Characterization of<i>Campylobacter jejuni</i>and<i>Campylobacter coli</i>Broiler Isolates by Whole-Genome Sequencing

Cantero, Guillermo; Correa‐Fiz, Florencia; Ronco, Troels; Strube, Mikael Lenz; Cerdà‐Cuéllar, Marta; Pedersen, Karl

doi:10.1089/fpd.2017.2325

Cited by 33 publications

(45 citation statements)

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“…Several multifactorial systems in Campylobacter contribute to pathogenicity and virulence in humans, and to survival in broilers and throughout the food chain (Bolton, 2015 ). Our finding that multiple virulence factors are highly prevalent in C. jejuni is with agreement with previous studies (Koolman et al, 2015 ; Cantero et al, 2017 ). The exception is T6SS that is lineage-related.…”

Section: Discussionsupporting

confidence: 94%

Genomic Epidemiology of Campylobacter jejuni Transmission in Israel

et al. 2018

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Objectives: Campylobacter jejuni is responsible for 80% of Campylobacter infections in Israel, a country with a high incidence reaching 91/100,000 population. We studied the phylogeny, diversity and prevalence of virulence factors using whole genome sequencing (WGS) of a national sample of C. jejuni clinical, food, and animal isolates collected over a 10-year period (2003–2012).Methods: C. jejuni isolates (n = 263) were subject to WGS using Illumina sequencing (PE 250bpx2). Raw reads and de novo assemblies were analyzed with the BioNumerics whole genome MLST (wgMLST) pipeline. Reads were screened for 71 virulence genes by the SRST2 script. Allelic profiles were analyzed to create minimum spanning trees and allelic core distances were investigated to determine a reliable cutoff for strain determination.Results: wgMLST analysis of 263 C. jejuni isolates indicated significant diversity among the prevalent clonal complexes (CCs) with CC-21 and CC-353 being the most diverse, and CC-574 the most clonal. Within CC-21, sequence type (ST)-1359 created a separate clade. Human, poultry and bovine isolates clustered together across the different STs. Forty four percent of studied isolates were assigned to 29 genetic clusters. Temporal and geographical relatedness were found among the minority of clusters, while most phylogenetically associated cases appeared diffuse and unassociated epidemiologically. The majority of virulence factors were highly prevalent across the dataset and not associated with genotype, source of isolation or invasiveness. Conversely, all 13 genes associated with type VI secretion system (T6SS) were lineage-related and identified in only 18% of the isolates. T6SS was detected in 95.2% of ST-1359, a common type in Israel.Conclusions: wgMLST supported the assessment that poultry and cattle are likely food sources of infection in Israel. Substantial genetic clustering among C. jejuni isolates suggested multiple point source and diffuse outbreaks that were previously unreported in Israel. The high prevalence of T6SS among ST-1359 isolates is unique to Israel, and requires further investigation. This study exemplifies the importance of studying foodborne pathogens using advanced genomic approaches across the entire spectrum of One Health.

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Section: Discussionsupporting

confidence: 94%

Genomic Epidemiology of Campylobacter jejuni Transmission in Israel

et al. 2018

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“…In this study, the efflux pump system was observed in most C. jejuni e C. coli strains isolated from broilers. These results are in agreement with those of others authors from Brazil and other countries (Hungaro et al 2015, Lin et al 2002, Van Deun et al 2007, Cantero et al 2018 which frequently observed the presence of this efflux system in Campylobacter strains. Of the nine strains in which the full efflux system was not detected, one was isolated from a commercial abattoir and eight were from the poultry center located São José do Vale do Rio Preto.…”

Section: Resultssupporting

confidence: 94%

“…In fact, Panzenhagen et al (2016b) registered enrofloxacin wastes in 72.2% of broilers' samples collected in the state of Rio de Janeiro, analyzed by Enzyme-Linked Immunosorbent Assay (ELISA), albeit at rates lower than the Maximum Waste Limits. In Spain, Cantero et al (2018) reported that (Lin et al 2002) isolated strains of broilers were predominantly resistant to quinolones, perhaps due to the frequent use of enrofloxacin in poultry breeding. The 2016 EU´s report similarly described resistance to antimicrobial agents in zoonotic bacteria and in indicating bacteria from humans, animals and food.…”

