and BMSCs are immunopositive. The expression of neurotrophic factors genes in ADSCs proved similar to that of BMSCs genes. Thus adipose tissue stem cells with a high proliferation rate can express nestin and neurotrophic factor genes. Therefore ADSCs may be useful in future cell replacement therapies and help improve neurodegenerative diseases.

Mesenchymal Stem/Stromal Cells in Liver Fibrosis: Recent Findings, Old/New Caveats and Future Perspectives

“…Interestingly, differences in proliferation rate were seen among MSCs obtained from diverse tissues [88]. In order to reach sufficient amount of cells at early passages, new strategies have recently been applied.…”

The Effect of Osteopontin on Microglia

“…Yet, its effect on microglia proliferation is still controversial. Tambuyzer et al found that the proliferation of microglial significantly increased compared with control cells when a lower concentration (10 fM) recombinant OPN was added to the culture medium, which indicated that OPN also stimulated proliferation of microglia [18]. However, Rabenstein et al put forward the opposite conclusion.…”

“…Treating these cells with OPN for 24 hours significantly increased the phagocytotic activity compared with microglia cultured in control medium. The uptake of beads by microglia treated with 1 nM OPN was almost doubled that of control cells, thereby largely restoring the activity to the level observed immediately after harvesting [18]. Therefore, they believe that OPN can increase the phagocytic activity of microglia.…”

“…In a variety of pathological conditions of CNS, such as brain trauma [11], cerebral ischemia [12], infection [13], and degenerative diseases [14], microglia can rapidly participate in the pathophysiology of brain damage via its activation, proliferation, migration, phagocytosis, and expression of inducible nitric oxide synthase (iNOS), nitric oxide (NO), and a number of proinflammatory cytokines [15]. Based on the effect of OPN and microglia reported recently, most studies suggest that OPN play a role in pathogenesis of neurodegenerative diseases or in neuroprotection by regulating the activation and function of microglia [16–18]. Thus, we will sum up the effect of OPN on microglia in several aspects including proliferation, migration, phagocytosis, and expression of proinflammatory cytokines.…”

Trophic factors from adipose tissue-derived multi-lineage progenitor cells promote cytodifferentiation of periodontal ligament cells

“…In contrast, mesenchymal stem cells (MSCs) from adipose tissue can be harvested less invasively and with greater ease compared to other tissues listed above. In addition, these cells have a higher selfrenewal capacity and proliferative ability [8e10], and produce an abundance of humoral factors [11,12]. By transplanting adipose tissue-derived multi-lineage progenitor cells (ADMPCs) into canine experimental periodontal disease models, we have demonstrated that ADMPC induces significant periodontal tissue regeneration [13].…”