Characterization of Burkholderia sp. XTB‐5 for Phenol Degradation and Plant Growth Promotion and Its Application in Bioremediation of Contaminated Soil

Abstract

A key issue when researching land degradation is the pollution of soils. For bioremediation of contaminated soil, Burkholderia sp. XTB‐5 cells were obtained from soil and grown on mineral salt medium with initial phenol concentrations of 650 mg L−1 and 850 mg L−1, which were found to degrade more than 98% of phenol content in less than 4 days. About 90% of phenol content (with initial concentration of 250 mg kg−1 soil) was removed from soil inoculated with XTB‐5 cells in 6 days. More than 90% of phenol content was removed within 20 days after co‐introduction of XTB‐5 cells and plants to sterilized soil in a greenhouse or to natural soil in field trials. But under the same conditions, individual introduction of plants to sterilized soil in the greenhouse reduced phenol content by about 50% and introduction to natural soil in field trials reduced phenol content by about 38%, suggesting that phytoremediation of phenol is often inefficient and microorganisms can efficiently degrade this pollutant. In addition, strain XTB‐5 was found to solubilize phosphate and produce 1‐Aminocyclopropane‐1‐Carboxylate (ACC) deaminase and siderophore. Strain XTB‐5 promoted plant growth in both phenol‐absent and phenol‐spiked soil under greenhouse and field conditions. Considering that ACC deaminase is beneficial to plant growth under adverse environmental conditions, plant growth promotion by XTB‐5 in phenol‐contaminated soil is not only due to XTB‐5 cell‐degradation of phenol and reduced phytotoxicity but also to production of ACC deaminase. Hence, Burkholderia sp. XTB‐5 presents an attractive microorganism for phytoremediation of contaminated soil and agronomic application. Copyright © 2016 John Wiley & Sons, Ltd.

Cell-Free Fermentation Broth of Bacillus velezensis Strain S3-1 Improves Pak Choi Nutritional Quality and Changes the Bacterial Community Structure of the Rhizosphere Soil

“…The bulk soil was collected by shaking the roots of the plants, while the rhizosphere soil was collected by brushing (Chen et al, 2016). Six Pak choi were taken from each plot to collect rhizosphere soil for mixing.…”

Mobilization of recalcitrant phosphorous and enhancement of pepper P uptake and yield by a new biocontrol and bioremediation bacterium Burkholderia cepacia CQ18

“…cepacia strains can use these toxic compounds as a carbon source and at the same time SIP is released via dephosphorylation by phosphatases, removing those chemicals from environments (Patil and Jena 2016; Chen et al . 2017; Shahid and Khan 2018).…”

“…In addition, phosphonated phenols are commonly used in agriculture as pesticides, herbicides and preservatives, which are a major group of recalcitrant environmental pollutants. Some B. cepacia strains can use these toxic compounds as a carbon source and at the same time SIP is released via dephosphorylation by phosphatases, removing those chemicals from environments (Patil and Jena 2016;Chen et al 2017;Shahid and Khan 2018).…”

Microstructural changes in soils induced by wetting and drying: Effects on atrazine mobility

“…Such measurements may unveil the direct effect of WDC on aggregate degradation/stability. Subsequent in‐depth understanding will enable to identify indirect effects resulting from water diffusion in/out of aggregates, including pesticide migration, a key process associated with land degradation (Chen et al, ; Rodríguez‐Seijo, Alfaya, Andrade, & Vega, ).…”