Section: Resultsmentioning

confidence: 99%

Detection of efflux pump CmeABC in enrofloxacin resistant Campylobacter spp. strains isolated from broiler chickens (Gallus gallus domesticus) in the state of Rio de Janeiro, Brazil

et al. 2019

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Fowls are the main reservoirs of the highly important food-originating pathogen called Campylobacter spp. and broilers’ meat and byproducts are the main vehicles of this microorganism. Increasing of Campylobacter spp. resistant strains to fluorquinolones, an antimicrobial class often employed in poultry farming and in human medicine has become a great concern to poultry breeders. In fact, several studies evaluated increasing bacterial resistance against these antimicrobial agents. The role of CmeABC efflux system has been underscored among the resistance mechanisms in Campylobacter spp. to fluorquinolones. This study investigated the occurrence of CmeABC efflux pump in 81 and 78 enrofloxacin resistant strains of Campylobacter jejuni and C. coli respectively, isolated from broilers collected from six abattoirs situated at São José do Vale do Rio Preto/RJ poultry center and from two commercial abattoirs situated at Metropolitan Region of Rio de Janeiro, from 2013 to 2016. The resistance to enrofloxacin was assessed by agar dilution to determine minimum inhibitory concentration (MIC). The CmeABC efflux system was investigated through the detection of genes genes cmeA, cmeB and cmeC by PCR. The activity of CmeABC efflux pump was investigated in 20 strains by using the efflux pump inhibitor Phenylalanine-Arginine β-Naphthylamide (PAβN). The three genes cmeA, cmeB and cmeC were detected in 94.3% of the strains (C. jejuni = 80 and C. coli = 70), whereas the system was absent or incomplete in 5.7% of strains (C. jejuni = 1 and C. coli = 8). MIC varied between 0.5μg/ml and 64μg/ml, and 88.7% of strains were enrofloxacin resistant and 11.3% featuring intermediate resistance. The inhibition of the efflux pump by PAβN reduced the MIC to enrofloxacin up to eight times in fifteen strains (75%). These results indicate that this system is frequent and active in Campylobacter spp. Resistant strains in the presence of enrofloxacin.

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“…In recent Catalonian (north-east Spain) and Italian studies, the predominant clonal complex of C . coli isolates was also ST828 [ 20 , 21 ]…”

Section: Resultsmentioning

confidence: 99%

Phylogenetic analyses and antimicrobial resistance profiles of Campylobacter spp. from diarrhoeal patients and chickens in Botswana

et al. 2018

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Campylobacter spp. are a leading cause of bacterial enteritis worldwide, including countries in Africa, and have been identified by the World Health Organisation (WHO) as one of the high priority antimicrobial resistant pathogens. However, at present there is little knowledge on the prevalence, molecular epidemiology or antimicrobial susceptibility of Campylobacter spp. isolates in Botswana, both in patients and in the zoonotic context. Some data indicate that ~14% of diarrhoeal disease cases in a paediatric setting can be ascribed to Campylobacter spp., urging the need for the magnitude of Campylobacter-associated diarrhoea to be established. In this survey, we have characterised the genomic diversity of Campylobacter spp. circulating in Botswana isolated from cases of diarrhoeal disease in humans (n = 20) and from those that colonised commercial broiler (n = 35) and free-range (n = 35) chickens. Phylogeny showed that the Campylobacter spp. isolated from the different poultry and human sources were highly related, suggesting that zoonotic transmission has likely occurred. We found that for Campylobacter spp. isolated from humans, broilers and free-range chickens, 52% was positive for tetO, 47% for gyrA-T86I, 72% for blaOXA-61, with 27% carrying all three resistance determinants. No 23S mutations conferring macrolide resistance were detected in this survey. In summary, our study provides insight into Campylobacter spp. in poultry reservoirs and in diarrhoeal patients, and the relevance for treatment regimens in Botswana.

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Characterization ofCampylobacter jejuniandCampylobacter coliBroiler Isolates by Whole-Genome Sequencing

Cited by 33 publications

References 42 publications

Genomic Epidemiology of Campylobacter jejuni Transmission in Israel

Genomic Epidemiology of Campylobacter jejuni Transmission in Israel

Detection of efflux pump CmeABC in enrofloxacin resistant Campylobacter spp. strains isolated from broiler chickens (Gallus gallus domesticus) in the state of Rio de Janeiro, Brazil

Phylogenetic analyses and antimicrobial resistance profiles of Campylobacter spp. from diarrhoeal patients and chickens in Botswana

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