Characterization of <i>Cylindrocarpon</i> Species Associated with Black Foot Disease of Grapevine in Spain

Alaniz, Sandra; León, Maela; Civera, Antonio Vicent; García‐Jiménez, J.; Abad-Campos, Paloma; Armengol, Josep

doi:10.1094/pdis-91-9-1187

Cited by 59 publications

(59 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Observation of the conidia revealed morphological characteristics that matched previous descriptions of Neonectria species [3,4,12]. The macroconidia had a mean measurement of 28.9 × 6.2 µm length and width respectively and were cylindrical, straight with one to three-septate (Figure 4) but the isolate did not produce microconidia.…”

Section: Characteristics Of Isolates and The Conidia Sizesupporting

confidence: 73%

“…Investigation should include variations among species of Neonectria, that are present in the state; host specificity, potential phenotypic and/or genotypic differentiation in comparison to previous reports from other locations [3]. It is also essential that further studies be conducted to provide understanding on the type of interactions existing between this pathogen and other pathogens involved in dry root rot of citrus.…”

Section: Conclusion and Recommendationmentioning

confidence: 98%

“…Isolates obtained were identified through anamorph morphology characteristics [3,4,11,12]. Digital images of 20 conidia of isolates were captured with a Leica DFC420 camera mounted on a compound microscope (Olympus BX40).…”

Section: Morphology and Molecular Identification Of Isolatesmentioning

confidence: 99%

“…The length and width of the conidia were determined using the SPOT Imaging Software (v4.7.0.35, Diagnostic Instruments, Inc., MI). The conidia characters were compared with those in previous reports [3,4,12] and isolates were subjected to molecular identification.…”

Section: Morphology and Molecular Identification Of Isolatesmentioning

confidence: 99%

“…Cylindrocarpon species usually infect their hosts through wounds on the roots and other below ground portions of the rootstock and stress may induce the disease. Neonectria macrodidyma and three other members of the genus (C. destructans, C. liriodendra, or C. macrodidyma) have been reported in association with black foot disease of grapevine in nurseries and vineyards in many countries, including Australia, Canada, Chile, New Zealand, South Africa, Spain, Tasmania, United States, and Uruguay [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Association of Neonectria macrodidyma with Dry Root Rot of Citrus in California

AO¹,

JS²,

BB³

et al. 2017

J Plant Pathol Microbiol

View full text Add to dashboard Cite

During yearly surveys that started in 2010 to identify pathogens associated with dry root rot disease of citrus in California, samples with root rot symptoms were collected in Tulare County. Small pieces of tissue from root samples were plated onto potato dextrose agar amended with 0.01% tetracycline and incubated at 25°C. Pure cultures of fungal isolates were identified by morphology and sequence analysis of the Internal Transcribed Spacer and Beta Tubulin regions. Neonectria macrodidyma (Cylindrocarpon macrodidymum) was first recovered in 2011 and has subsequently been recovered multiple times from citrus samples. The pathogen appeared to be widely distributed in association with citrus dry root rot and possibly interacts with Fusarium solani, Phytophthora nicotianae and P. citrophthora, the major soil borne pathogens that were frequently identified from plant samples with root and/or crown rot of citrus in California. The fungal genus Cylindrocarpon (Teleomorph: Neonectria wolenw=Dactylonect ria=Ilyonectria) contains ubiquitous soil borne pathogens that cause black foot disease on a wide range of hosts, including grapevine, strawberry, apple, and conifers. Hosts typically become infected through natural wounds on roots and other below ground parts. In this report, we present strain UCR3312, which is the most recently isolated pathogenic strain in 2015. Considering the potential damage that this organism may cause to the citrus industry, detailed studies are recommended to better understand its distribution, epidemiology, and the general pathogen biology to improve the disease management practices.

show abstract

Section: Characteristics Of Isolates and The Conidia Sizesupporting

confidence: 73%

Section: Conclusion and Recommendationmentioning

confidence: 98%

Section: Morphology and Molecular Identification Of Isolatesmentioning

confidence: 99%

Section: Morphology and Molecular Identification Of Isolatesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Association of Neonectria macrodidyma with Dry Root Rot of Citrus in California

