Characterization of <i>Burkholderia</i> sp. strain CJ1, a newly isolated berberine-degrading bacterium from rhizosphere of <i>Coptis japonica</i>

Yoshida, Hinaka; Takeda, Hisashi; Wakana, Daigo; Hosoe, Tomoo

doi:10.1080/09168451.2020.1721264

Cited by 2 publications

(1 citation statement)

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“…When BBR enters water through runoff, leaching and wastewater discharge [ 8 , 10 ], the degradation of BBR in water and its impact on bacterial diversity in the water environment need further investigation. It has been confirmed that the microorganisms that can degrade BBR include Sphingopyxis [ 11 ], Burkholderia [ 12 ], Rhodococcus [ 13 ] and Bacillus [ 6 ]. Mixed cultures of Hydrogenophaga , Azoarcus , Sphingopyxis , Stenotrophomonas , Shinella , Alcaligenes and Nitrospir are identified as potential functional species for the biodegradation of BBR and its metabolites [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Tetrahydroisoquinoline N-methyltransferase from Methylotenera Is an Essential Enzyme for the Biodegradation of Berberine in Soil Water

Chen

Liu

et al. 2022

Molecules

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Berberine (BBR), a Chinese herbal medicine used in intestinal infection, has been applied as a botanical pesticide in the prevention of fungal disease in recent years. However, its degradation in the environment remains poorly understood. Here, we investigated BBR’s degradation in soil water from different sources accompanied by its effect on bacterial diversity. Our results indicated that BBR was only degraded in soil water, while it was stable in tap water, river water and aquaculture water. Bacterial amplicon results of these samples suggested that the degradation of BBR was closely related to the enrichment of Methylotenera. To reveal this special relationship, we used bioinformatics tools to make alignments between the whole genome of Methylotenera and the pathway of BBR’s degradation. An ortholog of Tetrahydroisoquinoline N-methyltransferase from plant was discovered only in Methylotenera that catalyzed a crucial step in BBR’s degradation pathway. In summary, our work indicated that Methylotenera was an essential bacterial genus in the degradation of BBR in the environment because of its Tetrahydroisoquinoline N-methyltransferase. This study provided new insights into BBR’s degradation in the environment, laying foundations for its application as a botanical pesticide.

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