AO¹,

JS²,

BB³

et al. 2017

J Plant Pathol Microbiol

View full text Add to dashboard Cite

show abstract

Investigation of Trichoderma species colonization of nursery grapevines for improved management of black foot disease

Jaarsveld

Halleen

Bester

et al. 2020

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND: Black foot disease (BFD) is one of the main fungal diseases associated with young grapevine decline. Trichoderma holds the potential to be used as biocontrol agent against this disease, though variable success of colonization were found when applied to nursery vines in previous studies. Therefore, field experiments were established to evaluate different methods of application of Trichoderma atroviride, and to evaluate the efficacy of different commercial Trichoderma products on BFD in nursery vines post callusing over two seasons. RESULTS: Only in one season of the trial evaluating different products did all of the Trichoderma treatments significantly lower the black foot infections in the rootstock bases of the vines (mean black foot pathogen incidence of 1.00 to 2.50% in Trichoderma treated vines versus 6.50% in the untreated control). When comparing tissue parts, the base of the vine and collar roots had significantly higher Trichoderma colonization than the middle and root tip parts. Significantly less BFD pathogens were isolated from the base in comparison to the roots. These colonization trends were found for both field trials over both seasons. The different application methods showed that dipping of basal ends in the dry formulation followed by monthly soil drenches consistently gave higher colonization [mean Trichoderma incidence in the bases were 39.20% (2016/2017) and 28.00% (2017/2018)], while the 1 h soak of the bases of vines was not effective [mean Trichoderma incidence in the bases were 8.80% (2016/2017) and 4.00% (2017/2018)] and did not differ from the untreated control. CONCLUSION: Even though Trichoderma spp. were not sufficient to prevent infections by BFD pathogens, a certain degree of protection was obtained in the basal ends, which may contribute to longevity of the vines once planted in the vineyard.

show abstract

Fungi in Roots of Nursery Grown Pinus sylvestris: Ectomycorrhizal Colonisation, Genetic Diversity and Spatial Distribution

Menkis

Vasaitis

2010

Microb Ecol

View full text Add to dashboard Cite

The aims of this study were to investigate patterns of ectomycorrhizal (ECM) colonisation and community structure on nursery grown seedlings of Pinus sylvestris, spatial distribution of ECMs in the nursery plot and genetic diversity of commonly isolated ECM basidiomycete Hebeloma cavipes. One hundred seedlings were sampled in 225 m(2) area using a systematic grid design. For each seedling, 20 individual root tips were randomly collected, morphotyped, and surface sterilised for fungal isolation in pure culture. Results showed that ECM community was comprised of nine distinct morphotypes among which Thelephora terrestris (39.7%), Hebeloma sp. (17.8%) and Suillus luteus (6.1%) were the most abundant. Spatial distribution of ECMs in the nursery plot was determined by their relative abundance: even in common ECMs and random in rare ones. Fungal isolation yielded 606 pure cultures, representing 71 distinct taxa. The most commonly isolated fungi were the ascomycetes Neonectria macrodidyma (20.3%), Phialocephala fortinii (13.5%), Neonectria radicicola (6.3%) and the ECM basidiomycete H. cavipes (4.5%). Intraspecific genetic diversity within 27 H. cavipes isolates was studied using two methods: restriction digestion of the amplified intergenic spacer of nuclear ribosomal DNA and genealogical concordance of five genetic markers. Five and eight genotypes were revealed by each respective method, but both of those were largely consistent, in particular, in determining the largest genotype (A) composed of 18 isolates. Mapping positions for each H. cavipes isolate and genotype in the field showed that isolates of the A genotype covered a large part of the nursery plot. This suggests that H. cavipes is largely disseminated by vegetative means of local genotypes and that nursery cultivation practices are likely to contribute to the dissemination of this species in the forest nursery soils.

show abstract

Characterization of Cylindrocarpon Species Associated with Black Foot Disease of Grapevine in Spain

Cited by 59 publications

References 15 publications

Association of Neonectria macrodidyma with Dry Root Rot of Citrus in California

Association of Neonectria macrodidyma with Dry Root Rot of Citrus in California

Investigation of Trichoderma species colonization of nursery grapevines for improved management of black foot disease

Fungi in Roots of Nursery Grown Pinus sylvestris: Ectomycorrhizal Colonisation, Genetic Diversity and Spatial Distribution

Contact Info

Product

Resources

About